{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T18:33:22Z","timestamp":1777660402500,"version":"3.51.4"},"reference-count":30,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T00:00:00Z","timestamp":1604188800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Genetic Testing and Molecular Biomarkers"],"published-print":{"date-parts":[[2020,11]]},"abstract":"\n Objective:<\/jats:title>\n \n Although genetic variants of key enzymes in the folic acid-methionine metabolic circulation, including methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) were thought to be related to the risk of recurrent pregnancy loss (RPL), the results of recent studies have been inconsistent. Therefore, the present retrospective case-control study was designed to explore whether the variants c.66A>G in\n MTRR<\/jats:italic>\n and c.677C>T and c.1298A>C in\n MTHFR<\/jats:italic>\n are associated with the susceptibility of RPL in Southeast Chinese women.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n In total, samples from 237 RPL patients and 618 healthy controls were collected and genotyped by fluorescent quantitative polymerase chain reaction. The frequencies of the variants were calculated and compared between the two groups. The relative risk of the various genotypes was further determined by calculating the odds ratio (OR) at a 95% confidence interval (CI).<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n A significant positive correlation was observed between the variants\n MTHFR<\/jats:italic>\n c.677C>T,\n MTHFR<\/jats:italic>\n c.1298A>C,\n MTRR<\/jats:italic>\n c.66A>G, and RPL susceptibility (\n MTHFR<\/jats:italic>\n c.677C>T, OR\u2009=\u20090.74, 95% CI\u2009=\u20090.58-0.95,\n p<\/jats:italic>\n \u2009=\u20090.02;\n MTHFR<\/jats:italic>\n c.1298A>C, OR\u2009=\u20091.39, 95% CI\u2009=\u20091.09-1.77,\n p<\/jats:italic>\n \u2009=\u20090.008;\n MTRR<\/jats:italic>\n c.66A>G, OR\u2009=\u20091.38, 95% CI\u2009=\u20091.10-1.73,\n p<\/jats:italic>\n \u2009=\u20090.006). Further analysis of the genotypic distributions of the three variants between the two groups showed that the\n MTHFR<\/jats:italic>\n c.677C>T heterozygote was associated with lower RPL risk, while the\n MTHFR<\/jats:italic>\n c.1298A>C variant and MTRR c.66A>G heterozygote were correlated with higher RPL risk (dominant model,\n MTHFR<\/jats:italic>\n c.677C>T, OR\u2009=\u20090.70, 95% CI\u2009=\u20090.52-0.95,\n p<\/jats:italic>\n \u2009=\u20090.02;\n MTHFR<\/jats:italic>\n c.1298A>C, OR\u2009=\u20091.39, 95% CI\u2009=\u20091.03-1.88,\n p<\/jats:italic>\n \u2009=\u20090.032;\n MTRR<\/jats:italic>\n c.66A>G, OR\u2009=\u20091.62, 95% CI\u2009=\u20091.20-2.19,\n p<\/jats:italic>\n \u2009=\u20090.002).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n MTHFR<\/jats:italic>\n c.677C>T and c.1298A>C and\n MTRR<\/jats:italic>\n c.66A>G were associated with RPL in Southeast Chinese women.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1089\/gtmb.2020.0106","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T23:34:11Z","timestamp":1604014451000},"page":"717-722","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Variants c.677 C>T, c.1298\u2009A>C in\n MTHFR<\/i>\n , and c.66\u2009A>G in\n MTRR<\/i>\n Affect the Occurrence of Recurrent Pregnancy Loss in Chinese Women"],"prefix":"10.1177","volume":"24","author":[{"given":"Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"Medical Genetics Center, Guangdong Women and Children Hospital, Guangzhou, P.R. China."},{"name":"Maternal and Children Metabolic-Genetic Disease Key Laboratory of Guangdong Women and Children Hospital, Guangzhou, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenli","family":"Zhan","sequence":"additional","affiliation":[{"name":"Medical Genetics Center, Guangdong Women and Children Hospital, Guangzhou, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianyi","family":"Du","sequence":"additional","affiliation":[{"name":"Medical Genetics Center, Guangdong Women and Children Hospital, Guangzhou, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wu","sequence":"additional","affiliation":[{"name":"Health Care Institute, Guangdong Women and Children Hospital, Guangzhou, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongke","family":"Ding","sequence":"additional","affiliation":[{"name":"Medical Genetics Center, Guangdong Women and Children Hospital, Guangzhou, P.R. China."},{"name":"Maternal and Children Metabolic-Genetic Disease Key Laboratory of Guangdong Women and Children Hospital, Guangzhou, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fenghua","family":"Liu","sequence":"additional","affiliation":[{"name":"Reproductive Center, Guangdong Women and Children Hospital, Guangzhou, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aihua","family":"Yin","sequence":"additional","affiliation":[{"name":"Medical Genetics Center, Guangdong Women and Children Hospital, Guangzhou, P.R. China."},{"name":"Maternal and Children Metabolic-Genetic Disease Key Laboratory of Guangdong Women and Children Hospital, Guangzhou, P.R. 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(2013) Definitions of infertility and recurrent pregnancy loss: a committee opinion. Fertil Steril, 1:63.","journal-title":"Fertil Steril"},{"key":"e_1_3_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ogc.2013.10.008"},{"key":"e_1_3_5_28_1","first-page":"933","article-title":"Folic acid antagonists during pregnancy and risk of birth defects","volume":"12","author":"Sharfstein JM","year":"2001","unstructured":"Sharfstein JM (2001) Folic acid antagonists during pregnancy and risk of birth defects. N Engl J Med, 12:933.","journal-title":"N Engl J Med"},{"key":"e_1_3_5_29_1","first-page":"e16763","article-title":"Methylenetetrahydrofolate reductase C677T and A1298C polymorphism in Iranian women with idiopathic recurrent pregnancy losses","volume":"7","author":"Yousefian E","year":"2014","unstructured":"Yousefian E, , Kardi MT, , Allahveisi A (2014) Methylenetetrahydrofolate reductase C677T and A1298C polymorphism in Iranian women with idiopathic recurrent pregnancy losses. 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