{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:58:22Z","timestamp":1777510702297,"version":"3.51.4"},"reference-count":46,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Cellular Reprogramming"],"published-print":{"date-parts":[[2021,10,1]]},"abstract":"\n We examined the effects of treatment with pulsed electromagnetic fields (PEMFs) on cumulus cells and buffalo somatic cell nuclear transfer (SCNT) embryos. PEMF treatment (30\u2009\u03bcT for 3 hours) of cumulus cells increased (\n p<\/jats:italic>\n \u2009<\u20090.05) the relative cell viability and cell proliferation and the expression level of\n OCT4<\/jats:italic>\n ,\n NANOG<\/jats:italic>\n ,\n SOX2<\/jats:italic>\n ,\n P53<\/jats:italic>\n ,\n CCNB1<\/jats:italic>\n , and\n GPX<\/jats:italic>\n , but decreased (\n p<\/jats:italic>\n \u2009<\u20090.05) that of\n DNMT1<\/jats:italic>\n ,\n DNMT3a<\/jats:italic>\n ,\n GSK3b<\/jats:italic>\n , and\n BAX<\/jats:italic>\n , whereas the expression level of\n DNMT3b<\/jats:italic>\n ,\n GLUT1<\/jats:italic>\n ,\n BCL2<\/jats:italic>\n ,\n CASPASE3<\/jats:italic>\n ,\n SOD1<\/jats:italic>\n , and\n CATALASE<\/jats:italic>\n was not affected. PEMF treatment of SCNT embryos at the beginning of\n in vitro<\/jats:italic>\n culture increased (\n p<\/jats:italic>\n \u2009<\u20090.05) the blastocyst rate (51.4%\u2009\u00b1\u20091.36% vs. 42.8%\u2009\u00b1\u20091.29%) and decreased (\n p<\/jats:italic>\n \u2009<\u20090.01) the apoptotic index to the level in\n in vitro<\/jats:italic>\n fertilization blastocysts, but did not significantly alter the total cell number and the inner cell mass:trophectoderm cell number ratio of blastocysts compared to the controls. PEMF treatment increased the expression level of\n NANOG<\/jats:italic>\n ,\n SOX2<\/jats:italic>\n ,\n CDX2<\/jats:italic>\n ,\n GLUT1<\/jats:italic>\n ,\n P53<\/jats:italic>\n , and\n BCL2<\/jats:italic>\n and decreased that of\n BAX<\/jats:italic>\n ,\n CASPASE3<\/jats:italic>\n ,\n GSK3b<\/jats:italic>\n , and\n HSP70<\/jats:italic>\n , but not\n OCT4<\/jats:italic>\n ,\n DNMT1<\/jats:italic>\n ,\n DNMT3a<\/jats:italic>\n ,\n DNMT3b<\/jats:italic>\n ,\n HDAC1<\/jats:italic>\n , and\n CCNB1<\/jats:italic>\n in blastocysts. It increased (\n p<\/jats:italic>\n \u2009<\u20090.001) the global level of H3K27me3 but not H3K18ac. These results suggest that PEMF treatment of SCNT embryos improves their developmental competence, reduces the level of apoptosis, and alters the expression level of several important genes related to pluripotency, apoptosis, metabolism, and stress.\n <\/jats:p>","DOI":"10.1089\/cell.2021.0028","type":"journal-article","created":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T16:07:43Z","timestamp":1633104463000},"page":"304-315","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Exposure to Pulsed Electromagnetic Fields Improves the Developmental Competence and Quality of Somatic Cell Nuclear Transfer Buffalo (\n Bubalus bubalis<\/i>\n ) Embryos Produced Using Fibroblast Cells and Alters Their Epigenetic Status and Gene Expression"],"prefix":"10.1177","volume":"23","author":[{"given":"Aditya Kumar","family":"Sharma","sequence":"first","affiliation":[{"name":"Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shrutika","family":"Sah","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suresh Kumar","family":"Singla","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manmohan Singh","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radhey Shyam","family":"Manik","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2469-7466","authenticated-orcid":false,"given":"Prabhat","family":"Palta","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, India."},{"name":"Embryo Biotechnology Laboratory, Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal, India."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2021,10,12]]},"reference":[{"key":"e_1_3_5_2_1","first-page":"S55","article-title":"Evaluating the quality of individual embryos with a non-invasive and highly sensitive measurement of oxygen consumption by scanning electrochemical microscopy","volume":"52","author":"Abe H.","year":"2006","unstructured":"Abe H. 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