{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:31:49Z","timestamp":1773307909927,"version":"3.50.1"},"reference-count":35,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["DNA and Cell Biology"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p>\n                    T-cell immunodeficiency is a common feature in patients with chronic myeloid leukemia (CML), and deficiency in CD3 levels was detected in T cells from these patients, which may represent a characteristic that is related to a lower T cell activation. In this study, we explored the possibility that forced\n                    <jats:italic toggle=\"yes\">TCR\u03b6<\/jats:italic>\n                    gene expression may upreg-u-late T cell receptor (TCR) signaling activation and reverse interleukin-2 (IL-2) production in T cells from patients with CML. A recombinant eukaryotic vector expressing\n                    <jats:italic toggle=\"yes\">TCR\u03b6<\/jats:italic>\n                    was transfected into T cells by nucleofection. Phosphorylated TCR\u03b6, phosphorylated NF-\u03baB, and the IL-2 level in\n                    <jats:italic toggle=\"yes\">TCR\u03b6<\/jats:italic>\n                    -transfected CD3+T cells that were activated with anti-CD3 and anti-CD28 antibodies were measured by Western blot and enzyme-linked immunosorbent assay (ELISA). Significantly increased TCR\u03b6 levels were found in\n                    <jats:italic toggle=\"yes\">TCR\u03b6<\/jats:italic>\n                    -transfected CD3+T cells. After CD3 and CD28 antibody stimulation, a significantly higher phosphorylated TCR\u03b6 chain level was demonstrated, and an increased IL-2 production in TCR\u03b6-upregulated T cells was associated with the increased expression of the phosphorylated NF-\u03baB. In conclusion,\n                    <jats:italic toggle=\"yes\">TCR\u03b6<\/jats:italic>\n                    gene transfection could restore TCR\u03b6 chain deficiency and enhance IL-2 production in T cells from patients with CML. It is possible that TCR\u03b6 chain reconstitution in leukemia-specific, clonally expanded T cells will effectively increase their activation of antileukemia cytotoxicity.\n                  <\/jats:p>","DOI":"10.1089\/dna.2012.1798","type":"journal-article","created":{"date-parts":[[2012,10,11]],"date-time":"2012-10-11T12:46:06Z","timestamp":1349959566000},"page":"1628-1635","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Upregulated TCR\u03b6 Enhances Interleukin-2 Production in T-Cells from Patients with CML"],"prefix":"10.1177","volume":"31","author":[{"given":"Xianfeng","family":"Zha","sequence":"first","affiliation":[{"name":"Jinan University, Guangzhou","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaohua","family":"Chen","sequence":"additional","affiliation":[{"name":"Jinan University, Guangzhou","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijian","family":"Yang","sequence":"additional","affiliation":[{"name":"Jinan University, Guangzhou","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Shi","sequence":"additional","affiliation":[{"name":"Jinan University, Guangzhou","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Li","sequence":"additional","affiliation":[{"name":"Jinan University, Guangzhou","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuli","family":"Wu","sequence":"additional","affiliation":[{"name":"Jinan University, Guangzhou","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhong","family":"Lu","sequence":"additional","affiliation":[{"name":"Jinan University, Guangzhou","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangqiu","family":"Li","sequence":"additional","affiliation":[{"name":"Jinan University, Guangzhou","place":["China"]},{"name":"Jinan University","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2012,11,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1038\/sj.emboj.7600245","article-title":"Stoichiometry of the T-cell receptor-CD3 complex and key intermediates assembled in the endoplasmic reticulum","volume":"23","author":"Call M.E.","year":"2004","unstructured":"Call M.E., , Pyrdol J., , Wucherpfennig K.W. 2004. Stoichiometry of the T-cell receptor-CD3 complex and key intermediates assembled in the endoplasmic reticulum. EMBO J, 23:2348\u20132357.","journal-title":"EMBO J"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115625"},{"key":"e_1_3_3_4_1","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.humimm.2012.02.018","article-title":"The role of BCL11B in regulating the proliferation of human naive T cells","volume":"73","author":"Chen S.","year":"2012","unstructured":"Chen S., , Huang X., , Chen S., , Yang L., , Shen Q., , Zheng H., , Li B., , Grabarczyk P., , Przybylski G.K., , Schmidt C.A., , Li Y. 2012. The role of BCL11B in regulating the proliferation of human naive T cells. Hum Immunol, 73:456\u2013464.","journal-title":"Hum Immunol"},{"key":"e_1_3_3_5_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1179\/102453309X385241","article-title":"TCR zeta chain expression in T cells from patients with CML","volume":"14","author":"Chen S.","year":"2009","unstructured":"Chen S., , Yang L., , Chen S., , Li Y. 2009. TCR zeta chain expression in T cells from patients with CML. Hematology, 14:95\u2013100.","journal-title":"Hematology"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1179\/102453311X12902908411832"},{"key":"e_1_3_3_7_1","first-page":"817","article-title":"Impaired expression of the CD3-zeta chain in peripheral blood T cells of patients with chronic myeloid leukaemia results in an increased susceptibility to apoptosis","volume":"111","author":"Chen X.","year":"2000","unstructured":"Chen X., , Woiciechowsky A., , Raffegerst S., , Schendel D., , Kolb H.J., , Roskrow M. 2000. Impaired expression of the CD3-zeta chain in peripheral blood T cells of patients with chronic myeloid leukaemia results in an increased susceptibility to apoptosis. Br J Haematol, 111:817\u2013825.","journal-title":"Br J Haematol"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501048200"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.163.1.507"},{"key":"e_1_3_3_10_1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1146\/annurev.iy.06.040188.003213","article-title":"The T cell receptor\/CD3 complex: a dynamic protein ensemble","volume":"6","author":"Clevers H.","year":"1988","unstructured":"Clevers H., , Alarcon B., , Wileman T., , Terhorst C. 1988. The T cell receptor\/CD3 complex: a dynamic protein ensemble. Annu Rev Immunol, 6:629\u2013662.","journal-title":"Annu Rev Immunol"},{"key":"e_1_3_3_11_1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.mam.2006.12.006","article-title":"Redirecting T lymphocyte specificity by T cell receptor gene transfer\u2014a new era for immunotherapy","volume":"28","author":"Engels B.","year":"2007","unstructured":"Engels B., , Uckert W. 2007. Redirecting T lymphocyte specificity by T cell receptor gene transfer\u2014a new era for immunotherapy. Mol Aspects Med, 28:115\u2013142.","journal-title":"Mol Aspects Med"},{"key":"e_1_3_3_12_1","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1097\/01.all.0000191886.12645.79","article-title":"CD3 deficiencies","volume":"5","author":"Fischer A.","year":"2005","unstructured":"Fischer A., , de Saint Basile G., , Le Deist F. 2005. CD3 deficiencies. Curr Opin Allergy Clin Immunol, 5:491\u2013495.","journal-title":"Curr Opin Allergy Clin Immunol"},{"key":"e_1_3_3_13_1","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1054\/bjoc.2001.1792","article-title":"The effect of peripheral blood lymphocyte stimulation on zeta chain expression and IL-2 production in Hodgkin's disease","volume":"84","author":"Frydecka I.","year":"2001","unstructured":"Frydecka I., , Bocko D., , Kosmaczewska A., , Ciszak L., , Morilla R. 2001. The effect of peripheral blood lymphocyte stimulation on zeta chain expression and IL-2 production in Hodgkin's disease. Br J Cancer, 84:1339\u20131343.","journal-title":"Br J Cancer"},{"key":"e_1_3_3_14_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3324\/haematol.2009.016022","article-title":"T-cell receptor gene transfer for the treatment of leukemia and other tumors","volume":"95","author":"Heemskerk M.H.","year":"2010","unstructured":"Heemskerk M.H. 2010. T-cell receptor gene transfer for the treatment of leukemia and other tumors. Haematologica, 95:15\u201319.","journal-title":"Haematologica"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/clim.2002.5192"},{"key":"e_1_3_3_16_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s11523-008-0100-y","article-title":"Treatment selection after imatinib resistance in chronic myeloid leukemia","volume":"4","author":"Jabbour E.","year":"2009","unstructured":"Jabbour E., , Cortes J., , Kantarjian H. 2009. Treatment selection after imatinib resistance in chronic myeloid leukemia. Target Oncol, 4:3\u201310.","journal-title":"Target Oncol"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1179\/102453308X343482"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1179\/102453311X12953015767491"},{"key":"e_1_3_3_19_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1179\/102453311X12902908411634","article-title":"Restricted TRBV repertoire in CD4+ and CD8+ T-cell subsets from CML patients","volume":"16","author":"Li Y.","year":"2011","unstructured":"Li Y., , Geng S., , Du X., , Chen S., , Yang L., , Wu X., , Li B., , Schmidt C.A., , Przybylski G.K. 2011b. Restricted TRBV repertoire in CD4+ and CD8+ T-cell subsets from CML patients. Hematology, 16:43\u201349.","journal-title":"Hematology"},{"key":"e_1_3_3_20_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/1479-5876-8-47","article-title":"Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients","volume":"8","author":"Li Y.","year":"2010","unstructured":"Li Y., , Geng S., , Yin Q., , Chen S., , Yang L., , Wu X., , Li B., , Du X., , Schmidt C.A., , Przybylski G.K. 2010. Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients. J Transl Med, 8:47.","journal-title":"J Transl Med"},{"key":"e_1_3_3_21_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1046\/j.1365-2141.2003.04789.x","article-title":"Signalling molecules and cytokine production in T cells of multiple myeloma-increased abnormalities with advancing stage","volume":"124","author":"Mozaffari F.","year":"2004","unstructured":"Mozaffari F., , Hansson L., , Kiaii S., , Ju X., , Rossmann E.D., , Rabbani H., , Mellstedt H., , Osterborg A. 2004. Signalling molecules and cytokine production in T cells of multiple myeloma-increased abnormalities with advancing stage. Br J Haematol, 124:315\u2013324.","journal-title":"Br J Haematol"},{"key":"e_1_3_3_22_1","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1006\/cimm.2001.1761","article-title":"Dexamethasone modulates TCR zeta chain expression and antigen receptor-mediated early signaling events in human T lymphocytes","volume":"208","author":"Nambiar M.P.","year":"2001","unstructured":"Nambiar M.P., , Enyedy E.J., , Fisher C.U., , Warke V.G., , Juang Y.T., , Tsokos G.C. 2001. Dexamethasone modulates TCR zeta chain expression and antigen receptor-mediated early signaling events in human T lymphocytes. Cell Immunol, 208:62\u201371.","journal-title":"Cell Immunol"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11072"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-144790"},{"key":"e_1_3_3_25_1","doi-asserted-by":"crossref","first-page":"e30713","DOI":"10.1371\/journal.pone.0030713","article-title":"The CD3-zeta chimeric antigen receptor overcomes TCR Hypo-responsiveness of human terminal late-stage T cells","volume":"7","author":"Rappl G.","year":"2012","unstructured":"Rappl G., , Riet T., , Awerkiew S., , Schmidt A., , Hombach A.A., , Pfister H., , Abken H. 2012. The CD3-zeta chimeric antigen receptor overcomes TCR Hypo-responsiveness of human terminal late-stage T cells. PLoS One, 7:e30713.","journal-title":"PLoS One"},{"key":"e_1_3_3_26_1","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1182\/blood.V88.1.236.236","article-title":"T cells from patients with Hodgkin's disease have a defective T-cell receptor zeta chain expression that is reversible by T-cell stimulation with CD3 and CD28","volume":"88","author":"Renner C.","year":"1996","unstructured":"Renner C., , Ohnesorge S., , Held G., , Bauer S., , Jung W., , Pfitzenmeier J.P., , Pfreundschuh M. 1996. T cells from patients with Hodgkin's disease have a defective T-cell receptor zeta chain expression that is reversible by T-cell stimulation with CD3 and CD28. Blood, 88:236\u2013241.","journal-title":"Blood"},{"key":"e_1_3_3_27_1","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1182\/blood.V94.3.1063.415a17_1063_1069","article-title":"Oligoclonal TCRBV gene usage in B-cell chronic lymphocytic leukemia: major perturbations are preferentially seen within the CD4 T-cell subset","volume":"94","author":"Rezvany M.R.","year":"1999","unstructured":"Rezvany M.R., , Jeddi-Tehrani M., , Osterborg A., , Kimby E., , Wigzell H., , Mellstedt H. 1999. Oligoclonal TCRBV gene usage in B-cell chronic lymphocytic leukemia: major perturbations are preferentially seen within the CD4 T-cell subset. Blood, 94:1063\u20131069.","journal-title":"Blood"},{"key":"e_1_3_3_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301598200"},{"key":"e_1_3_3_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2368"},{"key":"e_1_3_3_30_1","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1096\/fj.02-1100rev","article-title":"NF-kappaB activation pathways induced by T cell costimulation","volume":"17","author":"Schmitz M.L.","year":"2003","unstructured":"Schmitz M.L., , Bacher S., , Dienz O. 2003. NF-kappaB activation pathways induced by T cell costimulation. FASEB J, 17:2187\u20132193.","journal-title":"FASEB J"},{"key":"e_1_3_3_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-011-1126-z"},{"key":"e_1_3_3_32_1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/1479-5876-10-48","article-title":"New directions in cellular therapy of cancer: a summary of the summit on cellular therapy for cancer","volume":"10","author":"Stroncek D.F.","year":"2012","unstructured":"Stroncek D.F., , Berger C., , Cheever M.A., , Childs R.W., , Dudley M.E., , Flynn P., , Gattinoni L., , Heath J.R., , Kalos M., , Marincola F.M., , Miller J.S., , Mostoslavsky G., , Powell D.J.Jr., , Rao M., , Restifo N.P., , Rosenberg S.A., , O'Shea J., , Melief C.J. 2012. New directions in cellular therapy of cancer: a summary of the summit on cellular therapy for cancer. J Transl Med, 10:48.","journal-title":"J Transl Med"},{"key":"e_1_3_3_33_1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1046\/j.1365-2141.2003.04044.x","article-title":"Defective expression of the T-cell receptor-CD3 zeta chain in T-cell acute lymphoblastic leukaemia","volume":"120","author":"Torelli G.F.","year":"2003","unstructured":"Torelli G.F., , Paolini R., , Tatarelli C., , Soriani A., , Vitale A., , Guarini A., , Santoni A., , Foa R. 2003. Defective expression of the T-cell receptor-CD3 zeta chain in T-cell acute lymphoblastic leukaemia. Br J Haematol, 120:201\u2013208.","journal-title":"Br J Haematol"},{"key":"e_1_3_3_34_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/j.1365-2249.2005.02715.x","article-title":"Exploiting T cell receptor genes for cancer immunotherapy","volume":"139","author":"Xue S.","year":"2005","unstructured":"Xue S., , Gillmore R., , Downs A., , Tsallios A., , Holler A., , Gao L., , Wong V., , Morris E., , Stauss H.J. 2005. Exploiting T cell receptor genes for cancer immunotherapy. Clin Exp Immunol, 139:167\u2013172.","journal-title":"Clin Exp Immunol"},{"key":"e_1_3_3_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-8722-4-2"},{"key":"e_1_3_3_36_1","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.humimm.2011.06.015","article-title":"Characterization of the CDR3 structure of the Vbeta21 T cell clone in patients with P210(BCR-ABL)-positive chronic myeloid leukemia and B-cell acute lymphoblastic leukemia","volume":"72","author":"Zha X.","year":"2011","unstructured":"Zha X., , Chen S., , Yang L., , Li B., , Chen Y., , Yan X., , Li Y. 2011. Characterization of the CDR3 structure of the Vbeta21 T cell clone in patients with P210(BCR-ABL)-positive chronic myeloid leukemia and B-cell acute lymphoblastic leukemia. Hum Immunol, 72:798\u2013804.","journal-title":"Hum Immunol"}],"container-title":["DNA and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/dna.2012.1798","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/dna.2012.1798","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/dna.2012.1798","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T15:11:09Z","timestamp":1773241869000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/dna.2012.1798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":35,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1089\/dna.2012.1798"],"URL":"https:\/\/doi.org\/10.1089\/dna.2012.1798","relation":{},"ISSN":["1044-5498","1557-7430"],"issn-type":[{"value":"1044-5498","type":"print"},{"value":"1557-7430","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11]]}}}