{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:07:55Z","timestamp":1790820475616,"version":"4.1.0"},"reference-count":28,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:p>\n                    On August 28, 2015, a staphylococcal food poisoning outbreak occurred in Umbria, Italy, affecting 24 of the 42 customers who had dinner at a local restaurant. About 3\u2009h after ingesting a variety of foods, the customers manifested gastrointestinal symptoms. Within 24\u2009h of notification from the hospital emergency department, Sanitary Inspectors of the local Public Health Unit performed an epidemiological investigation. A retrospective cohort study was conducted among the customers. Food and environmental samples were collected. Due to the rapid onset of symptoms (vomiting, diarrhea), the food samples were analyzed for the presence of toxigenic bacteria and their toxins; nasopharyngeal swabs were collected from the waiters and cooks. Among the food tested, high levels of coagulase-positive staphylococci (CPS) (3.4\u2009\u00d7\u200910\n                    <jats:sup>8<\/jats:sup>\n                    CFU\/g) and staphylococcal enterotoxins (2.12\u2009ng SEA\/g) were only detected in the Chantilly cream dessert. CPS were also detected on the surface of a kitchen table (10\u2009CFU\/swab), and five food handlers were positive for\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    . In total, five enterotoxigenic\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    isolates were recovered from three food handlers, a kitchen surface, and the Chantilly cream dessert. These isolates were further characterized by biotyping, pulsed-field gel electrophoresis, and multiplex polymerase chain reaction assays for the detection of eleven enterotoxin encoding genes (\n                    <jats:italic toggle=\"yes\">sea<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">seb<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">sec<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">sed<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">see<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">seg<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">seh<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">sei<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">sej<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">sep<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">ser<\/jats:italic>\n                    ) and three genes involved in antibiotic resistance (\n                    <jats:italic toggle=\"yes\">mecA<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">mecC<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">mupA<\/jats:italic>\n                    ). Three\n                    <jats:italic toggle=\"yes\">sea<\/jats:italic>\n                    -positive strains, isolated from the dessert, environment, and one of the cooks, had the same pulsed-field gel electrophoresis profile and belonged to the human biotype, suggesting that the contamination causing the outbreak most likely originated from a food handler. Moreover, improper storage of the dessert, at room temperature for about 5\u2009h, permitted microbial growth and SEA production. This study underlines the importance of both laboratory evidence and epidemiological data for outbreak investigation.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2016.2267","type":"journal-article","created":{"date-parts":[[2017,4,12]],"date-time":"2017-04-12T14:31:30Z","timestamp":1492007490000},"page":"407-413","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":52,"title":["Investigation of a Staphylococcal Food Poisoning Outbreak from a Chantilly Cream Dessert, in Umbria (Italy)"],"prefix":"10.1177","volume":"14","author":[{"given":"Laura","family":"Ercoli","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale Umbria e Marche","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Gallina","sequence":"additional","affiliation":[{"name":"National Reference Laboratory Stafilococchi coagulasi positivi\u2014Istituto Zooprofilattico Sperimentale del Piemonte","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yacine","family":"Nia","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Est","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Auvray","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Est","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Primavilla","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale Umbria e Marche","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizia","family":"Guidi","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale Umbria e Marche","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedetta","family":"Pierucci","sequence":"additional","affiliation":[{"name":"Dipartimento Prevenzione Servizio Igiene Alimenti e Nutrizione, USL Umbria 1, Piazza Giovanni XXIII, Citta' di Castello, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catia","family":"Graziotti","sequence":"additional","affiliation":[{"name":"Dipartimento Prevenzione Servizio Igiene Alimenti e Nutrizione, USL Umbria 1, Piazza Giovanni XXIII, Citta' di Castello, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Decastelli","sequence":"additional","affiliation":[{"name":"National Reference Laboratory Stafilococchi coagulasi positivi\u2014Istituto Zooprofilattico Sperimentale del Piemonte","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Scuota","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale Umbria e Marche","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,7,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins2071751"},{"key":"e_1_3_3_3_1","unstructured":"De Buyser M-L Grout J Brisabois A Assere A Lombard B. 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