{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T09:57:01Z","timestamp":1782467821366,"version":"3.54.5"},"reference-count":27,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2017,10]]},"abstract":"\n Salmonella enterica<\/jats:italic>\n serovars Enteritidis and Typhimurium are the most common causative agents of human nontyphoidal salmonellosis. The rapid detection and timely treatment of salmonellosis are important to increase the curative ratio and prevent spreading of the disease. In this study, we developed a rapid multiplex convection polymerase chain reaction (PCR) method to detect\n Salmonella<\/jats:italic>\n spp. and differentiate\n Salmonella<\/jats:italic>\n Enteritidis and\n Salmonella<\/jats:italic>\n Typhimurium. We used the\n invA<\/jats:italic>\n gene for\n Salmonella<\/jats:italic>\n spp. detection.\n Salmonella<\/jats:italic>\n Enteritidis-specific primers and\n Salmonella<\/jats:italic>\n Typhimurium-specific primers were designed using the insertion element (\n IE<\/jats:italic>\n ) and\n spy<\/jats:italic>\n genes, respectively. The primer set for\n Salmonella<\/jats:italic>\n spp. detection clearly detected both\n Salmonella<\/jats:italic>\n Enteritidis and\n Salmonella<\/jats:italic>\n Typhimurium after a 21-min amplification reaction. Serovar-specific primer sets for\n Salmonella<\/jats:italic>\n Enteritidis and\n Salmonella<\/jats:italic>\n Typhimurium specifically detected each target species in a 21-min amplification reaction. We were able to detect\n Salmonella<\/jats:italic>\n spp. at a single copy level in the singleplex mode. The limits of detection for\n Salmonella<\/jats:italic>\n Enteritidis and\n Salmonella<\/jats:italic>\n Typhimurium were 30 copies in both the singleplex and multiplex modes. The PCR run time could be reduced to 10.5\u2009min\/15 cycles. The multiplex convection PCR method developed in this study could detect the\n Salmonella<\/jats:italic>\n spp.\n Salmonella<\/jats:italic>\n Enteritidis and\n Salmonella<\/jats:italic>\n Typhimurium in artificially contaminated milk with as few as 10\n 0<\/jats:sup>\n colony-forming unit\/mL after 4-h enrichment. The PCR assay developed in this study provides a rapid, specific, and sensitive method for the detection of\n Salmonella<\/jats:italic>\n spp. and the differentiation of\n Salmonella<\/jats:italic>\n Enteritidis and\n Salmonella<\/jats:italic>\n Typhimurium.\n <\/jats:p>","DOI":"10.1089\/fpd.2017.2290","type":"journal-article","created":{"date-parts":[[2017,7,11]],"date-time":"2017-07-11T15:56:47Z","timestamp":1499788607000},"page":"580-586","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":23,"title":["Development of a Novel, Rapid Multiplex Polymerase Chain Reaction Assay for the Detection and Differentiation of\n Salmonella enterica<\/i>\n Serovars Enteritidis and Typhimurium Using Ultra-Fast Convection Polymerase Chain Reaction"],"prefix":"10.1177","volume":"14","author":[{"given":"Tae-Hoon","family":"Kim","sequence":"first","affiliation":[{"name":"Kyung Hee University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun Jin","family":"Hwang","sequence":"additional","affiliation":[{"name":"R&D Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Kyung Hee University"},{"name":"Kyung Hee University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,10,1]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.11.4984-4991.2001"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/520179"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2016.11.004"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2010.02.007"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2015.1950"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6968.12010"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2008.09.010"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2016.01.015"},{"key":"e_1_3_3_10_1","unstructured":"Hwang HJ. Three-stage thermal convection apparatus and uses thereof 2011; International Patent Application Publication No. WO 2011\/086497 A2. Publication date: July 21 2011."},{"key":"e_1_3_3_11_1","unstructured":"Hwang HJ Kim JH Jeong K. Method and apparatus for amplification of nucleic acid sequences by using thermal convection. 2009; US patent US 7 628 961 B2 December 8 2009."},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2016.11.007"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2014.07.004"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01233-09"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-69.7.1653"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.32.215"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2012.01.062"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-72.11.2350"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822013005000002"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01809.x"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfs.12019"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-009-0025-3"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.02.085"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0229"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2009.0430"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6968.12443"},{"key":"e_1_3_3_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2010.08.012"},{"key":"e_1_3_3_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2013.1701"}],"container-title":["Foodborne Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/fpd.2017.2290","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2017.2290","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2017.2290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:22:21Z","timestamp":1777443741000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/fpd.2017.2290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10]]},"references-count":27,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1089\/fpd.2017.2290"],"URL":"https:\/\/doi.org\/10.1089\/fpd.2017.2290","relation":{},"ISSN":["1535-3141","1556-7125"],"issn-type":[{"value":"1535-3141","type":"print"},{"value":"1556-7125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10]]}}}