{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:28:12Z","timestamp":1791646092659,"version":"4.3.4"},"reference-count":66,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Listeria monocytogenes<\/jats:italic>\n                    is a foodborne pathogen of global relevance that causes outbreaks and sporadic cases of listeriosis, acquired through the consumption of contaminated products, including milk or meat products and ready-to-eat meat products subjected to intensive handling. The objective of the present study was to classify\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    isolated from various food-related sources in the Federal District of Brazil and surrounding areas to sequence internalin A (\n                    <jats:italic toggle=\"yes\">inlA<\/jats:italic>\n                    ) genes from these isolates and assess their adhesion and invasion capacity using Caco-2 cells. In addition, 15 were classified as group I, 3 as group II, and 7 classified as group IV. Premature stop codons (PMSCs) at the nucleotide position 976 (GAA\u2192TAA) of the\n                    <jats:italic toggle=\"yes\">inlA<\/jats:italic>\n                    gene were identified in 5 of the 25 isolates. Adhesion and invasion tests in Caco-2 cells showed that all the isolates were capable of adhesion and cellular invasion, with isolates containing PMSCs exhibiting on average higher invasion capacity than those without PMSCs (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.041) and a median of adhesion very distinctive from those without stop codons. These results are the first report of PMSCs in the\n                    <jats:italic toggle=\"yes\">inlA<\/jats:italic>\n                    gene of\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    from the Federal District of Brazil and Brazil.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2020.2855","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T12:02:26Z","timestamp":1608292946000},"page":"243-252","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Assessment of Internalin A Gene Sequences and Cell Adhesion and Invasion Capacity of\n                    <i>Listeria monocytogenes<\/i>\n                    Strains Isolated from Foods of Animal and Related Origins"],"prefix":"10.1177","volume":"18","author":[{"given":"Margareti","family":"Medeiros","sequence":"first","affiliation":[{"name":"Food Molecular Microbiology Laboratory, ASS 128\/10, Faculty of Agronomy and Veterinary Medicine, University of Bras\u00edlia (UnB), Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virgilio Hipolito Lemos de","family":"Castro","sequence":"additional","affiliation":[{"name":"Food Molecular Microbiology Laboratory, ASS 128\/10, Faculty of Agronomy and Veterinary Medicine, University of Bras\u00edlia (UnB), Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Lourdes Arrais de Alencar","family":"Mota","sequence":"additional","affiliation":[{"name":"Food Molecular Microbiology Laboratory, ASS 128\/10, Faculty of Agronomy and Veterinary Medicine, University of Bras\u00edlia (UnB), Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marita Gimenez","family":"Pereira","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine Cristina Pereira","family":"De Martinis","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Perecmanis","sequence":"additional","affiliation":[{"name":"Food Molecular Microbiology Laboratory, ASS 128\/10, Faculty of Agronomy and Veterinary Medicine, University of Bras\u00edlia (UnB), Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela Patricia","family":"Santana","sequence":"additional","affiliation":[{"name":"Food Molecular Microbiology Laboratory, ASS 128\/10, Faculty of Agronomy and Veterinary Medicine, University of Bras\u00edlia (UnB), Bras\u00edlia, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,4,7]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjm.2017.01.010"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1078\/0723202041438518"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/viru.2.5.17703"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01874-13"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11483-009-9128-5"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01564-10"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evv131"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00146"},{"key":"e_1_3_4_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03305-13"},{"key":"e_1_3_4_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2016.10.033"},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins12010060"},{"key":"e_1_3_4_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02857-19"},{"key":"e_1_3_4_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3819-3822.2004"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.12.007"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.03.004"},{"key":"e_1_3_4_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00030-15"},{"key":"e_1_3_4_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02912-06"},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02553-10"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01285-06"},{"key":"e_1_3_4_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.11.2822-2829.1987"},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0101-20612010000400006"},{"key":"e_1_3_4_23_1","first-page":"293","article-title":"A novel mutation leading to a premature stop codon in inlA of Listeria monocytogenes isolated from neonatal listeriosis","volume":"38","author":"Gelb\u00ed\u010dov\u00e1 T","year":"2015","unstructured":"Gelb\u00ed\u010dov\u00e1 T, , Kol\u00e1\u010dkov\u00e1 I, , Pant\u016f\u010dek R, , Karp\u00ed\u0161kov\u00e1 R. A novel mutation leading to a premature stop codon in inlA of Listeria monocytogenes isolated from neonatal listeriosis. New Microbiol, 2015; 38:293\u2013296.","journal-title":"New Microbiol"},{"key":"e_1_3_4_24_1","first-page":"251","volume-title":"Listeria monocytogenes","author":"Graves LM","year":"1999","unstructured":"Graves LM, , Swaminathan B, , Hunter SB. 1999. Subtyping Listeria monocytogenes. In Ryser ET, Marth EH, eds. Listeria, Listeriosis and Food Safety. New York: Marcel Dekker, Inc. 1999, pp. 251\u2013297."},{"key":"e_1_3_4_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.10.5833-5841.2004"},{"key":"e_1_3_4_26_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822006000100006"},{"key":"e_1_3_4_27_1","doi-asserted-by":"publisher","DOI":"10.1086\/420853"},{"key":"e_1_3_4_28_1","unstructured":"Jawetz E Melnick JL Adelberg EA. Medical Microbiology 20th ed. Rio de Janeiro: Guanabara Koogan 1998."},{"key":"e_1_3_4_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2009.11.008"},{"key":"e_1_3_4_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03341-12"},{"key":"e_1_3_4_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2012.06.047"},{"key":"e_1_3_4_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-14-15"},{"key":"e_1_3_4_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2013.06.008"},{"key":"e_1_3_4_34_1","first-page":"287","article-title":"Pheno and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the state of S\u00e3o Paulo, Brazil. Braz","volume":"38","author":"Lemes-Marques EG","year":"2007","unstructured":"Lemes-Marques EG, , Cruz CD, , Destro MT. Pheno and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the state of S\u00e3o Paulo, Brazil. Braz. J Microbiol, 2007; 38:287\u2013292.","journal-title":"J Microbiol"},{"key":"e_1_3_4_35_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-69.11.2640"},{"key":"e_1_3_4_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.08.007"},{"key":"e_1_3_4_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00928-06"},{"key":"e_1_3_4_38_1","first-page":"626","article-title":"Listeria monocytogenes: Monitoring of this biological danger in the chicken production chain in southern Rio Grande do Sul","volume":"29","author":"Nal\u00e9rio ES","year":"2009","unstructured":"Nal\u00e9rio ES, , de Ara\u00fajo MR, , Mendon\u00e7a KS, , Bassani MT, , da Silva WP. Listeria monocytogenes: Monitoring of this biological danger in the chicken production chain in southern Rio Grande do Sul. Food Sci Technol, 2009; 29:626\u2013630.","journal-title":"Food Sci Technol"},{"key":"e_1_3_4_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8764-8772.2005"},{"key":"e_1_3_4_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.2.646-653.2001"},{"key":"e_1_3_4_41_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/007310-0"},{"key":"e_1_3_4_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-7552-2"},{"key":"e_1_3_4_43_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e16-12-0851"},{"key":"e_1_3_4_44_1","doi-asserted-by":"publisher","DOI":"10.1590\/s0100-736x2016001000007"},{"key":"e_1_3_4_45_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822001000200008"},{"key":"e_1_3_4_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000900"},{"key":"e_1_3_4_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3608"},{"key":"e_1_3_4_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00555-18"},{"key":"e_1_3_4_49_1","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-142-1-173"},{"key":"e_1_3_4_50_1","first-page":"3868","article-title":"Culturing and applications of rotating wall vessel bioreactor derived 3D epithelial cell models","volume":"62","author":"Radtke AL","year":"2012","unstructured":"Radtke AL, , Herbst-Kralovetz MM. Culturing and applications of rotating wall vessel bioreactor derived 3D epithelial cell models. J Vis Exp, 2012; 62:3868.","journal-title":"J Vis Exp"},{"key":"e_1_3_4_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000146"},{"key":"e_1_3_4_52_1","first-page":"1","volume-title":"Listeria","author":"Rocourt J","year":"2007","unstructured":"Rocourt J, , Buchrieser C. 2007. The genus Listeria and Listeria monocytogenes: Phylogenetic position, taxonomy, and identification. In Ryser ET, Marth EH, eds. Listeria, Listeriosis and Food Safety. Boca Raton, FL: CRC Press, 2007, pp. 1\u201312."},{"key":"e_1_3_4_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200300624"},{"key":"e_1_3_4_54_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1676-24442003000200004"},{"key":"e_1_3_4_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.004"},{"key":"e_1_3_4_56_1","unstructured":"StataCorp. Stata: Release 12. Statistical Software. College Station TX: StataCorp LP. 2011."},{"key":"e_1_3_4_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13099-019-0307-8"},{"key":"e_1_3_4_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2006.01.004"},{"key":"e_1_3_4_59_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/540204"},{"key":"e_1_3_4_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01138-08"},{"key":"e_1_3_4_61_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-16-145"},{"key":"e_1_3_4_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02651-09"},{"key":"e_1_3_4_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01008-10"},{"key":"e_1_3_4_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-013-9135-1"},{"key":"e_1_3_4_65_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-838220131071"},{"key":"e_1_3_4_66_1","article-title":"Listeria monocytogenes prevalence and characteristics in retail raw foods in China","volume":"10","author":"Wu S","year":"2015","unstructured":"Wu S, , Wu Q, , Zhang J, , ChenM, Yan Z, , Hu H. Listeria monocytogenes prevalence and characteristics in retail raw foods in China. PLoS One, 2015; 10:e0136682.","journal-title":"PLoS One"},{"key":"e_1_3_4_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.04.059"}],"container-title":["Foodborne Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/fpd.2020.2855","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2020.2855","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2020.2855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:23:49Z","timestamp":1777443829000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/fpd.2020.2855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":66,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,4]]}},"alternative-id":["10.1089\/fpd.2020.2855"],"URL":"https:\/\/doi.org\/10.1089\/fpd.2020.2855","relation":{},"ISSN":["1535-3141","1556-7125"],"issn-type":[{"value":"1535-3141","type":"print"},{"value":"1556-7125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4]]}}}