{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T13:47:25Z","timestamp":1784814445515,"version":"3.55.0"},"reference-count":49,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["DNA and Cell Biology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:p>\n                    Multiple myeloma (MM) is a hematologic malignancy that results from uncontrolled plasma cell proliferation. Circular RNAs are versatile regulators that influence cancer aggression. The pathogenic mechanism of circXPO1 in MM is still unknown. In this study, the expression of circXPO1, miR-495-3p, and DNA damage-induced transcription 4 (\n                    <jats:italic toggle=\"yes\">DDIT4<\/jats:italic>\n                    ) was detected. Knockdown and overexpression assays were used to evaluate the effect of circXPO1 on MM. Specifically, 5-ethynyl-2\u2032-deoxyuridine and cell counting kit-8 assay were used to investigate cell proliferation. Meanwhile, flow cytometry was adopted to detect cell apoptosis and cell cycle. Apoptosis-associated and cell cycle-related proteins were detected by Western blot. Mechanistically, biotin RNA pull-down assay and dual-luciferase assay were implemented to verify the combination among miR495-3p and circXPO1 or\n                    <jats:italic toggle=\"yes\">DDIT4<\/jats:italic>\n                    . The function of circXPO1\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    was explored in xenograft experiments. The results showed that circXPO1 was up-regulated in both MM samples and MM cell lines and miR-495-3p was down-regulated in MM patients. Silencing circXPO1 inhibited cell proliferation, increased apoptosis rates, and caused the G1 phase arrest. Overexpression of circXPO1 yielded opposite results. In addition, RNA pull-down experiment demonstrated the interaction between circXPO1 and miR-495-3p. Silencing miR-495-3p rescued the inhibitory function caused by the knockdown of circXPO1.\n                    <jats:italic toggle=\"yes\">DDIT4<\/jats:italic>\n                    was the target of miR-495-3p. Finally, silencing circXPO1 inhibited the growth of subcutaneous tumors\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    . In conclusion, our findings showed that circXPO1 could promote MM progression via the miR-495-3p\/\n                    <jats:italic toggle=\"yes\">DDIT4<\/jats:italic>\n                    axis.\n                  <\/jats:p>","DOI":"10.1089\/dna.2023.0288","type":"journal-article","created":{"date-parts":[[2023,12,11]],"date-time":"2023-12-11T12:04:34Z","timestamp":1702296274000},"page":"39-55","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Circular RNA circXPO1 Promotes Multiple Myeloma Progression by Regulating miR-495-3p\/DNA Damage-Induced Transcription 4 Axis"],"prefix":"10.1177","volume":"43","author":[{"given":"Fangmei","family":"Li","sequence":"first","affiliation":[{"name":"Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyu","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5229-829X","authenticated-orcid":false,"given":"Rui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0895-7750","authenticated-orcid":false,"given":"Yun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2521-3806","authenticated-orcid":false,"given":"Aili","family":"He","sequence":"additional","affiliation":[{"name":"Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianli","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,1,12]]},"reference":[{"key":"e_1_3_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-019-0205-9"},{"key":"e_1_3_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00687-1"},{"key":"e_1_3_7_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.23.090506.123406"},{"key":"e_1_3_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.712145"},{"key":"e_1_3_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz757"},{"key":"e_1_3_7_7_1","first-page":"3912","article-title":"Chromatin isolation by RNA purification (ChIRP)","volume":"61","author":"Chu C","year":"2012","unstructured":"Chu C, , Quinn J, , Chang HY. 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