{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:53:10Z","timestamp":1780494790880,"version":"3.54.1"},"reference-count":39,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:00:00Z","timestamp":1741305600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Foodborne Pathogens and Disease"],"published-print":{"date-parts":[[2026,3,1]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    spp. is a leading cause of community-acquired gastroenteritis, with rising antimicrobial resistance (AMR) posing a significant public health challenge. This study aimed to characterize the genomic basis of resistance and virulence in\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    spp. isolated from human stool at the University Hospital of Split, Croatia. A total of 115 unduplicated isolates (100\n                    <jats:italic toggle=\"yes\">Campylobacter jejuni<\/jats:italic>\n                    and 15\n                    <jats:italic toggle=\"yes\">Campylobacter coli<\/jats:italic>\n                    ) were identified and tested for antimicrobial susceptibility, from January to June 2023. A representative number of isolates with phenotypically detected resistance were analyzed with whole-genome sequencing. Multilocus sequence typing (MLST) was determined using genomic sequences as input data against the standard MLST schemes. High rates of resistance were detected to tetracycline (45% of\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    and 26.7% of\n                    <jats:italic toggle=\"yes\">C. coli<\/jats:italic>\n                    ) and ciprofloxacin (81% of\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    and 46.7% of\n                    <jats:italic toggle=\"yes\">C. coli<\/jats:italic>\n                    ), while combined ciprofloxacin\/tetracycline resistance was observed in 43% of\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    and 7% of\n                    <jats:italic toggle=\"yes\">C. coli<\/jats:italic>\n                    isolates. The majority of whole-genome sequenced isolates possess\n                    <jats:italic toggle=\"yes\">tet(O\/32\/O)<\/jats:italic>\n                    gene and\n                    <jats:italic toggle=\"yes\">gyrA.T861<\/jats:italic>\n                    mutation, conferring resistance to tetracycline and fluoroquinolones. All isolates have at least one resistance gene for \u03b2-lactams (\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-61<\/jats:sub>\n                    ,\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-193<\/jats:sub>\n                    ,\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-450<\/jats:sub>\n                    ,\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-451<\/jats:sub>\n                    ,\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-452<\/jats:sub>\n                    ,\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-461<\/jats:sub>\n                    , and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>OXA-489<\/jats:sub>\n                    ), while 40% of isolates possess\n                    <jats:italic toggle=\"yes\">ant(6)-Ia<\/jats:italic>\n                    for aminoglycoside resistance. In addition, a newly emerged, phenotypically identified multidrug-resistant\n                    <jats:italic toggle=\"yes\">C. coli<\/jats:italic>\n                    , harbored\n                    <jats:italic toggle=\"yes\">tet(O\/32\/O)<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">tet(O)<\/jats:italic>\n                    genes, point mutation in\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    gene, and A2075G mutation in 23S rRNA. Virulence analysis highlighted\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    \u2019s broad pathogenic potential, including motility, adherence, invasion, exotoxin production, and immune modulation (e.g.,\n                    <jats:italic toggle=\"yes\">wlaN<\/jats:italic>\n                    gene, linked to Guillain\u2013Barr\u00e9 syndrome). On the contrary,\n                    <jats:italic toggle=\"yes\">C. coli<\/jats:italic>\n                    isolates possess genes exclusively for motility. Nine different\n                    <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                    MLST sequence types were identified, while all\n                    <jats:italic toggle=\"yes\">C. coli<\/jats:italic>\n                    isolates belong to the same 828 clonal complex. Escalating AMR and a broad spectrum of virulence in\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    spp. highlight the importance of continuous surveillance on the phenotypic and genotypic level, thereby allowing more efficient health care management of these infections.\n                  <\/jats:p>","DOI":"10.1089\/fpd.2024.0173","type":"journal-article","created":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T11:21:42Z","timestamp":1741346502000},"page":"189-200","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Phenotypic and Whole-Genome Sequencing-Based Profiling of Antimicrobial Resistance and Virulence in\n                    <i>Campylobacter<\/i>\n                    spp. Isolated from Human Stool in a Croatian University Hospital"],"prefix":"10.1177","volume":"23","author":[{"given":"Luka","family":"Katic","sequence":"first","affiliation":[{"name":"Department of Medicine, Icahn School of Medicine at Mount Sinai Morningside\/West, New York, New York, USA."},{"name":"ESCMID Food- and Water-borne Infections Study Group (EFWISG), Basel, Switzerland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Feren\u010dak","sequence":"additional","affiliation":[{"name":"ESCMID Food- and Water-borne Infections Study Group (EFWISG), Basel, Switzerland."},{"name":"Croatian Institute for Public Health, Zagreb, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Gveri\u0107-Grgini\u0107","sequence":"additional","affiliation":[{"name":"ESCMID Food- and Water-borne Infections Study Group (EFWISG), Basel, Switzerland."},{"name":"Croatian Institute for Public Health, Zagreb, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Goic-Barisic","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital of Split, Split, Croatia."},{"name":"University of Split, School of Medicine Split, Split, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zana","family":"Rubic","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital of Split, Split, Croatia."},{"name":"University of Split, School of Medicine Split, Split, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Radic-Skelin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital of Split, Split, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Marinovic","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital of Split, Split, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dora","family":"Smoli\u0107","sequence":"additional","affiliation":[{"name":"Croatian Institute for Public Health, Zagreb, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josipa","family":"Kuzle","sequence":"additional","affiliation":[{"name":"Croatian Institute for Public Health, Zagreb, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucija","family":"\u0160kara Abramovi\u0107","sequence":"additional","affiliation":[{"name":"Croatian Institute for Public Health, Zagreb, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irena","family":"Tabain","sequence":"additional","affiliation":[{"name":"Croatian Institute for Public Health, Zagreb, Croatia."},{"name":"University of Split, School of Medicine Split, Split, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragan","family":"Juri\u0107","sequence":"additional","affiliation":[{"name":"Croatian Institute for Public Health, Zagreb, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Tonkic","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University Hospital of Split, Split, Croatia."},{"name":"University of Split, School of Medicine Split, Split, Croatia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgina","family":"Osorio","sequence":"additional","affiliation":[{"name":"Department of Medicine, Icahn School of Medicine at Mount Sinai Morningside\/West, New York, New York, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6330-4058","authenticated-orcid":false,"given":"Anita","family":"Novak","sequence":"additional","affiliation":[{"name":"ESCMID Food- and Water-borne Infections 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