{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:42:54Z","timestamp":1777552974434,"version":"3.51.4"},"reference-count":60,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["DNA and Cell Biology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>\n                    The male sex-determining gene, sex-determining region on the Y chromosome (\n                    <jats:italic toggle=\"yes\">SRY<\/jats:italic>\n                    ), is expressed in adult testicular germ cells; however, its role in regulating spermatogenesis remains unclear. The role of\n                    <jats:italic toggle=\"yes\">SRY<\/jats:italic>\n                    in the postmeiotic gene expression was investigated by determining the effect of\n                    <jats:italic toggle=\"yes\">SRY<\/jats:italic>\n                    on the promoter of the haploid-specific Protamine 1 (\n                    <jats:italic toggle=\"yes\">PRM1<\/jats:italic>\n                    ) gene, which harbors five distinct\n                    <jats:italic toggle=\"yes\">SRY<\/jats:italic>\n                    -binding motifs. In a luciferase reporter assay system,\n                    <jats:italic toggle=\"yes\">SRY<\/jats:italic>\n                    upregulates\n                    <jats:italic toggle=\"yes\">PRM1<\/jats:italic>\n                    promoter activity\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    in a dose-dependent manner. Through a gel-shift assay involving a 31-bp DNA fragment encompassing the\n                    <jats:italic toggle=\"yes\">SRY<\/jats:italic>\n                    element within the\n                    <jats:italic toggle=\"yes\">PRM1<\/jats:italic>\n                    promoter, the third\n                    <jats:italic toggle=\"yes\">SRY<\/jats:italic>\n                    -binding site on the sense strand (\u2212373\/\u2212367) was identified as crucial for\n                    <jats:italic toggle=\"yes\">PRM1<\/jats:italic>\n                    promoter activation. This assay was extended to analyze 9\n                    <jats:italic toggle=\"yes\">SRY<\/jats:italic>\n                    variants found in the testicular DNA of 44 azoospermia patients. The findings suggest that\n                    <jats:italic toggle=\"yes\">SRY<\/jats:italic>\n                    regulates\n                    <jats:italic toggle=\"yes\">PRM1<\/jats:italic>\n                    promoter activity by directly binding to its specific motif within the\n                    <jats:italic toggle=\"yes\">PRM1<\/jats:italic>\n                    promoter.\n                  <\/jats:p>","DOI":"10.1089\/dna.2022.0619","type":"journal-article","created":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T10:37:11Z","timestamp":1704278231000},"page":"12-25","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular and Functional Characterization of Human Sex-Determining Region on the Y Chromosome Variants Using Protamine 1 Promoter"],"prefix":"10.1177","volume":"43","author":[{"given":"Deepali","family":"Pathak","sequence":"first","affiliation":[{"name":"School of Sciences, Jain (Deemed-to-Be University), Bengaluru, Karnataka, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arka","family":"Baksi","sequence":"additional","affiliation":[{"name":"Institute of Physiological Chemistry, Faculty of Medicine, University Hospital Carl Gustav Carus, TU-Dresden, Saxony, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.S.","family":"Vasan","sequence":"additional","affiliation":[{"name":"Manipal Ankur Fertility, Bengaluru, Karnataka, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1698-3657","authenticated-orcid":false,"given":"Rajan R.","family":"Dighe","sequence":"additional","affiliation":[{"name":"Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru, Karnataka, India."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,1,12]]},"reference":[{"key":"e_1_3_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-002-0376-9"},{"key":"e_1_3_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03347266"},{"key":"e_1_3_6_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/394049"},{"key":"e_1_3_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29369-8"},{"key":"e_1_3_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-9-227"},{"key":"e_1_3_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/348448a0"},{"key":"e_1_3_6_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043380"},{"key":"e_1_3_6_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9883"},{"key":"e_1_3_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1997)9:5<388::AID-HUMU2>3.0.CO;2-0"},{"key":"e_1_3_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/2.12.2007"},{"key":"e_1_3_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-018-0330-5"},{"key":"e_1_3_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2012.07782.x"},{"key":"e_1_3_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.01.005"},{"key":"e_1_3_6_15_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.2007-0250"},{"key":"e_1_3_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.1991.tb02575.x"},{"key":"e_1_3_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150100515"},{"key":"e_1_3_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejhg.5201719"},{"key":"e_1_3_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/346245a0"},{"key":"e_1_3_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12330"},{"key":"e_1_3_6_21_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.2002-0025"},{"key":"e_1_3_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2013.04.027"},{"key":"e_1_3_6_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1995.1252"},{"key":"e_1_3_6_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040858"},{"key":"e_1_3_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(97)81365-9"},{"key":"e_1_3_6_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-0226"},{"key":"e_1_3_6_27_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.87.7.8646"},{"key":"e_1_3_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb763"},{"key":"e_1_3_6_29_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.048983"},{"key":"e_1_3_6_30_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20091758"},{"issue":"3","key":"e_1_3_6_31_1","first-page":"307","article-title":"Y chromosome microdeletions in infertile men with non-obstructive azoospermia and severe oligozoospermia","volume":"18","author":"Kim SY","year":"2017","unstructured":"Kim SY, , Kim HJ, , Lee BY, et al. 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