{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:07:35Z","timestamp":1792001255340,"version":"4.4.0"},"reference-count":57,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T00:00:00Z","timestamp":1556668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Enterococcus faecalis<\/jats:italic>\n                    is not only a prevalent species among dairy microbial community but also a well-documented opportunistic pathogen. Food safety should exclude the possibility of consumer exposure to its virulence traits through consumption of dairy products. In this study, an integrated approach based on both phenotypic and genotypic methods was applied to investigate the incidence of antibiotic resistance and pathogenicity potential in 40\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    isolated from 10 Italian raw milk cheeses over a 13-year period (1997\u20132009). Among the 14 tested antibiotics, resistance to tetracycline, rifampicin, chloramphenicol, and erythromycin was observed, whereas vancomycin-resistant enterococci were not found. A high incidence (90% of strains) of the\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (M) gene emerged, whereas\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (K),\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (S),\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (L),\n                    <jats:italic toggle=\"yes\">int<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">erm<\/jats:italic>\n                    B genes were occasionally amplified (12.5%, 10%, 7.5%, 2.5% and 30%, respectively). No strain was positive for vancomycin-resistant determinants. Among the seven virulence determinants considered, the\n                    <jats:italic toggle=\"yes\">asa1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">gelE<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">esp<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">efaA<\/jats:italic>\n                    genes were harbored. No other gene encoding for either different virulence factors (\n                    <jats:italic toggle=\"yes\">cyl<\/jats:italic>\n                    A,\n                    <jats:italic toggle=\"yes\">hyl<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">ace<\/jats:italic>\n                    ) or amino acid decarboxylase activity (\n                    <jats:italic toggle=\"yes\">hdc<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">tdc<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">odc<\/jats:italic>\n                    ) was detected. Consequently,\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    isolated from raw milk cheeses does not represent a substantial reservoir of antimicrobial resistance and virulence factors if compared with clinical strains. However, this species occasionally harbors detrimental traits; thus, the possibility that it could be a route for transmission of pathogenic genes through dairy products should never be disregarded.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2018.2542","type":"journal-article","created":{"date-parts":[[2019,2,11]],"date-time":"2019-02-11T11:23:50Z","timestamp":1549884230000},"page":"359-367","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["Does\n                    <i>Enterococcus faecalis<\/i>\n                    from Traditional Raw Milk Cheeses Serve as a Reservoir of Antibiotic Resistance and Pathogenic Traits?"],"prefix":"10.1177","volume":"16","author":[{"given":"Tiziana","family":"Silvetti","sequence":"first","affiliation":[{"name":"Institute of Sciences of Food Production, Italian National Research Council, Milan, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Morandi","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, Italian National Research Council, Milan, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Brasca","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, Italian National Research Council, Milan, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,5,6]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.01.004"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2008.07.004"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00227"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-89132013000300014"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2009.03.005"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2009.10.002"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00922.x"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0213-005X(13)70126-5"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2004.12.021"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-10579"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2016.10.026"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-014-0742-1"},{"key":"e_1_3_2_14_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing: 27th ed. 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