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This monitoring maintains optimal conditions, reduces waste, and enables swift corrective actions, minimizing the risk of producing out-of-specification products. With advancements in cell-free biomanufacturing, the importance of in-line sensing technologies has increased, as they provide real-time tracking of biochemical processes, allowing immediate adjustments to keep manufacturing efficient and consistent. In this study, we successfully developed a graphene field-effect transistor (gFET) sensor to monitor key parameters such as glucose, pH, and immunoglobulin G antibody levels in cell culture media from a CHO cells bioreactor. The gFET sensor accurately detected pH levels between 6.8 and 8.2, glucose concentrations from 5 to 30 mM, and antibody levels ranging from 25 to 100 \u00b5g\/mL, highlighting the potential of graphene sensors for inline sensing in advanced biomanufacturing.<\/jats:p>","DOI":"10.1089\/genbio.2024.0043","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T12:30:43Z","timestamp":1733488243000},"page":"341-349","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Real-Time Monitoring in Biomanufacturing with Graphene Field-Effect Transistor Sensors: Detection of pH, Glucose, and Antibodies"],"prefix":"10.1177","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7662-3675","authenticated-orcid":false,"given":"Deependra K.","family":"Ban","sequence":"first","affiliation":[{"name":"Shu Chien-Gene Lay Department of Bioengineering, Jacobs School of Engineering Department of Bioengineering, University of California, San Diego, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Hajian","sequence":"additional","affiliation":[{"name":"School of Applied Biosciences, Keck Graduate Institute, Claremont, California, USA."},{"name":"Rapid Diagnostics Division, Abbott Laboratories, Pomona, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiva","family":"Abdolrahimi","sequence":"additional","affiliation":[{"name":"Amgen Bioprocessing Center, Keck Graduate Institute, Claremont, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francie","family":"Barron","sequence":"additional","affiliation":[{"name":"Cardea Bio Inc., San Diego, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurav","family":"Datta","sequence":"additional","affiliation":[{"name":"Amgen Bioprocessing Center, Keck Graduate Institute, Claremont, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiana","family":"Aran","sequence":"additional","affiliation":[{"name":"Shu Chien-Gene Lay Department of Bioengineering, Jacobs School of Engineering Department of Bioengineering, University of California, San Diego, California, USA."},{"name":"Cardea Bio Inc., San Diego, California, USA."},{"name":"Department of Medicine, Division of Geriatrics, Gerontology &amp; Palliative Care, School of Medicine, University of California, San Diego, California, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,12,13]]},"reference":[{"issue":"6","key":"e_1_3_6_2_2","article-title":"Bioprocess control: Current progress and future perspectives","volume":"11","author":"Rathore AS","year":"2021","unstructured":"Rathore AS, , Mishra S, , Nikita S, et al. 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