{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T22:36:39Z","timestamp":1777588599777,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Endourology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction:<\/jats:title>\n                    <jats:p>The creation of synthetic reservoirs for bladder replacement has been limited by challenges of interfacing synthetic materials and native tissue. We sought to overcome this challenge by utilizing a novel bilayer silk fibroin scaffold (BLSF) as an intermediary toward the development of an acellular prosthetic reservoir.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>\n                      Under institutionally approved protocols, 3D-printed reservoirs were implanted in six juvenile female pigs after cystectomy. BLSF was attached to the\n                      <jats:italic toggle=\"yes\">in situ<\/jats:italic>\n                      prosthetic reservoir serving as an intermediary to native ureteral and urethral tissue anastomoses. Our first protocol allowed four pigs to be survived up to 7 days, and the second protocol allowed two pigs to be survived for up to 1 year. At the first sign of functional decline or the end of the study period, the animals were euthanized, and kidneys, ureters, prosthetic bladder, and urethra were harvested en bloc for histopathology analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The first two pigs had anastomotic urine leaks because of design flaws resulting in early termination. The third pig had acute renal failure resulting in early termination. The artificial bladder design was modified in subsequent iterations. The fourth pig survived for 7 days and, upon autopsy, had intact urethral and ureteral anastomoses. The fifth and sixth pigs survived for 11 and 12 weeks, respectively, before they were sacrificed because of failure to thrive. One animal developed an enteric fistula. The other animal had an intact anastomosis, and the BLFS was identified at the ureteral and urethral anastomoses on histopathologic analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Replacing the porcine bladder with a prosthetic bladder was achieved for up to 3 months, the second longest survival period for a nonbiologic bladder alternative. BLSF was used for the first time to create an interface between synthetic material and biologic tissue by allowing ingrowth of urothelium onto the acellular alloplastic bladder.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/end.2022.0296","type":"journal-article","created":{"date-parts":[[2022,10,27]],"date-time":"2022-10-27T13:45:26Z","timestamp":1666878326000},"page":"341-352","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Porcine Bladder Replacement with a Bilayer Silk Fibroin Enhanced Prosthetic Reservoir: A Feasibility Study"],"prefix":"10.1177","volume":"37","author":[{"given":"Pengbo","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sohrab N.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphael B.","family":"Arada","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1368-8786","authenticated-orcid":false,"given":"Akhil","family":"Peta","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Brevik","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maged","family":"Ayad","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalon L.","family":"Morgan","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krista","family":"Larson","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Larson","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gokhan","family":"Gundogdu","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shlomi","family":"Tapiero","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ted","family":"Farzaneh","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roshan M.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua","family":"Mauney","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime","family":"Landman","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph V.","family":"Clayman","sequence":"additional","affiliation":[{"name":"Department of Urology and University of California, Irvine, California, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2023,3,1]]},"reference":[{"issue":"1","key":"e_1_3_4_2_2","first-page":"E15","article-title":"Epidemiology of bladder cancer","volume":"8","author":"Saginala K","year":"2020","unstructured":"Saginala K, , Barsouk A, , Aluru JS, et al. Epidemiology of bladder cancer. Med Sci (Basel), 2020; 8(1):E15.","journal-title":"Med Sci (Basel)"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-19199-z"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2020.03.055"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2019.09.035"},{"key":"e_1_3_4_6_2","unstructured":"Benchmark Reports\u2014Urinary bladder cancer. In: National Cancer Database (NCDB): American College of Surgeons; 2020. https:\/\/www.facs.org\/quality-programs\/cancer-programs\/national-cancer-database\/publicaccess\/ [Last accessed: January 7 2022]."},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-012-0249-2"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2010.05.037"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2001.19.3.666"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ucl.2015.01.005"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.121331"},{"key":"e_1_3_4_12_2","first-page":"900","article-title":"Replacement of the urinary bladder by a plastic reservoir with mechanical valves","volume":"10","author":"Bogash M","year":"1960","unstructured":"Bogash M, , Kohler FP, , Scott RH, et al. Replacement of the urinary bladder by a plastic reservoir with mechanical valves. Surg Forum, 1960; 10:900\u2013903.","journal-title":"Surg Forum"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)65442-9"},{"key":"e_1_3_4_14_2","first-page":"885","article-title":"The artificial urinary bladder","volume":"539","author":"Rohrmann D","year":"2003","unstructured":"Rohrmann D. The artificial urinary bladder. Adv Exp Med Biol, 2003; 539(Pt B):885\u2013893.","journal-title":"Adv Exp Med Biol"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(12)60095-0"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002480-197700230-00095"},{"key":"e_1_3_4_17_2","first-page":"323","article-title":"Prosthetic bladder of silicone rubber in dogs","volume":"1","author":"Friedman B","year":"1964","unstructured":"Friedman B, , Smith DR, , Finkle AL. Prosthetic bladder of silicone rubber in dogs. Invest Urol, 1964; 1:323\u2013338.","journal-title":"Invest Urol"},{"issue":"3","key":"e_1_3_4_18_2","first-page":"M429","article-title":"Experimental development of a fixed volume, gravity draining, prosthetic urinary bladder","volume":"36","author":"Gleeson MJ","year":"1990","unstructured":"Gleeson MJ, , Anderson S, , Homsy C, et al. Experimental development of a fixed volume, gravity draining, prosthetic urinary bladder. ASAIO Trans, 1990; 36(3):M429\u2013M432.","journal-title":"ASAIO Trans"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01836344"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)35086-3"},{"issue":"1","key":"e_1_3_4_21_2","first-page":"1","article-title":"[Total substitution of the bladder with a prosthesis. Experimental research. Preliminary results]","volume":"28","author":"Rigotti E","year":"1976","unstructured":"Rigotti E, , Randone D, , Tizzani A, et al. [Total substitution of the bladder with a prosthesis. Experimental research. Preliminary results]. Minerva Urol, 1976; 28(1):1\u20135.","journal-title":"Minerva Urol"},{"key":"e_1_3_4_22_2","first-page":"75","article-title":"Experimental and clinical studies of prosthetic bladder replacement. Genito-Urinary Reconstruction with Prostheses","author":"Apoil A","year":"1981","unstructured":"Apoil A, , Granger A, , Sausse A, et al. Experimental and clinical studies of prosthetic bladder replacement. Genito-Urinary Reconstruction with Prostheses. Stuttgart, Thieme, 1981; 75\u201380.","journal-title":"Stuttgart, Thieme"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11934-015-0567-x"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajur.2017.06.010"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.03.038"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2016.06.014"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2018.00181"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042307-200005000-00003"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)64450-3"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)67701-4"}],"container-title":["Journal of Endourology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/end.2022.0296","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/end.2022.0296","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/end.2022.0296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T20:14:49Z","timestamp":1777407289000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/end.2022.0296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1089\/end.2022.0296"],"URL":"https:\/\/doi.org\/10.1089\/end.2022.0296","relation":{},"ISSN":["0892-7790","1557-900X"],"issn-type":[{"value":"0892-7790","type":"print"},{"value":"1557-900X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3]]}}}