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In this study, the binding interaction of Harmane (a naturally occurring, bioactive indole alkaloid) with two natural polymeric DNAs, that is, Calf thymus (CT) DNA and Herring testis (HT) DNA has been elucidated using biophysical techniques. A ground state, 1:1 complexation, was revealed by steady-state fluorescence spectroscopy. The thermodynamic profile and energetics of the associated reaction were evaluated by temperature-dependent fluorescence spectroscopy.<\/jats:p>\n                  <jats:p>\n                    The spontaneity of the binding was confirmed by the negative \u0394\n                    <jats:italic toggle=\"yes\">G<\/jats:italic>\n                    \u00b0 values in both cases. Negative enthalpy change, along with stronger positive entropic contribution, indicated the dominant electrostatic nature of the interaction and finally the entropy-driven exothermic binding process throughout. Salt-dependent studies further demonstrated the significant contribution of electrostatic interactions in ligand binding toward DNA. Infrared data substantiated the structural information of the said interactions, leading to the exploration of the structure\u2013function relationship.\n                  <\/jats:p>","DOI":"10.1089\/dna.2021.0132","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T09:08:27Z","timestamp":1642669707000},"page":"91-102","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Targeting Natural Polymeric DNAs with Harmane: An Insight into Binding and Thermodynamic Interaction Through Biophysical Approach"],"prefix":"10.1177","volume":"41","author":[{"given":"Soching","family":"Luikham","sequence":"first","affiliation":[{"name":"Department of Chemistry, National Institute of Technology Nagaland, Chumukedima, Dimapur, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1278-3431","authenticated-orcid":false,"given":"Jhimli","family":"Bhattacharyya","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Institute of Technology Nagaland, Chumukedima, Dimapur, India."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,2,9]]},"reference":[{"key":"e_1_3_4_2_1","first-page":"2197","article-title":"Interaction of doxorubicin with polynucleotides","volume":"53","author":"Airoldi M.","year":"2014","unstructured":"Airoldi M., , Barone G., , Gennaro G., , Giuliani A.M., , and Giustini M. 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