{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:39:30Z","timestamp":1790501970748,"version":"4.1.0"},"reference-count":32,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T00:00:00Z","timestamp":1559260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2019,6]]},"abstract":"\n Campylobacter<\/jats:italic>\n is the leading bacterial cause of human enteritis in developed countries. Human campylobacteriosis is commonly associated with the consumption of undercooked, contaminated chicken, a natural host of\n Campylobacter<\/jats:italic>\n . Thus, the control of\n Campylobacter<\/jats:italic>\n colonization in poultry at the farm level would reduce the risk of human exposure to this pathogen. Vaccination is an attractive intervention measure to mitigate\n Campylobacter<\/jats:italic>\n in poultry. Our recent studies have demonstrated that the outer-membrane proteins CmeC (an essential component of CmeABC multidrug efflux pump) and CfrA (ferric enterobactin receptor) are feasible candidates for immune intervention against\n Campylobacter<\/jats:italic>\n . By targeting these two promising vaccine candidates, live attenuated\n Salmonella<\/jats:italic>\n -vectored vaccines were developed and evaluated in this study. Briefly, the\n cfrA<\/jats:italic>\n and\n cmeC<\/jats:italic>\n genes were cloned into expression vector pYA3493 and transferred into\n Salmonella enterica<\/jats:italic>\n serovar Typhimurium \u03c78914, the USDA licensed live attenuated vaccine strain. The oral live\n Salmonella<\/jats:italic>\n vaccines producing CfrA or CmeC (truncated or full length) were successfully constructed by using delicate molecular manipulation despite the challenge due to the potential toxic effect of the cloned gene product in the\n Escherichia coli<\/jats:italic>\n host. Expression and membrane localization of the target protein in the vaccines were confirmed by immunoblotting. The efficacies of the two live vaccines that produce full-length CfrA or CmeC were evaluated by using broiler chickens. However, oral vaccination of chickens failed to trigger significant systemic and intestinal mucosal immune responses and, consequently, did not confer protection against\n Campylobacter jejuni<\/jats:italic>\n colonization chickens. The vaccination regimens of the constructed live\n Salmonella<\/jats:italic>\n -vectored vaccine need to be optimized in future studies.\n <\/jats:p>","DOI":"10.1089\/fpd.2018.2561","type":"journal-article","created":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T11:41:52Z","timestamp":1552477312000},"page":"399-410","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Development and Evaluation of Two Live\n Salmonella<\/i>\n -Vectored Vaccines for\n Campylobacter<\/i>\n Control in Broiler Chickens"],"prefix":"10.1177","volume":"16","author":[{"given":"Lindsay Jones","family":"Adams","sequence":"first","affiliation":[{"name":"Department of Animal Science, The University of Tennessee, Knoxville, Tennessee."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ximin","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Animal Science, The University of Tennessee, Knoxville, Tennessee."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Animal Science, The University of Tennessee, Knoxville, Tennessee."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,5,31]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/319760"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1637\/9004-072709-Reg.1"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(96)05683-8"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/08820138909112265"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.12.002"},{"key":"e_1_3_3_7_1","first-page":"15282","volume-title":"USDA, ed. FSIS Notice","author":"Food Safety and Inspection Service","year":"2011","unstructured":"Food Safety and Inspection Service. New performance standards for Salmonella and Campylobacter in chilled carcasses at young chicken and turkey establishments. USDA, ed. FSIS Notice, 2011, pp. 15282\u201315290."},{"key":"e_1_3_3_8_1","unstructured":"Friedman CR Neimann J Wegener HC Tauxe RV . Epidemiology of Campylobacter jejuni infections in the United States and other industrialized nations. In: Nachamkin I andBlaser MJ eds. Campylobacter. Washington D.C.: ASM Press 2000 pp. 121\u2013138."},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(90)90464-3"},{"key":"e_1_3_3_10_1","first-page":"50684","article-title":"Rapid protocol for preparation of electrocompetent Escherichia coli and Vibrio cholerae","author":"Gonzales MF","year":"2013","unstructured":"Gonzales MF, , Brooks T, , Pukatzki SU, , Provenzano D. Rapid protocol for preparation of electrocompetent Escherichia coli and Vibrio cholerae. 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[Immunogenicity of attenuated Salmonella choleraesuis vaccine strain expressing immunogenic genes of Mycoplasma hyopneumoniae in mice]. Wei Sheng Wu Xue Bao, 2011; 51:1270\u20131277.","journal-title":"Wei Sheng Wu Xue Bao"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2006.3.393"},{"key":"e_1_3_3_23_1","first-page":"693","article-title":"Construction of an Asd+ expression-cloning vector\u2014Stable maintenance and high-level expression of cloned genes in a Salmonella vaccine strain","volume":"6","author":"Nakayama K","year":"1988","unstructured":"Nakayama K, , Kelly SM, , Curtiss R. Construction of an Asd+ expression-cloning vector\u2014Stable maintenance and high-level expression of cloned genes in a Salmonella vaccine strain. 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