{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T13:20:35Z","timestamp":1783948835274,"version":"3.55.0"},"reference-count":32,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    is a major foodborne pathogen that leads to various diseases due to its biofilm and virulence factors. This study aimed to investigate the inhibitory effect of\n                    <jats:italic toggle=\"yes\">2R,3R<\/jats:italic>\n                    -dihydromyricetin (DMY), a natural flavonoid compound, on the biofilm formation and virulence of\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    , and to explore the mode of action using transcriptomic and proteomic analyses. Microscopic observation revealed that DMY could remarkably inhibit the biofilm formation by\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    , leading to a collapse on the biofilm architecture and a decrease in viability of biofilm cell. Moreover, the hemolytic activity of\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    was reduced to 32.7% after treatment with subinhibitory concentration of DMY (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.01). Bioinformation analysis based on RNA-sequencing and proteomic profiling revealed that DMY induced 262 differentially expressed genes and 669 differentially expressed proteins (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Many downregulated genes and proteins related to surface proteins were involved in biofilm formation, including clumping factor A (ClfA), iron-regulated surface determinants (IsdA, IsdB, and IsdC), fibrinogen-binding proteins (FnbA, FnbB), and serine protease. Meanwhile, DMY regulated a wide range of genes and proteins enriched in bacterial pathogenesis, cell envelope, amino acid metabolism, purine and pyrimidine metabolism, and pyruvate metabolism. These findings suggest that DMY targets\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    through multifarious mechanisms, and especially prompt that interference of surface proteins in cell envelope would lead to attenuation of biofilm and virulence.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2022.0075","type":"journal-article","created":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T10:42:05Z","timestamp":1677753725000},"page":"90-99","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":8,"title":["Antibacterial Mechanism of\n                    <i>2R,3R<\/i>\n                    -Dihydromyricetin Against\n                    <i>Staphylococcus aureus<\/i>\n                    : Deciphering Inhibitory Effect on Biofilm and Virulence Based on Transcriptomic and Proteomic Analyses"],"prefix":"10.1177","volume":"20","author":[{"given":"Wenyi","family":"Ran","sequence":"first","affiliation":[{"name":"Department of Food Engineering, College of Biomass Science and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxi","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Food Engineering, College of Biomass Science and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiwu","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Hygienic Toxicology and Pathology, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China."},{"name":"Research Center for Nutrition, Metabolism and Food Safety, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Food Engineering, College of Biomass Science and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinrong","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Hygienic Toxicology and Pathology, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China."},{"name":"Research Center for Nutrition, Metabolism and Food Safety, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Hygienic Toxicology and Pathology, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China."},{"name":"Research Center for Nutrition, Metabolism and Food Safety, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Bu","sequence":"additional","affiliation":[{"name":"Department of Hygienic Toxicology and Pathology, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China."},{"name":"Research Center for Nutrition, Metabolism and Food Safety, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yina","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Hygienic Toxicology and Pathology, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China."},{"name":"Research Center for Nutrition, Metabolism and Food Safety, West China-PUMC C.C. Chen Institute of Health, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5505-1081","authenticated-orcid":false,"given":"Yanping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Food Engineering, College of Biomass Science and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Food Engineering, College of Biomass Science and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"e_1_3_5_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c8ra09136f"},{"key":"e_1_3_5_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-021-02348-2"},{"key":"e_1_3_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2020.126555"},{"key":"e_1_3_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit668"},{"key":"e_1_3_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11947-020-02521-x"},{"key":"e_1_3_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2013.08.008"},{"key":"e_1_3_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2016.08.001"},{"key":"e_1_3_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-020-10589-w"},{"key":"e_1_3_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2020.113013"},{"key":"e_1_3_5_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000556"},{"key":"e_1_3_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-021-02624-9"},{"key":"e_1_3_5_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1616805114"},{"key":"e_1_3_5_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02515.x"},{"key":"e_1_3_5_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01779"},{"key":"e_1_3_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0730-x"},{"key":"e_1_3_5_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1972280"},{"key":"e_1_3_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.635"},{"key":"e_1_3_5_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.042234-0"},{"key":"e_1_3_5_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja104533w"},{"key":"e_1_3_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.09.008"},{"key":"e_1_3_5_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032523999"},{"key":"e_1_3_5_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02263"},{"key":"e_1_3_5_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01542-14"},{"key":"e_1_3_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.103543"},{"key":"e_1_3_5_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116945"},{"key":"e_1_3_5_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-021-02961-3"},{"key":"e_1_3_5_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-015-1905-3"},{"key":"e_1_3_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2009.12.008"},{"key":"e_1_3_5_30_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.10671"},{"key":"e_1_3_5_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.48"},{"key":"e_1_3_5_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2017.2405"},{"key":"e_1_3_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.07.090"}],"container-title":["Foodborne Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/fpd.2022.0075","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2022.0075","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2022.0075","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:20:25Z","timestamp":1777443625000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/fpd.2022.0075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1089\/fpd.2022.0075"],"URL":"https:\/\/doi.org\/10.1089\/fpd.2022.0075","relation":{},"ISSN":["1535-3141","1556-7125"],"issn-type":[{"value":"1535-3141","type":"print"},{"value":"1556-7125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3]]}}}