{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T19:39:14Z","timestamp":1781206754487,"version":"3.54.1"},"reference-count":50,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T00:00:00Z","timestamp":1462924800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Genetic Testing and Molecular Biomarkers"],"published-print":{"date-parts":[[2016,6,1]]},"abstract":"\n Objective:<\/jats:italic>\n To examine the associations of\n methionine synthase<\/jats:italic>\n (\n MTR<\/jats:italic>\n ),\n methionine synthase reductase<\/jats:italic>\n (\n MTRR<\/jats:italic>\n ), and\n methylenetetrahydrofolate reductase<\/jats:italic>\n (\n MTHFR<\/jats:italic>\n ) gene polymorphisms with the susceptibility to nonsyndromic cleft lip with or without cleft palate (NSCL\/P).\n Methods:<\/jats:italic>\n Between May 2012 and August 2014, 147 NSCL\/P patients (case group) and 129 healthy volunteers (control group) were recruited for the study. The\n MTR<\/jats:italic>\n A2756G,\n MTRR<\/jats:italic>\n A66G,\n MTHFR<\/jats:italic>\n C677T and\n MTHFR<\/jats:italic>\n A1298C polymorphisms were assessed by polymerase chain reaction-restriction fragment length polymorphism. Haplotype analyses were performed with SHEsis software. Logistic regression analysis was used to evaluate the possible risk factors for NSCL\/P. Generalized multifactor dimensionality reduction (GMDR) was applied to detect gene-gene interactions.\n Results:<\/jats:italic>\n MTR<\/jats:italic>\n A2756G,\n MTRR<\/jats:italic>\n A66G, and\n MTHFR<\/jats:italic>\n C677T gene polymorphisms were associated with the risk of NSCL\/P (all\n p<\/jats:italic>\n \u2009<\u20090.05). Logistic regression analysis revealed that\n MTR<\/jats:italic>\n A2756G,\n MTR<\/jats:italic>\n RA66G, and\n MTHFR<\/jats:italic>\n C667T might increase the risk of NSCL\/P (odds ratio [OR]\u2009=\u20090.270, 95% confidence interval [95% CI]\u2009=\u20090.106-0.689; OR\u2009=\u20090.159, 95% CI\u2009=\u20090.069-0.368; OR\u2009=\u20090.343, 95% CI\u2009=\u20090.139-0.844). The CA haplotype in the\n MTHFR<\/jats:italic>\n gene may serve as a protective factor for NSCL\/P (OR\u2009=\u20090.658, 95% CI\u2009=\u20090.470-0.923), and the TA haplotype might be a risk factor (OR\u2009=\u20092.001, 95% CI\u2009=\u20091.301-3.077). GMDR revealed that the optimal models were two- and four-dimensional models with prediction accuracies of 75.73% (\n p<\/jats:italic>\n \u2009=\u20090.001) and 77.21% (\n p<\/jats:italic>\n \u2009=\u20090.001) and the best cross-validation consistencies of 10\/10 and 10\/10, respectively.\n Conclusion:<\/jats:italic>\n MTR<\/jats:italic>\n A2756G,\n MTRR<\/jats:italic>\n A66G, and\n MTHFR<\/jats:italic>\n C677T polymorphisms may be related to NSCL\/P, and interactions were detected between the\n MTR<\/jats:italic>\n A2756G,\n MTRR<\/jats:italic>\n A66G, and\n MTHFR<\/jats:italic>\n C677T and A1298C polymorphisms.\n <\/jats:p>","DOI":"10.1089\/gtmb.2015.0186","type":"journal-article","created":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T13:57:20Z","timestamp":1462975040000},"page":"297-303","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":27,"title":["MTR, MTRR,<\/i>\n and\n MTHFR<\/i>\n Gene Polymorphisms and Susceptibility to Nonsyndromic Cleft Lip With or Without Cleft Palate"],"prefix":"10.1177","volume":"20","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[{"name":"Harbin Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Hui","family":"Jiao","sequence":"additional","affiliation":[{"name":"Harbin Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Harbin Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"Harbin Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Dong","sequence":"additional","affiliation":[{"name":"Harbin Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,5,11]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/gtmb.2008.0115"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2011.01.007"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.333"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-012-1629-5"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20740"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.36141"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.37181"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20732"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2007.06.007"},{"key":"e_1_3_3_11_1","first-page":"51","article-title":"Allelic polymorphism of MTHFR, MTR and MTRR genes in patients with cleft lip and\/or palate and their mothers [in Ukrainian]","volume":"45","author":"Chorna LB","year":"2011","unstructured":"Chorna LB, , Akopian HR, , Makukh HV, et al. 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