{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:08:55Z","timestamp":1777511335635,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2009,1,23]],"date-time":"2009-01-23T00:00:00Z","timestamp":1232668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cloning and Stem Cells"],"published-print":{"date-parts":[[2009,3,1]]},"abstract":"\n Somatic cell nuclear transfer enables the generation of embryonic stem cells (ESCs) that genetically match the donor and can be used to treat disease through cell replacement therapies or to recapitulate patient-specific disease via\n in vitro<\/jats:italic>\n differentiation. We performed a \u201cproof-of-principle\u201d study using tail tip fibroblasts from a mouse model of Parkinson's disease (\n Aphakia<\/jats:italic>\n ) as the donor cell nuclei for nuclear transfer and derived \u201ccustomized\u201d ESCs for\n in vitro<\/jats:italic>\n analysis.\n Aphakia<\/jats:italic>\n mice contain deletions in the\n pitx3<\/jats:italic>\n gene and show selective loss of dopamine neurons of the substantia nigra, specifically the neuron population susceptible to degeneration in Parkinson's disease. Using electrofusion nuclear transfer, we produced cloned\n Aphakia<\/jats:italic>\n oocytes at rates similar to those for control, cloned oocytes.\n Aphakia<\/jats:italic>\n ESCs were isolated and live mice were generated using tetraploid embryo complementation.\n In vitro<\/jats:italic>\n differentiation of\n Aphakia<\/jats:italic>\n ESCs to dopaminergic neurons revealed significantly fewer TH\n +<\/jats:sup>\n neurons that expressed MAP2, DAT, synaptophysin, VMAT2, and AHD2 compared to control nuclear transfer ESC cultures, supporting a role for Pitx3 in mesodiencephalic dopamine neuron maturation. Taken together, our studies define a customized\n in vitro<\/jats:italic>\n ESC culture system used to analyze gene-specific contribution to dopamine neuron generation, maturation, and susceptibility to degeneration.\n <\/jats:p>","DOI":"10.1089\/clo.2008.0059","type":"journal-article","created":{"date-parts":[[2009,2,12]],"date-time":"2009-02-12T16:18:07Z","timestamp":1234455487000},"page":"77-88","source":"Crossref","is-referenced-by-count":9,"title":["Nuclear Transfer Embryonic Stem Cells Provide an\n In Vitro<\/i>\n Culture Model for Parkinson's Disease"],"prefix":"10.1177","volume":"11","author":[{"given":"Tomokazu","family":"Amano","sequence":"first","affiliation":[{"name":"Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodora","family":"Papanikolaou","sequence":"additional","affiliation":[{"name":"Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut."},{"name":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Ying","family":"Sung","sequence":"additional","affiliation":[{"name":"Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut."},{"name":"Institute of Biotechnology, National Taiwan University, Taipei 106, Taiwan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Lennington","sequence":"additional","affiliation":[{"name":"Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut."},{"name":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne","family":"Conover","sequence":"additional","affiliation":[{"name":"Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut."},{"name":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangzhong","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut."},{"name":"Department of Animal Science, University of Connecticut, Storrs, Connecticut."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2009,1,23]]},"container-title":["Cloning and Stem Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/clo.2008.0059","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/clo.2008.0059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:40:08Z","timestamp":1777390808000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/clo.2008.0059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,23]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,3,1]]}},"alternative-id":["10.1089\/clo.2008.0059"],"URL":"https:\/\/doi.org\/10.1089\/clo.2008.0059","relation":{},"ISSN":["1536-2302","1557-7457"],"issn-type":[{"value":"1536-2302","type":"print"},{"value":"1557-7457","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1,23]]}}}