{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:54:03Z","timestamp":1788274443533,"version":"build-2803163510"},"reference-count":35,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Listeria monocytogenes<\/jats:italic>\n                    represents a high risk in food and can trigger potentially fatal listeriosis. The objective of this study was to detect\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    in food using the LAMP method in a fast, specific, sensitive manner and thus to preventively test food for the presence of the target species. The reaction was performed and established using the portable real-time fluorometer Genie\n                    <jats:sup>\u00ae<\/jats:sup>\n                    II (OptiGene Ltd., Horsham, United Kingdom). In this new assay, six LAMP primers targeted the\n                    <jats:italic toggle=\"yes\">mpl<\/jats:italic>\n                    -gene sequence of\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    . A total of 148 different isolates, including 105\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    and 43 non-\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    strains, were tested. Analytical sensitivity was determined based on different DNA- and cell concentrations. The detection limit with a detection rate of 100% was 5\u2009pg of DNA or 275 colony-forming units (CFU) per reaction. Artificially contaminated minced beef and grated mozzarella were also tested. The assay was 100% successful to detect an initial bacterial contamination of 0.4\u20134 CFU g\n                    <jats:sup>\u22121<\/jats:sup>\n                    food after 24\u2009h enrichment in half-Fraser broth. Finally, natively contaminated samples were tested in comparison to the microbiological reference method and real-time polymerase chain reaction. Native sample testing revealed 100% consistent findings between LAMP and the standard culture method after first enrichment for 24\u2009h. In addition, a rapid colony-confirmation method was established that enabled reliable identification of\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    isolates on different selective culture media using a simplified DNA extraction by boiling. This study showed that the developed assay was able to determine whether a food is safe with respect to the food-safety criteria of 100 CFU per gram, according to standards of the European Union, for\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    and provided faster results than the cultural reference method.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2021.0080","type":"journal-article","created":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T10:31:12Z","timestamp":1643625072000},"page":"463-472","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["<i>Mpl<\/i>\n                    -Gene-Based Loop-Mediated Isothermal Amplification Assay for Specific and Rapid Detection of\n                    <i>Listeria monocytogenes<\/i>\n                    in Various Food Samples"],"prefix":"10.1177","volume":"19","author":[{"given":"Annemarie","family":"Busch","sequence":"first","affiliation":[{"name":"Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Schotte","sequence":"additional","affiliation":[{"name":"Department A (Veterinary Medicine), Central Institute of the Bundeswehr Medical Service Kiel, Kronshagen, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine","family":"Pl\u00f6tz","sequence":"additional","affiliation":[{"name":"Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5189-8381","authenticated-orcid":false,"given":"Amir","family":"Abdulmawjood","sequence":"additional","affiliation":[{"name":"Institute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Hannover, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,7,11]]},"reference":[{"key":"e_1_3_5_2_1","first-page":"87","article-title":"Rapid detection of Listeria monocytogenes strains isolated from clinical and non-clinical samples by loop-mediated isothermal amplification method (LAMP)","volume":"4","author":"Aleyasin N","year":"2018","unstructured":"Aleyasin N, , Karimaei S, , Talebi M, , Kashanian M, , Irajian GR. Rapid detection of Listeria monocytogenes strains isolated from clinical and non-clinical samples by loop-mediated isothermal amplification method (LAMP). Infect Epidemiol Microbiol, 2018; 4:87\u201392.","journal-title":"Infect Epidemiol Microbiol"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00130-16"},{"key":"e_1_3_5_4_1","unstructured":"Anonymous. DIN EN ISO 6887-5:2011-01; Microbiology of food and animal feeding stuffs\u2014Preparation of test samples initial suspension and decimal dilutions for microbiological examination\u2014Part 5: Specific rules for the preparation of milk and milk products (ISO 6887-5:2010); German version EN ISO 6887-5:2010. Beuth Publishing DIN 2011."},{"key":"e_1_3_5_5_1","unstructured":"Anonymous. DIN EN ISO 6887-1:2017-07; Microbiology of the food chain\u2014Preparation of test samples initial suspension and decimal dilutions for microbiological examination\u2014Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017); German version EN ISO 6887-1:2017. Beuth Publishing DIN 2017a."},{"key":"e_1_3_5_6_1","volume-title":"Listeria monocytogenes","author":"Anonymous","year":"2017","unstructured":"Anonymous. DIN EN ISO 11290-1:2017-09; Microbiology of the food chain\u2014Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp.\u2014Part 1: Detection method (ISO 11290-1:2017); German version EN ISO 11290-1:2017. Beuth Publishing DIN 2017b."},{"key":"e_1_3_5_7_1","volume-title":"Listeria monocytogenes","author":"Anonymous","year":"2017","unstructured":"Anonymous. DIN EN ISO 11290-2:2017-09; Microbiology of the food chain\u2014Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp.\u2014Part 2: Enumeration method (ISO 11290-2:2017); German version EN ISO 11290-2:2017. Beuth Publishing DIN 2017c."},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(96)01161-0"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.18-0329"},{"key":"e_1_3_5_10_1","unstructured":"BfR. Listeriosis: Rare but dangerous for the elderly expectant mothers and immunocomprimised persons. German Federal Institute for Risk-Assessment (BfR)\u2014Press release 2018; accessed November3 2020."},{"key":"e_1_3_5_11_1","first-page":"5383","article-title":"Evaluation of two loop-mediated isothermal amplification methods for the detection of Salmonella Enteritidis and Listeria monocytogenes in artificially contaminated ready-to-eat fresh produce.\u201d","volume":"4","author":"Birmpa A","year":"2015","unstructured":"Birmpa A, , Kalogeropoulos K, , Kokkinos P, , Vantarakis A. Evaluation of two loop-mediated isothermal amplification methods for the detection of Salmonella Enteritidis and Listeria monocytogenes in artificially contaminated ready-to-eat fresh produce.\u201d. Ital J Food Saf, 2015; 4:5383.","journal-title":"Ital J Food Saf"},{"key":"e_1_3_5_12_1","volume-title":"Listeria monocytogenes","author":"BVL","year":"2013","unstructured":"BVL. Official collection of testing methods according to \u00a7 64 LFGB; BVL method L 03.00\u201340, testing of foodstuffs, detection of Listeria monocytogenes in cheese, real-time PCR method. Official collection of examination procedures according to \u00a7 64 LFGB 2013; Federal Office of Consumer Protection and Food Safety (BVL)."},{"key":"e_1_3_5_13_1","article-title":"The public health risk posed by Listeria monocytogenes in frozen fruit and vegetables including herbs, blanched during processing","volume":"18","author":"EFSA; Hazards EP","year":"2020","unstructured":"EFSA; Hazards EP, , Koutsoumanis K, , Alvarez-Ord\u00f3\u00f1ez A, et al., European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA). The public health risk posed by Listeria monocytogenes in frozen fruit and vegetables including herbs, blanched during processing. EFSA J Sci Opin, 2020a;18:e06092.","journal-title":"EFSA J Sci Opin"},{"key":"e_1_3_5_14_1","unstructured":"EFSA. Scientific Topic: Listeria. EFSA J Sci Top 2020b. https:\/\/www.efsa.europa.eu\/de\/topics\/topic\/listeria accessed November3 2020."},{"key":"e_1_3_5_15_1","unstructured":"EFSA; European Food Safety Authority and European Centre for Disease Prevention and Control (EFSA). The European Union One Health 2019 Zoonoses Report. EFSA J Sci Rep 2021. https:\/\/www.efsa.europa.eu\/de\/topics\/topic\/listeria accessed November3 2020."},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-55.8.639"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4359-4365.2003"},{"key":"e_1_3_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2005.04.016"},{"key":"e_1_3_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0740-0020(88)90004-4"},{"key":"e_1_3_5_20_1","doi-asserted-by":"crossref","unstructured":"Johnson AD. An extended IUPAC nomenclature code for polymorphic nucleic acids. 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