{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T11:01:18Z","timestamp":1781780478527,"version":"3.54.5"},"reference-count":33,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>\n                    The aim of this study was to determine the effects of different temperatures and storage time on\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    growth,\n                    <jats:italic toggle=\"yes\">sea<\/jats:italic>\n                    gene expression, and synthesis of staphylococcal enterotoxin A (SEA) in the pasteurized and UHT-pasteurized milk. Pasteurized and UHT-pasteurized milk were inoculated with 3.98\u2009log\n                    <jats:sub>10<\/jats:sub>\n                    CFU\/mL of\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    (ATCC 13565). Inoculated milk samples were stored at 8\u00b0C, 15\u00b0C, and 22\u00b0C for 24, 48, and 72\u2009h, respectively. SEA synthesis was detected with a fully automated miniVIDAS instrument using the Enzyme-Linked Fluorescent Assay (ELFA) technology. The patterns of gene regulation were detected by quantitative reverse transcriptase PCR. The 2\n                    <jats:sup>\u2212\u0394\u0394CT<\/jats:sup>\n                    method has been used as a relative quantification strategy for gene expression responses data analysis. The results indicated that growth rate,\n                    <jats:italic toggle=\"yes\">sea<\/jats:italic>\n                    gene expression, and SEA synthesis were influenced by type of milk, storage time, and temperature. Incubation of milk at different temperatures (15\u00b0C and 22\u00b0C) and times was used to simulate inadequate transport and storage conditions. Storage of pasteurized milk at 22\u00b0C for 24\u2009h significantly upregulated the expression of\n                    <jats:italic toggle=\"yes\">sea<\/jats:italic>\n                    gene compared with milk stored at 8\u00b0C, which coincides with the achieved\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    number of 10\n                    <jats:sup>5<\/jats:sup>\n                    CFU\/mL and detected amount of SEA. In addition, storage of UHT-pasteurized milk at 22\u00b0C for 24\u2009h and at 15\u00b0C for 48\u2009h significantly upregulated the\n                    <jats:italic toggle=\"yes\">sea<\/jats:italic>\n                    gene expression compared with milk stored at 8\u00b0C, which coincides with the detected amount of SEA and the dynamics of\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    number change. It can, therefore, be concluded that implementing good hygiene practices to avoid pre- and post-heat treatment milk contamination and maintaining the cold chain at temperature &lt;8\u00b0C throughout the entire dairy production chain are of paramount importance to decrease the risk of staphylococcal food poisoning.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2018.2544","type":"journal-article","created":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T12:07:44Z","timestamp":1544530064000},"page":"282-289","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (\n                    <i>sea<\/i>\n                    ) Expression of\n                    <i>Staphylococcus aureus<\/i>\n                    in Milk"],"prefix":"10.1177","volume":"16","author":[{"given":"Milijana","family":"Babi\u0107","sequence":"first","affiliation":[{"name":"University of Belgrade","place":["Serbia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Paji\u0107","sequence":"additional","affiliation":[{"name":"University of Novi Sad","place":["Serbia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miodrag","family":"Radinovi\u0107","sequence":"additional","affiliation":[{"name":"University of Novi Sad","place":["Serbia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanko","family":"Bobo\u0161","sequence":"additional","affiliation":[{"name":"University of Novi Sad","place":["Serbia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sne\u017eana","family":"Bulaji\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade","place":["Serbia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Nikoli\u0107","sequence":"additional","affiliation":[{"name":"Institute of Meat Hygiene and Technology","place":["Serbia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branko","family":"Velebit","sequence":"additional","affiliation":[{"name":"Institute of Meat Hygiene and Technology","place":["Serbia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,4,10]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins2071751"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.15567\/mljekarstvo.2018.0102"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(94)90157-0"},{"key":"e_1_3_3_5_1","first-page":"418","article-title":"Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food\u201410 states, 2009","volume":"59","author":"Centers for Disease Control","year":"2010","unstructured":"Centers for Disease Control. Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food\u201410 states, 2009. MMWR Morb Mortal Wkly Rep, 2010; 59:418\u2013422.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2008.06.032"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-4565.2002.tb00340.x"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins9120407"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01736-09"},{"key":"e_1_3_3_10_1","first-page":"197","article-title":"The Euroean Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2016","volume":"15","author":"EFSA","year":"2017","unstructured":"EFSA. The Euroean Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2016. 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