{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:44:03Z","timestamp":1789656243211,"version":"4.0.1"},"reference-count":38,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:p>\n                    The precise detection of\n                    <jats:italic toggle=\"yes\">Toxoplasma gondii<\/jats:italic>\n                    infection in cattle has important public health significance. In the present study, recombinant granule antigen protein GRA7 was evaluated as a potential diagnostic marker for\n                    <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                    infection in cattle by an indirect enzyme-linked immunosorbent assay (ELISA) using the reference serum samples determined by immunofluorescence assay and Western blotting, showing that GRA7-ELISA has a sensitivity of 96.4%, and a specificity of 98.6%. The detection results by GRA7- and\n                    <jats:italic toggle=\"yes\">Toxoplasma<\/jats:italic>\n                    lysate antigen\u2013based ELISAs were compared, indicating that no significant difference (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    &gt;0.05) and perfect agreement (\u03ba=0.74) was observed between the two tests. Receiver operating characteristic analysis showed a relative sensitivity and specificity of 100% and 97.3% at the cut-off value of 0.3 for GRA7-ELISA. These data demonstrate that GRA7 is a promising serodiagnostic marker for\n                    <jats:italic toggle=\"yes\">T. gondii<\/jats:italic>\n                    infection in cattle.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2014.1749","type":"journal-article","created":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T16:25:20Z","timestamp":1406132720000},"page":"734-739","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":20,"title":["Production and Evaluation of Recombinant Granule Antigen Protein GRA7 for Serodiagnosis of\n                    <i>Toxoplasma gondii<\/i>\n                    Infection in Cattle"],"prefix":"10.1177","volume":"11","author":[{"given":"Zedong","family":"Wang","sequence":"first","affiliation":[{"name":"Military Veterinary Institute","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Ge","sequence":"additional","affiliation":[{"name":"Military Veterinary Institute","place":["China"]},{"name":"Jilin Agricultural University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiping","family":"Li","sequence":"additional","affiliation":[{"name":"Military Veterinary Institute","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxing","family":"Song","sequence":"additional","affiliation":[{"name":"Northeast Agricultural University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongchao","family":"Sun","sequence":"additional","affiliation":[{"name":"Military Veterinary Institute","place":["China"]},{"name":"Northeast Agricultural University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wei","sequence":"additional","affiliation":[{"name":"Jilin Agricultural University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Liu","sequence":"additional","affiliation":[{"name":"Military Veterinary Institute","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,9,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1144-1150.2000"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.02.003"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2910.1"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2012.1311"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(86)90106-8"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00227-2"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2013.1516"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5877(00)00115-X"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00604-12"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.09.012"},{"key":"e_1_3_3_12_1","first-page":"457","article-title":"Application of recombinant antigens in serodiagnosis of swine toxoplasmosis and prevalence of Toxoplasma gondii infection among pigs in Poland","volume":"13","author":"Holec-Gasior L","year":"2010","unstructured":"Holec-Gasior L, , Kur J, , Hiszczynska-Sawicka E, , Drapala D, , Dominiak-Gorski B, , Pejsak Z. 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