{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:27:08Z","timestamp":1789864028504,"version":"4.0.1"},"reference-count":39,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["The CRISPR Journal"],"published-print":{"date-parts":[[2021,12,1]]},"abstract":"<jats:p>\n                    Avian and human influenza viruses bind to porcine sialic acid receptors to generate novel viruses that pose a potential pandemic threat to public health. Evidence suggests that the host factors regulating the influenza virus life cycle and viral reassortment are potential broad-spectrum antiviral drug targets, compared to the ineffective seasonal vaccines against highly pathogenic viruses, leading to drug resistance. After performing a genome-wide CRISPR-Cas9 screen targeting 13,735 genes in porcine cell lines, we identified several host factors critical for influenza virus infection\u2014notably, a conserved oligomeric Golgi complex protein, COG8, which regulates viral protein transport and immune factor expression. Viral titers indicated that the loss of\n                    <jats:italic toggle=\"yes\">COG8<\/jats:italic>\n                    significantly enhanced cellular resistance to influenza (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.005). Moreover,\n                    <jats:italic toggle=\"yes\">COG8<\/jats:italic>\n                    knockout reduced the colocalization between viral particles and early endosome marker (EEA1), indicating COG8's role in the early endosome trafficking events of the virus.\n                    <jats:italic toggle=\"yes\">COG8<\/jats:italic>\n                    deletion inhibited the retrograde transport from the endosome to the trans-Golgi network, thereby accumulating the influenza protein M2 in early endosomes.\n                    <jats:italic toggle=\"yes\">COG8<\/jats:italic>\n                    silencing enhanced the expression of immune-related genes, indicating COG8-mediated host immune responses affect virus replication. Our experiments have revealed COG8 as an essential factor in influenza virus infection.\n                  <\/jats:p>","DOI":"10.1089\/crispr.2021.0054","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T07:57:54Z","timestamp":1771919874000},"page":"872-883","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Porcine Genome-Wide CRISPR Screen Identifies the Golgi Apparatus Complex Protein COG8 as a Pivotal Regulator of Influenza Virus Infection"],"prefix":"10.1177","volume":"4","author":[{"given":"Ao","family":"Zhou","sequence":"first","affiliation":[{"name":"College of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, P.R. China"},{"name":"Basic Medical College, Southwest Medical University, Luzhou, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhua","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Tang","sequence":"additional","affiliation":[{"name":"Basic Medical College, Southwest Medical University, Luzhou, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,12,16]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08699"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.05.008"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07151"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08760"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1312374110"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.11.002"},{"key":"e_1_3_5_8_2","first-page":"158","article-title":"The pig as a mixing vessel for influenza viruses: human and veterinary implications","volume":"3","author":"Ma W","year":"2008","unstructured":"Ma W, , Kahn RE, , Richt JA. The pig as a mixing vessel for influenza viruses: human and veterinary implications. 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