{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T20:01:45Z","timestamp":1784664105146,"version":"3.55.0"},"reference-count":46,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2019,3]]},"abstract":"\n Objectives:<\/jats:title>\n \n The occurrence of\n Listeria monocytogenes<\/jats:italic>\n was studied by using cultural and serological methods in cattle housed in a particular gaushala (cattle shelter) and organized dairy farm.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n A total of 1201 samples from cattle comprising blood (\n n<\/jats:italic>\n \u2009=\u2009207), milk (\n n<\/jats:italic>\n \u2009=\u2009203), vaginal swabs (\n n<\/jats:italic>\n \u2009=\u2009210), and serum (\n n<\/jats:italic>\n \u2009=\u2009207) from an organized farm (\n n<\/jats:italic>\n \u2009=\u2009210) and blood (\n n<\/jats:italic>\n \u2009=\u2009100), milk (\n n<\/jats:italic>\n \u2009=\u200974), vaginal swabs (\n n<\/jats:italic>\n \u2009=\u2009100), and serum (\n n<\/jats:italic>\n \u2009=\u2009100) from a gaushala (\n n<\/jats:italic>\n \u2009=\u2009100) were collected and analyzed for\n L. monocytogenes.<\/jats:italic>\n All samples excluding serum were analyzed for isolation and identification of\n L. monocytogenes<\/jats:italic>\n , while the serum samples were screened for seropositivity. The isolates were further subjected to assess their virulence potential (\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n ), biofilm formation ability, and antibiotic susceptibility patterns.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Four\n L. monocytogenes<\/jats:italic>\n strains were isolated from the cattle; three (0.48%) from the organized farm and one (0.36%) from the gaushala. On serological screening of cattle from the organized dairy farm, 16.42% were found to be positive for antibodies against listeriolysin O, while cattle from the gaushala revealed 36% seropositivity. Furthermore, on characterization of the isolates for their pathogenic potential and biofilm-forming ability, all were found to be pathogenic by both\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n assays and were weak to moderate biofilm formers. The minimum inhibition concentration (MIC) of recovered isolates revealed resistance for ampicillin by two\n L. monocytogenes<\/jats:italic>\n isolates (MIC >256\u2009\u03bcg\/mL), whereas three\n L. monocytogenes<\/jats:italic>\n isolates were intermediately resistant (MIC >4\u2009\u03bcg\/mL) and one resistant against amoxicillin (MIC >8\u2009\u03bcg\/mL). However, all four isolates were susceptible to gentamicin, cotrimoxazole, and erythromycin.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n Isolation of virulent and antibiotic-resistant strains of\n L. monocytogenes<\/jats:italic>\n warrants the need for epidemiological surveillance, antimicrobial susceptibility, and implementation of control measures to combat the occurrence of\n L. monocytogenes<\/jats:italic>\n infection in animals as well as humans.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1089\/fpd.2018.2494","type":"journal-article","created":{"date-parts":[[2019,1,11]],"date-time":"2019-01-11T11:04:33Z","timestamp":1547204673000},"page":"214-220","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Virulence Potential, Biofilm Formation, and Antibiotic Susceptibility of\n Listeria monocytogenes<\/i>\n Isolated from Cattle Housed in a Particular Gaushala (Cattle Shelter) and Organized Farm"],"prefix":"10.1177","volume":"16","author":[{"given":"Sunitha","family":"Ramanjeneya","sequence":"first","affiliation":[{"name":"Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudam Chandra","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richa","family":"Pathak","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jess","family":"Vergis","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satya Veer Singh","family":"Malik","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukhadeo B.","family":"Barbuddhe","sequence":"additional","affiliation":[{"name":"ICAR-National Research Centre on Meat, Hyderabad, India."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak B.","family":"Rawool","sequence":"additional","affiliation":[{"name":"Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,3,6]]},"reference":[{"key":"e_1_3_4_2_1","first-page":"16","article-title":"Listeriosis in large ruminants: A review","volume":"5","author":"Amene Y","year":"2016","unstructured":"Amene Y, , Firesbhat A. 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