{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T18:32:42Z","timestamp":1777660362274,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Genetic Testing and Molecular Biomarkers"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec>\n                    <jats:title>Aims:<\/jats:title>\n                    <jats:p>This study was designed to investigate differentially expressed genes (DEGs) in the annulus fibrosus (AF), nucleus pulposus (NP), and whole blood (WB) of intervertebral disk degeneration (IDD) patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>We retrieved microarray data set GSE70362, which contains the gene expression profiles of 24 AF and 24 NP samples from the Gene Expression Omnibus and identified DEGs in degenerative AF (AF-DEGs) and NP (NP-DEGs) samples compared with nondegenerative samples. We also examined gene expression profiles in WB from patients with IDD and healthy volunteers to identify DEGs in WB (WB-DEGs). We performed functional analyses on the DEGs common to AF-DEGs, NP-DEGs, and WB-DEGs. Expression of the common DEGs was partially validated by quantitative real-time-polymerase chain reaction (QRT-PCR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      In total, 846 AF-DEGs, 902 NP-DEGs, and 862 WB-DEGs were identified, and 22 DEGs were common among the three groups. Functional analyses showed that the common DEGs were enriched in 33 biological processes, 16 cellular components, 4 molecular functions, and 9 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways; 13 of the common DEGs were included in the protein-protein interaction (PPI) network and superoxide dismutase 2 (\n                      <jats:italic toggle=\"yes\">SOD2<\/jats:italic>\n                      ) was identified as a hub gene in the PPI network. The QRT-PCR results for the expression of the genes protein disulfide isomerase family A member 4, FKBP prolyl isomerase 11, ectonucleotide pyrophosphatase\/phosphodiesterase 4,\n                      <jats:italic toggle=\"yes\">SOD2<\/jats:italic>\n                      , and actin binding LIM protein 1, were consistent with the gene chip hybridization results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>This study identified key genes for future investigations of the underlying molecular mechanisms of IDD. These genes may provide future targets for the clinical treatment and diagnosis of IDD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/gtmb.2019.0043","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T11:52:23Z","timestamp":1564660343000},"page":"610-617","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification of Key Genes Potentially Related to Intervertebral Disk Degeneration by Microarray Analysis"],"prefix":"10.1177","volume":"23","author":[{"given":"Yi","family":"Wang","sequence":"first","affiliation":[{"name":"Cervicodynia\/Omalgia\/Lumbago\/Sciatica Department 2, Sichuan Provincial Orthopedic Hospital, Chengdu, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guogang","family":"Dai","sequence":"additional","affiliation":[{"name":"Cervicodynia\/Omalgia\/Lumbago\/Sciatica Department 2, Sichuan Provincial Orthopedic Hospital, Chengdu, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Postgraduate School, Chengdu Sport Institute, Chengdu, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangru","family":"Shang","sequence":"additional","affiliation":[{"name":"Postgraduate School, Chengdu Sport Institute, Chengdu, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Jiang","sequence":"additional","affiliation":[{"name":"College Hospital, Sichuan Agricultural University, Chengdu Campus, Chengdu, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengwu","family":"Li","sequence":"additional","affiliation":[{"name":"Cervicodynia\/Omalgia\/Lumbago\/Sciatica Department 2, Sichuan Provincial Orthopedic Hospital, Chengdu, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Huang","sequence":"additional","affiliation":[{"name":"Cervicodynia\/Omalgia\/Lumbago\/Sciatica Department 2, Sichuan Provincial Orthopedic Hospital, Chengdu, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiao","family":"Xia","sequence":"additional","affiliation":[{"name":"Cervicodynia\/Omalgia\/Lumbago\/Sciatica Department 2, Sichuan Provincial Orthopedic Hospital, Chengdu, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wei","sequence":"additional","affiliation":[{"name":"Cervicodynia\/Omalgia\/Lumbago\/Sciatica Department 2, Sichuan Provincial Orthopedic Hospital, Chengdu, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,9,12]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"crossref","unstructured":"Adams MA Roughley PJ (2006) What is intervertebral disc degeneration and what causes it? 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