{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:14:48Z","timestamp":1779351288610,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T00:00:00Z","timestamp":1709251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Cronobacter sakazakii<\/jats:italic>\n                    , an opportunistic milk-borne pathogen responsible for severe neonatal meningitis and bacteremia, can synthesize yellow pigment (various carotenoids) benefiting for bacterial survival, while little literature was available about the influence of various carotenoids on bacterial resistance to a series of stresses and the characteristics of cell membrane, obstructing the development of novel bactericidal strategies overcoming the strong tolerance of\n                    <jats:italic toggle=\"yes\">C. sakazakii<\/jats:italic>\n                    . Thus in this study, for the first time, five carotenogenic genes of\n                    <jats:italic toggle=\"yes\">C. sakazakii<\/jats:italic>\n                    BAA-894 were inactivated, respectively, to construct a series of mutants producing various carotenoids and their effects on the cell membrane properties, and resistances to food- and host-related stresses, were investigated systematically. Furthermore, to explore its possible mode of action, comparative lipidomics analysis was performed to reveal the change of lipids that were mainly located at cell membranes. The results showed that five mutants (\u0394\n                    <jats:italic toggle=\"yes\">crtB<\/jats:italic>\n                    , \u0394\n                    <jats:italic toggle=\"yes\">crtI<\/jats:italic>\n                    , \u0394\n                    <jats:italic toggle=\"yes\">crtY<\/jats:italic>\n                    , \u0394\n                    <jats:italic toggle=\"yes\">crtZ<\/jats:italic>\n                    , and \u0394\n                    <jats:italic toggle=\"yes\">crtX<\/jats:italic>\n                    ) displayed negligible change in growth rate but higher permeability of the outer membrane and lower fluidity of cell membrane compared to the wild type. Besides, these mutants exhibited poorer ability of biofilm formation and lower resistances to acid, oxidative, osmotic, and desiccation stresses, indicating that different carotenoid composition significantly affected environmental tolerance of\n                    <jats:italic toggle=\"yes\">C. sakazakii<\/jats:italic>\n                    . To discover the possible causes, lipidomics analysis of\n                    <jats:italic toggle=\"yes\">C. sakazakii<\/jats:italic>\n                    was conducted and more than 500 lipid species belonging to 27 classes had been identified at first. Compared to that of BAA-894, the composition and relative intensity of lipid species in five mutants varied significantly, especially the monounsaturated and biunsaturated phosphatidylethanolamine. The evidence presented in this study demonstrated that the varied composition of carotenoids in\n                    <jats:italic toggle=\"yes\">C. sakazakii<\/jats:italic>\n                    significantly altered the lipid profile and intensity, which maybe a crucial means to influencing the characteristics of cell membranes and resistance to environmental stresses.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2023.0022","type":"journal-article","created":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T11:04:02Z","timestamp":1702983842000},"page":"174-182","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Inactivation of Carotenogenic-Biosynthesizing Genes Altered Lipids Composition and Intensity in\n                    <i>Cronobacter sakazakii<\/i>"],"prefix":"10.1177","volume":"21","author":[{"given":"Xi","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuyan","family":"Wu","sequence":"additional","affiliation":[{"name":"Hopkirk Research Institute, AgResearch Ltd., Palmerston North, New Zealand."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuanghua","family":"Luo","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Weng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodong","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangzhou YueHui Cosmetics Co. Ltd., Guangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, China."},{"name":"International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4468-2889","authenticated-orcid":false,"given":"Xiaoqing","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Food Science and Technology, School of Biotechnology, Jiangnan University, Wuxi, China."},{"name":"Hopkirk Research Institute, AgResearch Ltd., Palmerston North, New Zealand."},{"name":"International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,3,6]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-39126-7_1"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.2179"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.41.100187.002251"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02867"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9112338"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2004.11.015"},{"key":"e_1_3_4_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2005.02.013"},{"key":"e_1_3_4_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365518809085746"},{"key":"e_1_3_4_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2000.00971.x"},{"key":"e_1_3_4_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2011.02436.x"},{"key":"e_1_3_4_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01420-09"},{"key":"e_1_3_4_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/c7cp07126d"},{"key":"e_1_3_4_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.01740-07"},{"key":"e_1_3_4_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00500.x"},{"key":"e_1_3_4_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/d0cp01031f"},{"key":"e_1_3_4_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2007.05.001"},{"key":"e_1_3_4_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0168-1605(96)01172-5"},{"key":"e_1_3_4_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00797.x"},{"key":"e_1_3_4_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2017.1313372"},{"key":"e_1_3_4_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2008.04.013"},{"key":"e_1_3_4_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.12.8141-8146.2005"},{"key":"e_1_3_4_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-57006-5"},{"key":"e_1_3_4_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuv008"},{"key":"e_1_3_4_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/md13063325"},{"key":"e_1_3_4_28_1","doi-asserted-by":"publisher","DOI":"10.1721\/9\/acem\/31243"},{"key":"e_1_3_4_29_1","doi-asserted-by":"publisher","DOI":"10.1322\/7\/j.hjkx.201609001"},{"key":"e_1_3_4_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086739"},{"key":"e_1_3_4_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.151403"}],"container-title":["Foodborne Pathogens and Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/fpd.2023.0022","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2023.0022","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/fpd.2023.0022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:20:40Z","timestamp":1777443640000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/fpd.2023.0022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1089\/fpd.2023.0022"],"URL":"https:\/\/doi.org\/10.1089\/fpd.2023.0022","relation":{},"ISSN":["1535-3141","1556-7125"],"issn-type":[{"value":"1535-3141","type":"print"},{"value":"1556-7125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3]]}}}