{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T14:04:32Z","timestamp":1789567472478,"version":"build-2803163510"},"reference-count":33,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2024,3,22]],"date-time":"2024-03-22T00:00:00Z","timestamp":1711065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    is a globally prevalent foodborne bacterium, and ceftriaxone and azithromycin have been regarded as drugs of choice for treating\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    infections, particularly in children. With the growing incidence of ceftriaxone and azithromycin resistance in\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    , there is an urgent requirement for a rapid and dependable gene testing approach to enhance the efficacy of treating\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    infections. Utilizing the orange to green visible dye approach, this study developed loop-mediated isothermal amplification (LAMP) assays for the sensitive and specific detection of\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    , ceftriaxone and azithromycin resistance genes (including CTX-M-1 group,\n                    <jats:italic toggle=\"yes\">mph(A)<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                    genes) in stool and blood samples. The specificity and sensitivity of primers during the LAMP assays for detection of\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    , CTX-M-1 group,\n                    <jats:italic toggle=\"yes\">mph(A)<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                    genes were determined in this study. The detection threshold for\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    was found to be 1.5\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    colony-forming units (CFU)\/mL, while it was 1.5\u2009\u00d7\u200910\n                    <jats:sup>2<\/jats:sup>\n                    CFU\/mL for CTX-M-1 group genes (including\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-3<\/jats:sub>\n                    ,\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    , and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-55<\/jats:sub>\n                    ), 1.5\u2009\u00d7\u200910\n                    <jats:sup>2<\/jats:sup>\n                    CFU\/mL for\n                    <jats:italic toggle=\"yes\">mph(A)<\/jats:italic>\n                    , and 1.5\u2009\u00d7\u200910\n                    <jats:sup>2<\/jats:sup>\n                    CFU\/mL for\n                    <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                    , showing 10\u201310\n                    <jats:sup>3<\/jats:sup>\n                    -fold, 10\n                    <jats:sup>3<\/jats:sup>\n                    -fold, and 10\n                    <jats:sup>5<\/jats:sup>\n                    -fold increased sensitivity compared with the polymerase chain reaction assay, respectively. Results indicated that the LAMP primers designed for\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    , CTX-M-1 group,\n                    <jats:italic toggle=\"yes\">mph(A)<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                    genes possess high specificity (100%) and sensitivity (over 94%). This novel approach advocates its application in detecting\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    , CTX-M-1 group,\n                    <jats:italic toggle=\"yes\">mph(A)<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                    genes.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2023.0094","type":"journal-article","created":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T14:30:22Z","timestamp":1711377022000},"page":"58-65","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Sensitive and Specific Loop-Mediated Isothermal Amplification Assays for Detection of\n                    <i>Salmonella<\/i>\n                    , CTX-M-1 Group Genes,\n                    <i>mph(A)<\/i>\n                    , and\n                    <i>ermB<\/i>\n                    in Stool and Blood Samples Based on Orange to Green Visible Dye"],"prefix":"10.1177","volume":"22","author":[{"given":"Jianbo","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, DongGuan SongShan Lake Tungwah Hospital, Dongguan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, DongGuan SongShan Lake Tungwah Hospital, Dongguan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huixin","family":"Chen","sequence":"additional","affiliation":[{"name":"Technical Support Department, Guangzhou Haoyang Human Resources Co., Ltd., GuangZhou, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujin","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, DongGuan Tungwah Hospital, Dongguan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congzhu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, DongGuan SongShan Lake Tungwah Hospital, Dongguan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weixin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, DongGuan SongShan Lake Tungwah Hospital, Dongguan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinglin","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, DongGuan SongShan Lake Tungwah Hospital, Dongguan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentao","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, DongGuan SongShan Lake Tungwah Hospital, Dongguan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajie","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, DongGuan SongShan Lake Tungwah Hospital, Dongguan, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9668-523X","authenticated-orcid":false,"given":"Fei","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, DongGuan SongShan Lake Tungwah Hospital, Dongguan, 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