{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T13:26:58Z","timestamp":1777642018302,"version":"3.51.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>\n                    Typhoid fever caused by\n                    <jats:italic toggle=\"yes\">Salmonella enterica<\/jats:italic>\n                    serovar Typhi remains a significant public health problem in developing countries. Although the main method for diagnosing typhoid fever is blood culture, the test is time consuming and not always able to detect infections. Thus, it is very difficult to distinguish typhoid from other infections in patients with nonspecific symptoms. A simple and sensitive laboratory detection method remains necessary. The purpose of this study is to establish and evaluate a rapid and sensitive reverse transcription\u2013based loop-mediated isothermal amplification (RT-LAMP) method to detect\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    Typhi infection. In this study, a new specific gene marker, STY1607, was selected to develop a STY1607-RT-LAMP assay; this is the first report of specific RT-LAMP detection assay for typhoid. Human-simulated and clinical blood\/stool samples were used to evaluate the performance of STY1607-RT-LAMP for RNA detection; this method was compared with STY1607-LAMP, reverse transcription real-time polymerase chain reaction (rRT-PCR), and bacterial culture methods for\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    Typhi detection. Using mRNA as the template, STY1607-RT-LAMP exhibited 50-fold greater sensitivity than STY1607-LAMP for DNA detection. The STY1607-RT-LAMP detection limit is 3 colony-forming units (CFU)\/mL for both the pure\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    Typhi samples and\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    Typhi\u2013simulated blood samples and was 30 CFU\/g for the simulated stool samples, all of which were 10-fold more sensitive than the rRT-PCR method. RT-LAMP exhibited improved\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    Typhi detection sensitivity compared to culture methods and to rRT-PCR of clinical blood and stool specimens from suspected typhoid fever patients. Because it can be performed without sophisticated equipment or skilled personnel, RT-LAMP is a valuable tool for clinical laboratories in developing countries. This method can be applied in the clinical diagnosis and care of typhoid fever patients as well as for a quick public health response.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2015.1950","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T16:22:40Z","timestamp":1439482960000},"page":"778-786","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["Rapid and Sensitive\n                    <i>Salmonella<\/i>\n                    Typhi Detection in Blood and Fecal Samples Using Reverse Transcription Loop-Mediated Isothermal Amplification"],"prefix":"10.1177","volume":"12","author":[{"given":"Fenxia","family":"Fan","sequence":"first","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention","place":["China"]},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiying","family":"Yan","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention","place":["China"]},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengcheng","family":"Du","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biao","family":"Kan","sequence":"additional","affiliation":[{"name":"National Institute for Communicable Disease Control and Prevention","place":["China"]},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,9,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-7-653"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.781-788.2000"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02352-06"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.06.024"},{"key":"e_1_3_3_6_1","first-page":"325","article-title":"Development of RT-LAMP technique to detect Salmonella Typhi pathogen in blood","volume":"27","author":"Fan F","year":"2012","unstructured":"Fan F, , Wang S, , Lou J, , Chen J, , Nie Y, , Kan B, , Yan M. Development of RT-LAMP technique to detect Salmonella Typhi pathogen in blood. 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