{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T08:53:56Z","timestamp":1788512036807,"version":"build-2803163510"},"reference-count":28,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T00:00:00Z","timestamp":1680307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["The CRISPR Journal"],"published-print":{"date-parts":[[2023,4,1]]},"abstract":"<jats:p>\n                    Advances in molecular medicine have placed nucleic acid detection methods at the center of an increasing number of clinical applications. Polymerase chain reaction (PCR)-based diagnostics have been widely adopted for their versatility, specificity, and sensitivity. However, recently reported clustered regularly interspaced short palindromic repeats-based methods have demonstrated equivalent to superior performance, with increased portability and reduced processing time and cost. In this study, we applied Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK) technology to the detection of oncogenic rearrangements. We implemented SHERLOCK for the detection of\n                    <jats:italic toggle=\"yes\">BCR::ABL1<\/jats:italic>\n                    mRNA, a hallmark of chronic myeloid leukemia (CML), and\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    DNA oncogenic alleles, frequently detected in glioblastoma and non-small cell lung cancer (NSCLC). SHERLOCK enabled rapid, sensitive, and variant-specific detection of\n                    <jats:italic toggle=\"yes\">BCR::ABL1<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">EGFR<\/jats:italic>\n                    alterations. Compared with the gold-standard PCR-based methods currently used in clinic, SHERLOCK achieved equivalent to greater sensitivity, suggesting it could be a new tool in CML and NSCLC, to detect low level of molecular residual disease.\n                  <\/jats:p>","DOI":"10.1089\/crispr.2022.0070","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T11:13:41Z","timestamp":1678706021000},"page":"140-151","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["Specific High-Sensitivity Enzymatic Reporter UnLOCKing-Mediated Detection of Oncogenic\n                    <i>BCR::ABL1<\/i>\n                    and\n                    <i>EGFR<\/i>\n                    Rearrangements"],"prefix":"10.1177","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3925-705X","authenticated-orcid":false,"given":"Gr\u00e9goire","family":"Cullot","sequence":"first","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014MoTRIL Team, INSERM U1312, University of Bordeaux, Bordeaux, France."},{"name":"Department of Biology, ETH Zurich, Zurich, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3238-5251","authenticated-orcid":false,"given":"Samuel","family":"Amintas","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014BioGo Team, INSERM U1312, University of Bordeaux, Bordeaux, France."},{"name":"Department of Tumor Biology and Tumor Library, CHU Bordeaux, Bordeaux, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Karemb\u00e9","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014BioGo Team, INSERM U1312, University of Bordeaux, Bordeaux, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Prouzet-Maul\u00e9on","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014MoTRIL Team, INSERM U1312, University of Bordeaux, Bordeaux, France."},{"name":"CRISP'edit, TBMCore, CNRS UAR3427, INSERM US005, University of Bordeaux, Bordeaux, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"R\u00e9billard","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014BioGo Team, INSERM U1312, University of Bordeaux, Bordeaux, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Boureau","sequence":"additional","affiliation":[{"name":"Laboratory of Hematology, CHU Bordeaux, Bordeaux, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Cappellen","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014BioGo Team, INSERM U1312, University of Bordeaux, Bordeaux, France."},{"name":"Department of Tumor Biology and Tumor Library, CHU Bordeaux, Bordeaux, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lie","family":"Bedel","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014BioGo Team, INSERM U1312, University of Bordeaux, Bordeaux, France."},{"name":"Department of Biochemistry and Molecular Biology, CHU Bordeaux, Bordeaux, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1813-3939","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Moreau-Gaudry","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014BioGo Team, INSERM U1312, University of Bordeaux, Bordeaux, France."},{"name":"Department of Biochemistry and Molecular Biology, CHU Bordeaux, Bordeaux, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Dulucq","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014MoTRIL Team, INSERM U1312, University of Bordeaux, Bordeaux, France."},{"name":"Laboratory of Hematology, CHU Bordeaux, Bordeaux, France."},{"name":"Fi-LMC Group, L\u00e9on B\u00e9rard Center, Lyon, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Dabernat","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014BioGo Team, INSERM U1312, University of Bordeaux, Bordeaux, France."},{"name":"Department of Biochemistry and Molecular Biology, CHU Bordeaux, Bordeaux, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Turcq","sequence":"additional","affiliation":[{"name":"Bordeaux Institute in Oncology\u2014BRIC\u2014MoTRIL Team, INSERM U1312, University of Bordeaux, Bordeaux, France."},{"name":"CRISP'edit, TBMCore, CNRS UAR3427, INSERM US005, University of Bordeaux, Bordeaux, France."},{"name":"Fi-LMC Group, L\u00e9on B\u00e9rard Center, Lyon, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,4,13]]},"reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2020.102019"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00241"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1609324"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-017-0045-7"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-011110-130324"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70233-3"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2016.68.2914"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdv270"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-020-0776-2"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.29"},{"issue":"1","key":"e_1_3_6_12_2","first-page":"593","article-title":"Evaluation 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Evaluation of EGFR mutations in NSCLC with highly sensitive droplet digital PCR assays. 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The proportion of different BCR-ABL1 transcript types in chronic myeloid leukemia. An international overview. Leukemia, 2019; 33(5):1173\u20131183; doi: 10.1038\/s41375-018-0341-4","journal-title":"An international overview. 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