{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T06:36:46Z","timestamp":1791441406947,"version":"4.3.3"},"reference-count":38,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:p>\n                    The quinolone ciprofloxacin is a broad-spectrum bactericidal antibiotic used for human medicine as well as the aquaculture industry. The emergence of ciprofloxacin-resistant\n                    <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                    strains is currently a global public health concern. However, the mechanism of ciprofloxacin resistance in\n                    <jats:italic toggle=\"yes\">V. parahaemolyticus<\/jats:italic>\n                    is not yet fully clarified. We generated mutants with decreased ciprofloxacin susceptibility using\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    selection and investigated genes associated with ciprofloxacin resistance on a genetic level. Our selection process yielded mutants that possessed altered minimal inhibitory concentrations (MICs) for ciprofloxacin and other unrelated antibiotics. These included Ser83Ile mutations in GyrA and Val461Glu in ParE as well as mutations in the resistance nodulation cell division (RND) family transporter gene\n                    <jats:italic toggle=\"yes\">vmeD<\/jats:italic>\n                    and the putative TetR family regulator gene\n                    <jats:italic toggle=\"yes\">vp0040<\/jats:italic>\n                    upstream of the\n                    <jats:italic toggle=\"yes\">vmeCD<\/jats:italic>\n                    operon. Measurements of steady-state mRNA levels revealed that the ciprofloxacin-resistant mutants overexpressed\n                    <jats:italic toggle=\"yes\">vmeCD<\/jats:italic>\n                    . Further, the introduction of the\n                    <jats:italic toggle=\"yes\">vp0040<\/jats:italic>\n                    mutated allele from H512 into the sensitive parental strain increased the MIC for ciprofloxacin 31.25-fold. Taken together, these results indicated that ciprofloxacin resistance in these mutants was due to the quinolone resistance determining region mutation as well as overexpression of\n                    <jats:italic toggle=\"yes\">vmeCD<\/jats:italic>\n                    caused by a loss of\n                    <jats:italic toggle=\"yes\">vp0040<\/jats:italic>\n                    gene repression. This also accounted for the presence of the multidrug resistance phenotype for these mutant strains since RND efflux system can export structurally unrelated antibiotics.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2022.0015","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T09:36:01Z","timestamp":1655804161000},"page":"543-549","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Genomic Characterization of Ciprofloxacin Resistance in Laboratory-Derived Mutants of\n                    <i>Vibrio parahaemolyticus<\/i>"],"prefix":"10.1177","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5984-6380","authenticated-orcid":false,"given":"Hai","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfu","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Food Quality and Safety, School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2022,8,11]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006804"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084068"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02008-12"},{"key":"e_1_3_3_5_1","volume-title":"Performance standards for antimicrobial susceptibility testing: Twenty-sixth informational supplement","author":"CLSI (Clinical and Laboratory Standards Institute)","year":"2016","unstructured":"CLSI (Clinical and Laboratory Standards Institute). Performance standards for antimicrobial susceptibility testing: Twenty-sixth informational supplement. M100-S26. Wayne, PA: Clinical and Laboratory Standards Institute, 2016."},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/976273"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.11.4649-4654.2000"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2016.02.008"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv410"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1802080115"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg329"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.2.410"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00149-15"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1101.040762"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1054\/drup.1998.0068"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6514a2"},{"key":"e_1_3_3_17_1","first-page":"1029","article-title":"Mutation supply and relative fitness shape the genotypes of ciprofloxacin-resistant Escherichia coli","volume":"34","author":"Huseby DL","year":"2017","unstructured":"Huseby DL, , Pietsch F, , Brandis G, et al. Mutation supply and relative fitness shape the genotypes of ciprofloxacin-resistant Escherichia coli. Mol Biol Evol, 2017; 34:1029\u20131039.","journal-title":"Mol Biol Evol"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12258"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-016-1210-z"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2010.00846.x"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2019.108461"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01114"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00604-9"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00418-13"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2018.2476"},{"key":"e_1_3_3_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01340-20"},{"key":"e_1_3_3_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.100"},{"key":"e_1_3_3_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.23.6694-6697.2000"},{"key":"e_1_3_3_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04940.x"},{"key":"e_1_3_3_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.5.1156"},{"key":"e_1_3_3_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp054"},{"key":"e_1_3_3_32_1","first-page":"75","article-title":"Use of chemicals and biological products in Asian aquaculture and their potential environmental risks: A critical review","volume":"4","author":"Rico A","year":"2012","unstructured":"Rico A, , Satapornvanit K, , Haque MM, et al. Use of chemicals and biological products in Asian aquaculture and their potential environmental risks: A critical review. Rev Fish Sci, 2012; 4:75\u201393.","journal-title":"Rev Fish Sci"},{"key":"e_1_3_3_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02921-08"},{"key":"e_1_3_3_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10020382"},{"key":"e_1_3_3_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02167-15"},{"key":"e_1_3_3_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2014.05.023"},{"key":"e_1_3_3_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2015.08.005"},{"key":"e_1_3_3_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2013.03.004"},{"key":"e_1_3_3_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2019.108629"}],"container-title":["Foodborne Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/fpd.2022.0015","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2022.0015","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2022.0015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:24:30Z","timestamp":1777443870000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/fpd.2022.0015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1089\/fpd.2022.0015"],"URL":"https:\/\/doi.org\/10.1089\/fpd.2022.0015","relation":{},"ISSN":["1535-3141","1556-7125"],"issn-type":[{"value":"1535-3141","type":"print"},{"value":"1556-7125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8]]}}}