{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T06:18:51Z","timestamp":1791958731248,"version":"4.3.4"},"reference-count":61,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:p>\n                    Antimicrobials have been widely used in dairy farms to prevent and control dairy cattle diseases since 1960s. This led to the emergence of antimicrobial resistant bacteria (ARB) that, along with their antimicrobial resistance genes (ARGs), can spread from dairy farms to humans. Therefore, regular antimicrobial resistance (AMR) monitoring is important to implement proper mitigation measures. The objective of this study was to determine the prevalence of AMR and extended-spectrum beta-lactamases (ESBLs)-producing\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    in dairy cattle. A cross-sectional study was conducted in four dairy cattle farms (A\u2013D) in East Tennessee. A total of 80 samples consisting of 20 samples each of bulk tank milk, feces, dairy cattle manure-amended soil, and prairie soil adjacent to the farms were collected and cultured for the isolation of\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    . Tetracycline (TET\n                    <jats:sup>r<\/jats:sup>\n                    )-, third-generation cephalosporin (TGC\n                    <jats:sup>r<\/jats:sup>\n                    )- and nalidixic acid (NAL\n                    <jats:sup>r<\/jats:sup>\n                    )-resistant\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200988) were isolated and identified on agar media supplemented with TET, cefotaxime, and NAL, respectively. TGC\n                    <jats:sup>r<\/jats:sup>\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    were tested for ESBLs and other coselected ARGs. TET\n                    <jats:sup>r<\/jats:sup>\n                    (74%,\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200988) was the most common, followed by TGC\n                    <jats:sup>r<\/jats:sup>\n                    (20%) and NAL\n                    <jats:sup>r<\/jats:sup>\n                    (8%). Farms had significant (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.001) differences: the highest prevalence of TGC\n                    <jats:sup>r<\/jats:sup>\n                    (55%) and TET\n                    <jats:sup>r<\/jats:sup>\n                    (100%) were observed in farm D, while all NAL\n                    <jats:sup>r<\/jats:sup>\n                    isolates were from farm C. Over 83% of TGC\n                    <jats:sup>r<\/jats:sup>\n                    isolates (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200918) harbored ESBL gene\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    . Majority (78%) of the\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates were multidrug-resistant (MDR), being positive for beta-lactams (\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    ), TETs\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">A<\/jats:italic>\n                    ),\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">B<\/jats:italic>\n                    ),\n                    <jats:italic toggle=\"yes\">tet<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">M<\/jats:italic>\n                    )), sulfonamides (\n                    <jats:italic toggle=\"yes\">sul<\/jats:italic>\n                    2), aminoglycosides (\n                    <jats:italic toggle=\"yes\">strA<\/jats:italic>\n                    ), and phenicols (\n                    <jats:italic toggle=\"yes\">floR<\/jats:italic>\n                    ). This study indicated the widespread occurrence of MDR ESBLs-\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    in dairy cattle farms. AMR surveillance of more dairy farms and identification of farm-level risk factors are important to mitigate the occurrence and spread of ARB of significant public health importance, such as ESBLs-\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1089\/fpd.2021.0101","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T11:26:10Z","timestamp":1650626770000},"page":"408-416","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":25,"title":["Prevalence of Antimicrobial Resistant and Extended-Spectrum Beta-Lactamase-producing\n                    <i>Escherichia coli<\/i>\n                    in Dairy Cattle Farms in East Tennessee"],"prefix":"10.1177","volume":"19","author":[{"given":"Benti D.","family":"Gelalcha","sequence":"first","affiliation":[{"name":"Department of Animal Science, The University of Tennessee Institute of Agriculture, Knoxville, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desta B.","family":"Ensermu","sequence":"additional","affiliation":[{"name":"Department of Animal Science, The University of Tennessee Institute of Agriculture, Knoxville, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9903-5283","authenticated-orcid":false,"given":"Getahun E.","family":"Agga","sequence":"additional","affiliation":[{"name":"Food Animal Environmental Systems Research Unit, Agricultural Research Service, United States Department of Agriculture, Bowling Green, Kentucky, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly","family":"Vancuren","sequence":"additional","affiliation":[{"name":"Department of Animal Science, The University of Tennessee Institute of Agriculture, Knoxville, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara E.","family":"Gillespie","sequence":"additional","affiliation":[{"name":"Department of Animal Science, The University of Tennessee Institute of Agriculture, Knoxville, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris H.","family":"D'Souza","sequence":"additional","affiliation":[{"name":"Department of Food Science, The University of Tennessee Institute of Agriculture, Knoxville, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chika C.","family":"Okafor","sequence":"additional","affiliation":[{"name":"Department of Biomedical &amp; Diagnostic Sciences, College of Veterinary Medicine, The University of Tennessee, Knoxville, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2108-8905","authenticated-orcid":false,"given":"Oudessa","family":"Kerro Dego","sequence":"additional","affiliation":[{"name":"Department of Animal Science, The University of Tennessee Institute of Agriculture, Knoxville, Tennessee, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,6,10]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11010131"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02430-17"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2015.0259"},{"key":"e_1_3_4_5_1","doi-asserted-by":"crossref","unstructured":"Agga GE Silva PJ Martin RS. 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