{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T17:44:09Z","timestamp":1780595049282,"version":"3.54.1"},"reference-count":22,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:p>\n                    Approximately 20% of U.S. beef cattle receive prophylactic in-feed administration of chlortetracycline (CTC) to reduce bovine respiratory disease (BRD) incidence during the transition into feedlots. To determine the impact of prophylaxis on selected antimicrobial resistance genes (ARGs), 300 beef cattle were placed into 10 pens (30 head\/pen). Five \u201cCTC group\u201d pens received in-feed CTC (10\u2009mg\/lb of body weight\/d) from the fifth to ninth day after feedlot arrival, whereas the five \u201cControl group\u201d pens received no CTC. Fecal swabs and pen surface materials were collected for metagenomic DNA isolation on five sample occasions: arrival at the feedlot, 5 d posttreatment (dpt), and 27, 75, and 117 dpt. For each sample occasion, fecal samples and pen surface material samples were pooled by pen. Quantitative polymerase chain reaction was used to determine the abundances of 10 ARGs. Due to low detection percentages (%D) and quantification percentages (%Q), the abundances of five ARGs were not analyzed:\n                    <jats:italic toggle=\"yes\">aac(6<\/jats:italic>\n                    \u2032\n                    <jats:italic toggle=\"yes\">)-Ie-aph(2<\/jats:italic>\n                    \u2032\u2032\n                    <jats:italic toggle=\"yes\">)<\/jats:italic>\n                    (%D\u2009=\u200943%, %Q\u2009=\u20094%),\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CMY-2<\/jats:sub>\n                    (%D\u2009=\u200941%, %Q\u2009=\u20090%),\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    (%D\u2009=\u20090%, %Q\u2009=\u20090%),\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    (%D\u2009=\u200921%, %Q\u2009=\u200916%), and\n                    <jats:italic toggle=\"yes\">mecA<\/jats:italic>\n                    (%D\u2009=\u20094%, %Q\u2009=\u20090%). The %D and %Q for the ARGs\n                    <jats:italic toggle=\"yes\">aadA1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">erm<\/jats:italic>\n                    (B),\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (A)\n                    <jats:italic toggle=\"yes\">, tet<\/jats:italic>\n                    (B)\n                    <jats:italic toggle=\"yes\">,<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (M) were \u226598% and \u226590%, respectively. The abundances of\n                    <jats:italic toggle=\"yes\">aadA1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">erm<\/jats:italic>\n                    (B),\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (A)\n                    <jats:italic toggle=\"yes\">, tet<\/jats:italic>\n                    (B)\n                    <jats:italic toggle=\"yes\">,<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (M) resistance genes did not differ (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&gt;\u20090.05) between the CTC and control groups at any sampling time for feces or pen surface material. Although only 10 ARGs were examined in this study, the results suggest that a single 5-d in-feed CTC prophylaxis of beef cattle to prevent BRD has a negligible impact on the abundances of ARGs.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2018.2475","type":"journal-article","created":{"date-parts":[[2018,8,15]],"date-time":"2018-08-15T09:59:36Z","timestamp":1534327176000},"page":"689-697","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of In-Feed Chlortetracycline Prophylaxis in Beef Cattle on Antimicrobial Resistance Genes"],"prefix":"10.1177","volume":"15","author":[{"given":"Eric","family":"Miller","sequence":"first","affiliation":[{"name":"Clay Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Vikram","sequence":"additional","affiliation":[{"name":"Clay Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Getahun E.","family":"Agga","sequence":"additional","affiliation":[{"name":"Clay Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terrance M.","family":"Arthur","sequence":"additional","affiliation":[{"name":"Clay Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Clay Center"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,11,13]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01928-16"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1466252314000152"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/ame01753"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.15232\/pas.2015-01456"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.4.2503-2507.2004"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00055-09"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2014.05.019"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2009.10.007"},{"key":"e_1_3_4_10_1","unstructured":"FDA. Guidance for industry #213: New animal drugs and new animal drug combination products administered in or on medicated feed or drinking water of food producing animals: Recommendations for drug sponsors for voluntarily aligning product use conditions with GFI #209. 2013. Center for Veterinary Medicine. Food and Drug Admisitration. U.S. Department of Health and Human Services 2013. Available at: https:\/\/www.fda.gov\/downloads\/AnimalVeterinary\/GuidanceComplianceEnforcement\/GuidanceforIndustry\/UCM299624.pdf Accessed March22 2015."},{"key":"e_1_3_4_11_1","unstructured":"FDA. 2016 Summary report on antimicrobials sold or distributed for use in food-producing animals. Center for Veterinary Medicine. Food and Drug Admisitration. U.S. Department of Health and Human Services 2017. Available at: https:\/\/www.fda.gov\/downloads\/ForIndustry\/UserFees\/AnimalDrugUserFeeActADUFA\/UCM588085.pdf Accessed December8 2017."},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep05100"},{"key":"e_1_3_4_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080575"},{"key":"e_1_3_4_14_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.229.9.1389"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13023"},{"key":"e_1_3_4_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.02.034"},{"key":"e_1_3_4_18_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2007-0008"},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02227-15"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.15232\/S1080-7446(15)30452-6"},{"key":"e_1_3_4_21_1","unstructured":"USDA. Feedlot 2011 part IV: Health and health management on U.S. Feedlots with a capacity of 1 000 or more head. National Animal Health Monitoring System Animal and Plant Health Inspection Service U.S. Dept. of Agriculture. 2013. Available at: www.Aphis.Usda.Gov\/animal_health\/nahms\/feedlot\/downloads\/feedlot2011\/feed11_dr_partiv.Pdf Accessed April4 2015."},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01682-17"},{"key":"e_1_3_4_23_1","unstructured":"WHO. Critically important antimicrobials for human medicine\u20135th rev. World health organization. Geneva. 2017. Available at: www.Who.Int\/foodborne_disease\/resistance\/cia\/en\/ Accessed December5 2017."}],"container-title":["Foodborne Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/fpd.2018.2475","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2018.2475","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2018.2475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:22:41Z","timestamp":1777443761000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/fpd.2018.2475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11]]},"references-count":22,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1089\/fpd.2018.2475"],"URL":"https:\/\/doi.org\/10.1089\/fpd.2018.2475","relation":{},"ISSN":["1535-3141","1556-7125"],"issn-type":[{"value":"1535-3141","type":"print"},{"value":"1556-7125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11]]}}}