{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:50:36Z","timestamp":1785217836190,"version":"3.55.0"},"reference-count":26,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2019,10,30]],"date-time":"2019-10-30T00:00:00Z","timestamp":1572393600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["DNA and Cell Biology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p>\n                    This study investigated whether overexpression of paired-related homeobox 1 (prrx1) can successfully induce differentiation of brown adipose-derived stem cells (BADSCs) into sinus node-like cells. The experiments were performed in two groups: adenovirus\u2013green fluorescent protein (Ad-GFP) group and Ad-prrx1 group. After 5\u20137 days of adenoviral transfection, the expression levels of sinus node cell-associated pacing protein (hyperpolarization-activated cyclic nucleotide-gated potassium channel 4 [HCN4]) and ion channel (calcium channel, voltage-dependent, T type, alpha 1G subunit [Cacna1g]), as well as transcription factors (T-box 18 [TBX18], insulin gene enhancer binding protein 1 [ISL-1], paired-like homeodomain transcription factor 2 [pitx2], short stature homeobox 2 [shox2]), were detected by western blot and reverse transcription\u2013quantitative polymerase chain reaction. Immunofluorescence assay was carried out to detect whether prrx1 was coexpressed with HCN4, TBX18, and ISL-1. Finally, whole-cell patch-clamp technique was used to record pacing current hyperpolarization-activated inward current (I\n                    <jats:sub>f<\/jats:sub>\n                    ). The isolated cells were CD90\n                    <jats:sup>+<\/jats:sup>\n                    , CD29\n                    <jats:sup>+<\/jats:sup>\n                    , and CD45\n                    <jats:sup>\u2212<\/jats:sup>\n                    , indicating that pure BADSCs were successfully isolated. After 5\u20137 days of Ad transfection into cells, the mRNA levels and protein levels of pacing-related factors (TBX18, ISL-1, HCN4, shox2, and Cacna1g) in Ad-prrx1 group were significantly higher than those in Ad-GFP group. However, the expression level of pitx2 was decreased. Immunofluorescence analysis showed that prrx1 was coexpressed with TBX18, ISL-1, and HCN4 in the Ad-prrx1 group, which did not appear in the Ad-GFP group. Whole-cell patch clamps were able to record the I\n                    <jats:sub>f<\/jats:sub>\n                    current in the experimental group rather than in the Ad-GFP group. Overexpression of prrx1 can successfully induce sinus node-like cells.\n                  <\/jats:p>","DOI":"10.1089\/dna.2019.4998","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T11:40:46Z","timestamp":1569238846000},"page":"1313-1322","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Transcription Factor prrx1 Promotes Brown Adipose-Derived Stem Cells Differentiation to Sinus Node-Like Cells"],"prefix":"10.1177","volume":"38","author":[{"given":"Lin","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, P.R. China."},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, P.R. China."},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, P.R. China."},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, P.R. China."},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, P.R. China."},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, P.R. China."},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, P.R. China."},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, P.R. China."},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-hong","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, P.R. China."},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, P.R. China."},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing-yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, P.R. China."},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, P.R. China."},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, P.R. China."},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, P.R. China."},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, P.R. China."},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, P.R. China."},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, P.R. China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong-xin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, P.R. China."},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, P.R. China."},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, P.R. 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(2018). The pattern of mRNA expression is changed in sinoatrial node from Goto-Kakizaki type 2 diabetic rat heart. 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(2018). Comparison of mouse brown and white adiposederived stem cell differentiation into pacemakerlike cells induced by TBX18 transduction. Mol Med Rep, 17, 7055\u20137064.","journal-title":"Mol Med Rep"},{"key":"e_1_3_4_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCGENETICS.117.001902"},{"key":"e_1_3_4_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16023071"},{"key":"e_1_3_4_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1405411111"},{"key":"e_1_3_4_27_1","first-page":"879","article-title":"Insulin gene enhancer binding protein 1 induces adipose tissue-derived stem cells to differentiate into pacemaker-like cells","volume":"43","author":"Zhang J.","year":"2019","unstructured":"Zhang J., , Yang M., , Yang A., , Wang X., , Tang Y., , Zhao Q., et al. (2019). Insulin gene enhancer binding protein 1 induces adipose tissue-derived stem cells to differentiate into pacemaker-like cells. 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