{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T11:55:24Z","timestamp":1782561324729,"version":"3.54.5"},"reference-count":29,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Genetic Testing and Molecular Biomarkers"],"published-print":{"date-parts":[[2019,3]]},"abstract":"\n Background:<\/jats:title>\n Epithelial-mesenchymal transition (EMT) of the medial edge epithelium (MEE) occurs through fusion of the palatal shelves and is a crucial step in palatogenesis. The key genes, however, and the related signaling pathway of EMT are not yet fully understood. Therefore, the aim of this study was to reveal the key genes and the related signaling pathway of EMT during palatal fusion.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n C57BL\/6J mice at embryonic gestation day 14.5 (E14.5;\n n<\/jats:italic>\n \u2009=\u20096) were used to establish the cleft palate model for mRNA-Seq (HiSeq X Ten). The Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed for functional annotations of the differentially expressed genes. Quantitative polymerase chain reaction (qPCR) assays were used to validate the RNAseq data.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n A total of 936 differentially expressed genes, including 558 upregulated and 378 downregulated genes were identified in cases versus controls, respectively. Among these genes, the GO analysis showed that Lymphoid Enhancer-Binding Factor 1 (\n LEF1)<\/jats:italic>\n and\n SMAD<\/jats:italic>\n Family Member 3 (\n SMAD3)<\/jats:italic>\n significantly enriched biological processes, which were EMT related. The KEGG analysis showed that these genes regulated EMT through the Hippo signaling pathway.\n LEF1<\/jats:italic>\n and\n SMAD3<\/jats:italic>\n were downregulated, and the qPCR results corroborated the RNA-seq data.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n These results demonstrate that\n LEF1<\/jats:italic>\n and\n SMAD3<\/jats:italic>\n inhibits EMT at the MEE through the Hippo signaling pathway; and that this could contribute to cleft palate formation in embryonic palatal fusion at E 14.5.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1089\/gtmb.2018.0221","type":"journal-article","created":{"date-parts":[[2019,2,15]],"date-time":"2019-02-15T10:05:59Z","timestamp":1550225159000},"page":"197-203","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Genome-Wide mRNA-Seq Profiling Reveals that\n LEF1<\/i>\n and\n SMAD3<\/i>\n Regulate Epithelial-Mesenchymal Transition Through the Hippo Signaling Pathway During Palatal Fusion"],"prefix":"10.1177","volume":"23","author":[{"given":"Xuan","family":"Shu","sequence":"first","affiliation":[{"name":"The Cleft Lip and Palate Treatment Center, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenyou","family":"Shu","sequence":"additional","affiliation":[{"name":"The Cleft Lip and Palate Treatment Center, Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongqiu","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Second Affiliated Hospital of Shantou University Medical College, Shantou, Shantou, Guangdong, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,3,15]]},"reference":[{"key":"e_1_3_4_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1753-4887.2011.00425.x"},{"key":"e_1_3_4_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_3_4_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.114.2.379"},{"key":"e_1_3_4_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00174615"},{"key":"e_1_3_4_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0620"},{"key":"e_1_3_4_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.4.2495"},{"key":"e_1_3_4_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9926(199811)58:5<197::AID-TERA6>3.0.CO;2-8"},{"key":"e_1_3_4_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmg.2013.06.007"},{"key":"e_1_3_4_10_1","doi-asserted-by":"crossref","first-page":"D258","DOI":"10.1093\/nar\/gkh036","article-title":"The Gene Ontology (GO) database and informatics resource","volume":"32","author":"Harris M","year":"2004","unstructured":"Harris M, , Clark J, , Ireland A, et al. 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