{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:53:39Z","timestamp":1792115619014,"version":"4.4.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["DNA and Cell Biology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:p>\n                    Serine protease inhibitor b5 (SERPINB5) is a tumor suppressor gene that plays a critical role in various cellular processes. In gallbladder cancer (GBC), SERPINB5's aberrant expression is reported but its role in genetic predisposition is not known. We enrolled 270 cases and 296 controls and genotyped them for single nucleotide polymorphisms (SNPs) using direct DNA sequencing, followed by genotype\u2013phenotype analysis in GBC and other cancer cell lines. Luciferase assay was done to determine the role of rs2289521 SNP on expression regulation. We found that two SERPINB5 variants rs2289519 and rs2289521 are significantly associated with GBC and contribute to genetic predisposition. The TT genotype of variant rs2289519 was found to be significantly associated (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.008) with GBC in a recessive model. C allele of rs2289521 increased the risk for GBC significantly at genotypic (CT,\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.026) and allelic (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.04) levels.\n                    <jats:italic toggle=\"yes\">In silico<\/jats:italic>\n                    analysis and luciferase assay uncovered the probable regulatory role of the rs2289521 variant on expression. Genotype\u2013phenotype correlation in GBC and breast cancer cell lines showed reduced expression of SERPINB5 in the presence of C allele that was consistent with the result of luciferase assay. Overall, our study reveals the genetic association of two SERPINB5 variants with GBC and rs2289521's possible role in the regulation of expression.\n                  <\/jats:p>","DOI":"10.1089\/dna.2021.0056","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T23:07:16Z","timestamp":1615417636000},"page":"706-712","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["<i>SERPINB5<\/i>\n                    Genetic Variants rs2289519 and rs2289521 are Significantly Associated with Gallbladder Cancer Risk"],"prefix":"10.1177","volume":"40","author":[{"given":"Biswaheree","family":"Mahananda","sequence":"first","affiliation":[{"name":"School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India."},{"name":"Homi Bhabha National Institute, Mumbai, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Vinay","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India."},{"name":"Homi Bhabha National Institute, Mumbai, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananya","family":"Palo","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India."},{"name":"Homi Bhabha National Institute, Mumbai, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaskanta","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepato-Biliary Sciences, IMS &amp; SUM Hospital, Sikshya O Anusandhan University, Bhubaneswar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saroj Kanta","family":"Sahu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepato-Biliary Sciences, IMS &amp; SUM Hospital, Sikshya O Anusandhan University, Bhubaneswar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivaram Prasad","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Sriram Chandra Bhanja Medical College and Hospital, Cuttack, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manjusha","family":"Dixit","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, National Institute of Science Education and Research, Bhubaneswar, India."},{"name":"Homi Bhabha National Institute, Mumbai, India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,5,3]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000040245.70349.37"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6791"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajco.13129"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20782"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bth457"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1331360100"},{"key":"e_1_3_4_8_1","first-page":"24","article-title":"Epidemiology of digestive tract cancers in India, I.V","volume":"18","author":"Dhir V.","year":"1999","unstructured":"Dhir V., , and Mohandas K.M. (1999). Epidemiology of digestive tract cancers in India, I.V. Gall bladder and pancreas. Indian J Gastroenterol, 18, 24\u201328.","journal-title":"Gall bladder and pancreas. Indian J Gastroenterol"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40164-015-0024-z"},{"key":"e_1_3_4_10_1","first-page":"60","article-title":"BioEdit: an important software for molecular biology","volume":"2","author":"Hall T.A.","year":"2011","unstructured":"Hall T.A. (2011). BioEdit: an important software for molecular biology. 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