{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:25:03Z","timestamp":1792801503629,"version":"4.5.0"},"reference-count":39,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T00:00:00Z","timestamp":1685577600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2023,6]]},"abstract":"\n Microbial contamination in foods could lead to illnesses and substantial losses in both food industry and public health sectors. Rapid detection of microbial hazards (i.e., pathogens, hygiene indicator microorganisms) can accelerate surveillance and diagnostic processes reducing transmission and minimizing undesirable consequences. This study developed a multiplex PCR (m-PCR) for the detection of six common foodborne pathogens and hygiene indicators using specific primers for\n uidA<\/jats:italic>\n of\n Escherichia coli<\/jats:italic>\n ,\n stx2<\/jats:italic>\n of\n Escherichia coli<\/jats:italic>\n O157:H7,\n invA<\/jats:italic>\n of\n Salmonella<\/jats:italic>\n spp.,\n int<\/jats:italic>\n of\n Shigella<\/jats:italic>\n spp.,\n ntrA<\/jats:italic>\n of\n Klebsiella pneumoniae<\/jats:italic>\n , and\n ail<\/jats:italic>\n of\n Yersinia enterocolitica<\/jats:italic>\n and\n Yersinia pseudotuberculosis<\/jats:italic>\n . Sensitivity of the m-PCR was 100 fg or \u223c20 bacterial cells. Each primer set amplified only the targeted strain, and specificity was demonstrated by lack of nonspecific bands with DNA from 12 other bacterial strains. Following ISO 16140-2:2016, the relative limit of detection of the m-PCR was comparable to that of the gold-standard method; however, the processing time was five times faster. The m-PCR was applied to detect the six pathogens in 100 natural samples (50 pork meat and 50 local fermented food samples) and compared to results of the gold-standard method. Positive cultures for\n Klebsiella<\/jats:italic>\n ,\n Salmonella<\/jats:italic>\n , and\n E. coli<\/jats:italic>\n were 66%, 82%, and 88%, respectively, of meat samples and 78%, 26%, and 56%, respectively, of fermented food samples.\n Escherichia coli<\/jats:italic>\n O157:H7,\n Shigella<\/jats:italic>\n , and\n Yersinia<\/jats:italic>\n were not detected in any of the samples by both standard and m-PCR methods. The developed m-PCR assay showed comparable results with the traditional culture technique proving its rapid and reliable detection of six foodborne pathogens and hygiene indicators in food.\n <\/jats:p>","DOI":"10.1089\/fpd.2022.0062","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T11:08:38Z","timestamp":1684926518000},"page":"222-229","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Development and Validation of an Efficient Multiplex PCR Assay for Simultaneous Detection of Six Common Foodborne Pathogens and Hygiene Indicators"],"prefix":"10.1177","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9569-396X","authenticated-orcid":false,"given":"Natharin","family":"Ngamwongsatit","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand."},{"name":"Laboratory of Bacteriology, Veterinary Diagnostic Center, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soraya","family":"Chaturongakul","sequence":"additional","affiliation":[{"name":"Molecular Medical Biosciences Cluster, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratchaneewan","family":"Aunpad","sequence":"additional","affiliation":[{"name":"Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani, Thailand."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,6,15]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2012.1169"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822011000200006"},{"key":"e_1_3_5_4_1","volume-title":"Escherichia coli","author":"AOAC Official Method 2017.01","unstructured":"AOAC Official Method 2017.01. Escherichia coli O157:H7 in Selected Foods 3MTM Molecular Detection Assay (MDA) 2\u2014E. coli O157 (Including H7) Method. First Action 2017. AOAC International: Rockville, MD, USA."},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13213-013-0622-0"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.10-0367"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02833-10"},{"key":"e_1_3_5_8_1","unstructured":"Bureau of Epidemiology Department of Disease Control Ministry of Public Health. Epidemiology of Foodborne Disease. Mueng Nonthaburi Nonthaburi; 2021. Available from: http:\/\/doe.moph.go.th\/surdata\/506wk\/y64\/d02_5264.pdf [Last accessed: September 12 2022]."},{"key":"e_1_3_5_9_1","unstructured":"Centers for Disease Control and Prevention. Timeline for Reporting Cases of E. coli O157 Infection. Atlanta GA; 2020. Available from: https:\/\/www.cdc.gov\/ecoli\/reporting-timeline.html [Last accessed: September 12 2022]."},{"key":"e_1_3_5_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.34.10.2619-2622.1996"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01921-15"},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJMR2015.7495"},{"key":"e_1_3_5_13_1","unstructured":"Feng P Weagant SD Grant MA et al. BAM chapter 4: Enumeration of Escherichia coli and the coliform bacteria. Bacteriological Analytical Manual. Content current as of: October 9 2020. Available from: https:\/\/www.fda.gov\/food\/laboratory-methods-food\/bam-chapter-4-enumeration-escherichia-coli-and-coliform-bacteria [Last accessed: September 20 2022]."},{"key":"e_1_3_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0153561"},{"key":"e_1_3_5_15_1","doi-asserted-by":"publisher","DOI":"10.4315\/JFP-19-520"},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03142-20"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000011006"},{"key":"e_1_3_5_18_1","volume-title":"Yersinia enterocolitica","author":"ISO10273:2017","unstructured":"ISO10273:2017. Microbiology of f the food chain\u2014horizontal method for the detection of pathogenic Yersinia enterocolitica. International Organization for Standardization: Geneva, Switzerland."},{"key":"e_1_3_5_19_1","unstructured":"ISO21567:2004(E). Microbiology of food and animal feeding stuffs-horizontal method for the detection of Shigella spp. First edition 2004-11-01."},{"key":"e_1_3_5_20_1","unstructured":"ISO6579-1:2017(E). Microbiology of the food chain\u2014horizontal method for the detection enumeration and serotyping of Salmonella. First edition 2017-02."},{"key":"e_1_3_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13468"},{"key":"e_1_3_5_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2013.1638"},{"key":"e_1_3_5_23_1","doi-asserted-by":"publisher","DOI":"10.3821\/2\/2224-6614.2341"},{"key":"e_1_3_5_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-017-1123-2"},{"key":"e_1_3_5_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00222"},{"key":"e_1_3_5_26_1","doi-asserted-by":"publisher","DOI":"10.1558\/5\/mmwr.mm6711a3"},{"key":"e_1_3_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2009.05.011"},{"key":"e_1_3_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.11.013"},{"key":"e_1_3_5_29_1","doi-asserted-by":"publisher","DOI":"10.1522\/6\/2475-4714\/1\/3\/00114"},{"key":"e_1_3_5_30_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/239391"},{"key":"e_1_3_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrp.2016.10.002"},{"key":"e_1_3_5_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02376-21"},{"key":"e_1_3_5_33_1","first-page":"1008","article-title":"High level of shiga toxin-producing Escherichia coli and occurrence of stx-negative E. coli O157 from raw meats: characterization of virulence profile and genetic relatedness","volume":"47","author":"Sirikaew S","year":"2016","unstructured":"Sirikaew S, , Sukkua K, , Rattanachuay P, et al. High level of shiga toxin-producing Escherichia coli and occurrence of stx-negative E. coli O157 from raw meats: characterization of virulence profile and genetic relatedness. Southeast Asian J Trop Med Public Health, 2016; 47:1008\u20131019.","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"e_1_3_5_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11050708"},{"key":"e_1_3_5_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-015-0319-0"},{"key":"e_1_3_5_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-014-2257-x"},{"key":"e_1_3_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2011.06.001"},{"key":"e_1_3_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2011.12.014"},{"key":"e_1_3_5_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-4565.2009.00161.x"},{"key":"e_1_3_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2012.11.044"}],"container-title":["Foodborne Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/fpd.2022.0062","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2022.0062","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2022.0062","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:20:28Z","timestamp":1777443628000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/fpd.2022.0062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1089\/fpd.2022.0062"],"URL":"https:\/\/doi.org\/10.1089\/fpd.2022.0062","relation":{},"ISSN":["1535-3141","1556-7125"],"issn-type":[{"value":"1535-3141","type":"print"},{"value":"1556-7125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6]]}}}