{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T18:39:38Z","timestamp":1788374378571,"version":"build-2803163510"},"reference-count":41,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:p>\n                    The prevalence of\n                    <jats:italic toggle=\"yes\">Listeria monocytogenes<\/jats:italic>\n                    in raw beef and in slaughterhouse environments was investigated from April 2019 to February 2020. Three hundred raw beef samples were purchased from 50 retailers and 10 restaurants (5 samples per source). One hundred and thirty-four samples from slaughterhouse environments were collected by swabbing (10\u2009\u00d7\u200910\u2009cm) the surfaces, gloves, splitting saw, and drains.\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    was detected and identified according to the method described in ISO 11290-1, and confirmed by 16S rRNA sequencing.\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    was detected in raw beef (2\/300, 0.7%), gloves used in carcass splitting (6\/21, 28.6%), the splitting saw (1\/18, 5.6%), and the drain zone (1\/15, 6.7%). All isolates were serotype 1\/2a or 1\/2c, based on screening using multiplex PCR-based serogrouping assay and serotyping kit for O-H antigens. Pulsed-field gel electrophoresis (PFGE) following\n                    <jats:italic toggle=\"yes\">Apa<\/jats:italic>\n                    I digestion of eight PFGE pulsotypes and four PFGE groups were identified. Biofilm formation analysis using Crystal Violet staining revealed the highest biofilm formation in strain LM-16, followed by D190613. Although\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    isolates were susceptible to most antimicrobials, some resistance to penicillin (8\/15, 53.3%) and tetracycline (2\/15, 13.3%) was observed. Through PFGE, G190426, G190829, and G200210 isolated from the same location in this study were genetically homologous similar to the LM-16 strain, previously isolated from beef carcass in 2006. These results suggest that LM-16 has been continuously present in biofilms in the slaughterhouse environments since 2006. Our study indicates that\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    contamination in raw beef could consistently occur during beef processing in slaughterhouse environments through contact with gloves, splitting saws, and drains.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2020.2903","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T12:28:38Z","timestamp":1619440118000},"page":"419-425","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":20,"title":["Prevalence, Characterization, and Antimicrobial Susceptibility of\n                    <i>Listeria monocytogenes<\/i>\n                    from Raw Beef and Slaughterhouse Environments in Korea"],"prefix":"10.1177","volume":"18","author":[{"given":"Yong-Seok","family":"Jang","sequence":"first","affiliation":[{"name":"Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul, South Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-San","family":"Moon","sequence":"additional","affiliation":[{"name":"Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, South Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Jeong","family":"Kang","sequence":"additional","affiliation":[{"name":"Bacterial Disease Division, Animal and Plant Quarantine Agency, Gimcheon, South Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongryeoul","family":"Bae","sequence":"additional","affiliation":[{"name":"Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul, South Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5720-0538","authenticated-orcid":false,"given":"Kun-Ho","family":"Seo","sequence":"additional","affiliation":[{"name":"Center for One Health, College of Veterinary Medicine, Konkuk University, Seoul, South Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2021,6,3]]},"reference":[{"key":"e_1_3_6_2_1","unstructured":"[APQA] Animal and Plant Quarantine Agency. 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Performance Standards for Antimicrobial Susceptibility Testing; Fifteenth Informational Supplement. CLSI Document M100-25. Wayne PA: CLSI 2015."},{"key":"e_1_3_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2007.03688.x"},{"key":"e_1_3_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3819-3822.2004"},{"key":"e_1_3_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01285-06"},{"key":"e_1_3_6_15_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-53.9.739"},{"key":"e_1_3_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(02)00471-6"},{"key":"e_1_3_6_17_1","unstructured":"International Organization for Standardization. Microbiology of Food and Animal Feeding Stuffs\u2014Horizontal Method for the Detection and Enumeration of Listeria monocytogenes\u2014Part 1: Detection Method. Amendment 1: Modification of the Isolation Media and the Haemolysis Test and Inclusion of Precision Data. ISO 11290-1: 1996\/Amd 1 2004a. 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