{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:50:54Z","timestamp":1792155054359,"version":"4.4.0"},"reference-count":69,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:p>\n                    Members of the\n                    <jats:italic toggle=\"yes\">Bacillus cereus<\/jats:italic>\n                    group are well-known opportunistic foodborne pathogens. In this study, the prevalence, hemolytic activity, antimicrobial resistance profile, virulence factor genes, genetic diversity by enterobacterial repetitive intergenic consensus (ERIC)-polymerase chain reaction (PCR) genotyping, and adhesion potential were investigated in isolates from a Tunisian dairy farm environment and raw milk. A total of 200 samples, including bedding, feces, feed, liquid manure, and raw bovine milk, were examined. Based on PCR test targeting\n                    <jats:italic toggle=\"yes\">sspE<\/jats:italic>\n                    gene, 59 isolates were detected. The prevalence of\n                    <jats:italic toggle=\"yes\">B. cereus<\/jats:italic>\n                    group isolates in bedding, feces, liquid manure, feed, and raw milk was 48%, 37.8%, 20%, 17.1%, and 12.5%, respectively. Out of the tested strains, 81.4% showed \u03b2-hemolytic on blood agar plates. An antimicrobial resistance test against 11 antibiotics showed that more than 50% of the isolates were resistant to ampicillin and novobiocin, while a high sensitivity to other antibiotics tested was observed in most isolates. The distribution of enterotoxigenic genes showed that 8.5% and 67.8% of isolates carried\n                    <jats:italic toggle=\"yes\">hblABCD<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">nheABC<\/jats:italic>\n                    , respectively. In addition, the detection rate of cytotoxin K (\n                    <jats:italic toggle=\"yes\">cytk<\/jats:italic>\n                    ), enterotoxin T (\n                    <jats:italic toggle=\"yes\">bceT<\/jats:italic>\n                    ), and\n                    <jats:italic toggle=\"yes\">ces<\/jats:italic>\n                    genes was 72.9%, 64.4%, and 5.1%, respectively. ERIC-PCR fingerprinting genotype analysis allowed discriminating 40 different profiles. The adhesion potential of\n                    <jats:italic toggle=\"yes\">B. cereus<\/jats:italic>\n                    group on stainless steel showed that all isolates were able to adhere at various levels, from 1.5\u2009\u00b1\u20090.3 to 5.1\u2009\u00b1\u20090.1 log colony-forming unit (CFU)\/cm\n                    <jats:sup>2<\/jats:sup>\n                    for vegetative cells and from 2.6\u2009\u00b1\u20090.4 to 5.7\u2009\u00b1\u20090.3 log CFU\/cm\n                    <jats:sup>2<\/jats:sup>\n                    for spores. An important finding of the study is useful for updating the knowledge of the contamination status of\n                    <jats:italic toggle=\"yes\">B. cereus<\/jats:italic>\n                    group in Tunisia, at the dairy farm level.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2023.0154","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T14:35:15Z","timestamp":1710858915000},"page":"263-272","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Isolation, Identification, and Characterization of\n                    <i>Bacillus cereus<\/i>\n                    Group Bacteria Isolated from the Dairy Farm Environment and Raw Milk in Tunisia"],"prefix":"10.1177","volume":"22","author":[{"given":"Randa","family":"Ben Akacha","sequence":"first","affiliation":[{"name":"Research Laboratory of Environmental Toxicology Microbiology and Health (LR17ES06), Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia."},{"name":"Equipe Microbiologie de l'\u0152uf et des Ovoproduits (MICOV), Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l'\u0152uf, Rennes, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4482-2869","authenticated-orcid":false,"given":"Maroua","family":"Gdoura-Ben Amor","sequence":"additional","affiliation":[{"name":"Research Laboratory of Environmental Toxicology Microbiology and Health (LR17ES06), Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia."},{"name":"Equipe Microbiologie de l'\u0152uf et des Ovoproduits (MICOV), Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l'\u0152uf, Rennes, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanen","family":"Sellami","sequence":"additional","affiliation":[{"name":"Laboratory of Treatment and Valorization of Water Rejects, Water Research and Technologies Center (CERTE), Borj-Cedria Technopark, University of Carthage, Soliman, Tunisia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00ebl","family":"Grosset","sequence":"additional","affiliation":[{"name":"Equipe Microbiologie de l'\u0152uf et des Ovoproduits (MICOV), Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l'\u0152uf, Rennes, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Jan","sequence":"additional","affiliation":[{"name":"Equipe Microbiologie de l'\u0152uf et des Ovoproduits (MICOV), Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l'\u0152uf, Rennes, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Gautier","sequence":"additional","affiliation":[{"name":"Equipe Microbiologie de l'\u0152uf et des Ovoproduits (MICOV), Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l'\u0152uf, Rennes, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radhouane","family":"Gdoura","sequence":"additional","affiliation":[{"name":"Research Laboratory of Environmental Toxicology Microbiology and Health (LR17ES06), Faculty of Sciences of Sfax, University of Sfax, Sfax, 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