{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T13:17:00Z","timestamp":1781011020128,"version":"3.54.1"},"reference-count":45,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2020,5,1]],"date-time":"2020-05-01T00:00:00Z","timestamp":1588291200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2020,5]]},"abstract":"\n Toxoplasma gondii<\/jats:italic>\n and\n Neospora caninum<\/jats:italic>\n infections can cause reproductive failure in animals, including goats, and toxoplasmosis is one of the most important foodborne diseases. However, information on the molecular prevalence and genetic characterization of\n T. gondii<\/jats:italic>\n and\n N. caninum<\/jats:italic>\n in the tissues of goats in China is limited. In this study, brain samples of 422 slaughtered goats were collected from slaughterhouses in Henan and Anhui provinces, Central China, and examined for the presence of\n T. gondii<\/jats:italic>\n and\n N. caninum<\/jats:italic>\n by nested polymerase chain reaction (PCR) based on\n B1<\/jats:italic>\n and\n NC5<\/jats:italic>\n genes, respectively. The prevalence of\n T. gondii<\/jats:italic>\n and\n N. caninum<\/jats:italic>\n DNA was 5.2% and 2.8%, respectively. No significant differences were found between the prevalences of two parasite infections and animal age, sex, and region (\n p<\/jats:italic>\n \u2009>\u20090.05). Two of 22\n T. gondii<\/jats:italic>\n -positive samples were completely genotyped at 11 genetic markers (\n SAG1<\/jats:italic>\n , [\n 3\u2032<\/jats:italic>\n +\n 5\u2032<\/jats:italic>\n ]\n SAG2<\/jats:italic>\n , alternative\n SAG2<\/jats:italic>\n [\n alt. SAG2<\/jats:italic>\n ],\n SAG3<\/jats:italic>\n ,\n BTUB<\/jats:italic>\n ,\n GRA6<\/jats:italic>\n ,\n c22-8<\/jats:italic>\n ,\n c29-2<\/jats:italic>\n ,\n L358<\/jats:italic>\n ,\n PK1<\/jats:italic>\n , and\n Apico<\/jats:italic>\n ) using PCR\u2013restriction fragment length polymorphism, and were identified as genotype ToxoDB no. 225, which has not been previously reported in goats in any country worldwide. For\n N. caninum<\/jats:italic>\n , two different sequences at the\n ITS1<\/jats:italic>\n region, three genotypes at the\n MS5<\/jats:italic>\n microsatellite locus, and one genotype at the\n MS8<\/jats:italic>\n locus were identified. This study showed that\n T. gondii<\/jats:italic>\n and\n N. caninum<\/jats:italic>\n are moderately prevalent in goats in Central China; however, it should be emphasized that\n T. gondii<\/jats:italic>\n prevalence in goats poses a potential health threat for consumers in the investigated areas. To the best of our knowledge, this is the first report on the genetic characterization of\n N. caninum<\/jats:italic>\n isolates from goats in China. Our results have important implications for a better understanding of the genetic diversity of these parasites in China.\n <\/jats:p>","DOI":"10.1089\/fpd.2019.2726","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T14:46:00Z","timestamp":1584369960000},"page":"348-356","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Molecular Detection and Genotyping of\n Toxoplasma gondii<\/i>\n and\n Neospora caninum<\/i>\n in Slaughtered Goats in Central China"],"prefix":"10.1177","volume":"17","author":[{"given":"Weifeng","family":"Qian","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenchao","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaochao","family":"Lv","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongzhen","family":"Bai","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianqi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2020,5,7]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-018-1029-4"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1515\/ap-2018-0083"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2013.01.006"},{"key":"e_1_3_4_5_1","first-page":"236","article-title":"Experimental fetal and transplacental Neospora infection in the nonhuman primate","volume":"71","author":"Barr BC","year":"1994","unstructured":"Barr BC, , Conrad PA, , Sverlow KW, , Tarantal AF, , Hendrickx AG. 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