{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:46:51Z","timestamp":1793148411076,"version":"4.5.0"},"reference-count":29,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["DNA and Cell Biology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:p>\n                    Although the contribution of ferroptosis, an iron-dependent cell death, to ischemia reperfusion (IR)-induced retinal injury has been reported before, to optimize therapeutic strategy, there is still an urgent need to identify potential candidates involved in this process. Androgen Receptor-Associated Protein of 70\u2009kDa (ARA70) is a cargo receptor for ferritinophagy, and its role in retinal ferroptosis has not been revealed yet. Herein, we explored the role of ARA70 in IR-associated retinal lesions by\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    (C57BL\/6\u2009J mice with intraocular pressure of 90\u2013100\u2009mmHg) and\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    (retinal ganglion cells (RGCs) stimulated with tert-butyl hydroperoxide (tBH)) experiments. It was found that IR upregulated ARA70 expression and accelerated lipid peroxidation in retinal tissues. We first confirmed that two ferroptosis inhibitors, deferiprone or ferrostatin-1 (Fer-1), suppressed ferritin degradation, restrained apoptosis and inflammation, and protected mouse retinas against IR stress. Next, primary mouse RGCs were treated with tBH to simulate IR environment\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    . ARA70 expression was decreased at lower concentrations of tBH (5\u201320\u2009\u03bcM), but increased at higher concentrations (40\u201380\u2009\u03bcM). Interestingly, the expression of ferritin-related proteins (ferritin heavy chain, FTH; ferritin light chain, FTL) showed an opposite alteration. Knockdown of ARA70 protected RGCs from tBH-induced damage. It inhibited the delivery of ferritin to lysosomes for ferritinophagy and thus reducing cellular Fe\n                    <jats:sup>2+<\/jats:sup>\n                    concentration. Besides, ARA70 knockdown suppressed autophagy and inflammation of tBH-treated RGCs. These findings provide novel insights into the pathogenesis of retinal IR, and may be helpful for treatment of retinal diseases.\n                  <\/jats:p>","DOI":"10.1089\/dna.2023.0077","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T14:37:05Z","timestamp":1698676625000},"page":"668-679","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["A Novel Role of ARA70 in Regulating Ferritinophagy of RGCs During Retinal Ischemia Reperfusion"],"prefix":"10.1177","volume":"42","author":[{"given":"Xin","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nannan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanqi","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4471-5679","authenticated-orcid":false,"given":"Yongsheng","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,11,10]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2019.232124"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.042"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(03)00050-3"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-7793"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.06.011"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2015.06.001"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-6113"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2016.1187366"},{"key":"e_1_3_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394309-5.00006-7"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-48087-3"},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-022-02621-9"},{"key":"e_1_3_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13148"},{"key":"e_1_3_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.optom.2017.06.002"},{"key":"e_1_3_5_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00444"},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2003.12.001"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300752r"},{"key":"e_1_3_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04911-9"},{"key":"e_1_3_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-04911-9"},{"key":"e_1_3_5_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9NR07171G"},{"key":"e_1_3_5_21_1","first-page":"9","article-title":"Rapamycin and fasting sustain autophagy response activated by ischemia\/reperfusion injury and promote retinal ganglion cell survival","author":"Russo R","year":"2018","unstructured":"Russo R, , Varano GP, , Adornetto A, et al. Rapamycin and fasting sustain autophagy response activated by ischemia\/reperfusion injury and promote retinal ganglion cell survival. Cell Death Dis, 2018; 9.","journal-title":"Cell Death Dis"},{"key":"e_1_3_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-022-01025-1"},{"key":"e_1_3_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.12.010"},{"key":"e_1_3_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2008.02.010"},{"key":"e_1_3_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.10.014"},{"key":"e_1_3_5_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-022-01046-4"},{"key":"e_1_3_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-022-03697-8"},{"key":"e_1_3_5_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9929687"},{"key":"e_1_3_5_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2022.928321"},{"key":"e_1_3_5_30_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9961628"}],"container-title":["DNA and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/dna.2023.0077","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/dna.2023.0077","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/dna.2023.0077","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T17:59:54Z","timestamp":1777399194000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/dna.2023.0077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11]]},"references-count":29,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2023,11]]}},"alternative-id":["10.1089\/dna.2023.0077"],"URL":"https:\/\/doi.org\/10.1089\/dna.2023.0077","relation":{},"ISSN":["1044-5498","1557-7430"],"issn-type":[{"value":"1044-5498","type":"print"},{"value":"1557-7430","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11]]}}}