{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:45:51Z","timestamp":1787161551184,"version":"build-2736575974"},"reference-count":33,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:p>\n                    Retail meats are one of the main routes for spreading antimicrobial-resistant bacteria (ARB) from livestock to humans through the food chain. In African countries, retail meats are often sold at roadside butcheries without chilling or refrigeration. Retail meats in those butcheries are suspected to be contaminated by ARB, but it was not clear. In this study, we tested for the presence of antimicrobial-resistant\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    from retail meats (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200964) from roadside butcheries in Kampala, Uganda. The meat surfaces were swabbed and inoculated on PetriFilm SEC agar to isolate\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    . We successfully isolated\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    from 90.6% of these retail meat samples. We identified the phylogenetic type, antimicrobial susceptibility, and antimicrobial resistance genes prevalence between retail meat isolates (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200989). Phylogenetic type B1 was identified from 70.8% of the retail meat isolates, suggesting that the isolates originated primarily from fecal contamination during meat processing. Tetracycline (TET)-resistant isolates with\n                    <jats:italic toggle=\"yes\">tetA<\/jats:italic>\n                    and\/or\n                    <jats:italic toggle=\"yes\">tetB<\/jats:italic>\n                    gene(s) were the most frequently detected (28.1%), followed by ampicillin (AMP) resistance genes with\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    (15.7%,) and sulfamethoxazole\u2013trimethoprim (SXT) resistance genes with\n                    <jats:italic toggle=\"yes\">sul2<\/jats:italic>\n                    (15.7%). No extended-spectrum beta-lactamase-producing isolates were detected. A conjugation assay showed that resistance to AMP, TET, and SXT could be simultaneously transferred to recipients. These findings suggest that antimicrobial-resistant\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    can easily be transferred from farms to tables from retail meats obtained from roadside butcheries.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2020.2796","type":"journal-article","created":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T11:16:11Z","timestamp":1592478971000},"page":"666-671","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Isolation and Characterization of Antimicrobial-Resistant\n                    <i>Escherichia coli<\/i>\n                    from Retail Meats from Roadside Butcheries in Uganda"],"prefix":"10.1177","volume":"17","author":[{"given":"Torahiko","family":"Okubo","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University Graduate School of Health Sciences, Sapporo, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montira","family":"Yossapol","sequence":"additional","affiliation":[{"name":"Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan."},{"name":"Office of Academic Affairs, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiori","family":"Ikushima","sequence":"additional","affiliation":[{"name":"Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Kakooza","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacy, Clinics and Comparative Medicine, School of Veterinary Medicine and Animal Resources, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eddie M.","family":"Wampande","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacy, Clinics and Comparative Medicine, School of Veterinary Medicine and Animal Resources, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda."},{"name":"Central Diagnostic Laboratory, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Asai","sequence":"additional","affiliation":[{"name":"Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan."},{"name":"Education and Research Center for Food Animal Health, Gifu University (GeFAH), Gifu, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayaka","family":"Tsuchida","sequence":"additional","affiliation":[{"name":"Chubu University Academy of Emerging Sciences, Kasugai, Japan."},{"name":"Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Ohya","sequence":"additional","affiliation":[{"name":"Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan."},{"name":"Education and Research Center for Food Animal Health, Gifu University (GeFAH), Gifu, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumito","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Microbial Genomics and Ecology, Office of Academic Research and Industry-Government Collaboration, Academy of Hiroshima University, Hiroshima, Japan."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Kabasa","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Clinical and Comparative Medicine, School of Veterinary Medicine and Animal Resources, Makerere University, Kampala, Uganda."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunari","family":"Ushida","sequence":"additional","affiliation":[{"name":"Chubu University Academy of Emerging Sciences, Kasugai, Japan."},{"name":"Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, 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