{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T10:08:02Z","timestamp":1778839682439,"version":"3.51.4"},"reference-count":26,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2025,11,1]]},"abstract":"<jats:p>\n                    PrfA is a key virulence regulator for\n                    <jats:italic toggle=\"yes\">Listeria monocytogenes<\/jats:italic>\n                    (Lm) responding to host environment. Here we report that the natural mutation in PrfA\n                    <jats:sub>K10N\/T151A<\/jats:sub>\n                    enhanced the pathogenicity of hypervirulent serotype 4h\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    . We characterized the phylogenetic tree of PrfA, and found that PrfA\n                    <jats:sub>K10N\/T151A<\/jats:sub>\n                    prevalently distributed in all serotype 4h isolates. Remarkably, the growth rate of serotype 4h strain Lm XYSN was significantly slower than EGD-e, in contrast, the substitution mutant Lm PrfA\n                    <jats:sub>N10K\/A151T<\/jats:sub>\n                    increased the growth rate of\n                    <jats:italic toggle=\"yes\">L. monocytogenes<\/jats:italic>\n                    . Notably, PrfA\n                    <jats:sub>K10N\/T151A<\/jats:sub>\n                    upregulated the expression of multiple virulent genes of Lm XYSN cultured in brain-heart infusion medium, and increased the invasion ability in HeLa and Caco-2 cells. Importantly, the PrfA\n                    <jats:sub>K10N\/T151A<\/jats:sub>\n                    mutation significantly enhanced the colonization and survival of Lm XYSN\n                    <jats:italic toggle=\"yes\">in\u00a0vivo<\/jats:italic>\n                    . Therefore, our findings indicate that the natural mutation of PrfA\n                    <jats:sub>K10N\/T151A<\/jats:sub>\n                    enhances the PrfA activity, resulting in the upregulation of various virulence genes and contributing to virulence and pathogenesis of Lm XYSN, which contributes to the hypervirulence of serotype 4h isolates.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2024.0050","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T12:08:32Z","timestamp":1736165312000},"page":"789-797","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Natural Mutation of PrfA K10N\/T151A Enhances Serotype 4h\n                    <i>Listeria monocytogenes<\/i>\n                    Virulence"],"prefix":"10.1177","volume":"22","author":[{"given":"Jing","family":"Li","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China."},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, Yangzhou University, Yangzhou, China."},{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China."},{"name":"College of Biological Sciences and Technology, Yangzhou University, Yangzhou, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuexue","family":"Cai","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China."},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, Yangzhou University, Yangzhou, 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