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To study methoprene action in the yellow fever mosquito,\n                    <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                    , the\n                    <jats:italic toggle=\"yes\">E93<\/jats:italic>\n                    (ecdysone-induced transcription factor) was knocked out using the CRISPR-Cas9 system. The E93 mutant pupae retained larval tissues similar to methoprene-treated insects. These insects completed pupal ecdysis and died as pupa. In addition, the expression of transcription factors,\n                    <jats:italic toggle=\"yes\">broad complex<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Kr\u00fcppel homolog 1<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">Kr-h1<\/jats:italic>\n                    ), increased and that of programmed cell death (PCD) and autophagy genes decreased in\n                    <jats:italic toggle=\"yes\">E93<\/jats:italic>\n                    mutants. These data suggest that methoprene functions through JH receptor, methoprene-tolerant, and induces the expression of\n                    <jats:italic toggle=\"yes\">Kr-h1<\/jats:italic>\n                    , which suppresses the expression of\n                    <jats:italic toggle=\"yes\">E93<\/jats:italic>\n                    , resulting in a block in PCD and autophagy of larval tissues. Failure in the elimination of larval tissues and the formation of adult structures results in their death. These results answered long-standing questions on the mode of action of methoprene.\n                  <\/jats:p>","DOI":"10.1089\/crispr.2022.0066","type":"journal-article","created":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T13:52:00Z","timestamp":1668433920000},"page":"813-824","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["CRISPR-Cas9 Genome Editing Uncovers the Mode of Action of Methoprene in the Yellow Fever Mosquito,\n                    <i>Aedes aegypti<\/i>"],"prefix":"10.1177","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4544-4903","authenticated-orcid":false,"given":"Guan-Heng","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharath Chandra","family":"Gaddelapati","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaoyu","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinmo","family":"Koo","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0873-3247","authenticated-orcid":false,"given":"Subba Reddy","family":"Palli","sequence":"additional","affiliation":[{"name":"Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Lexington, Kentucky, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,12,12]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-011019-024918"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2017.11.026"},{"key":"e_1_3_6_4_2","unstructured":"WHO. 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