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This study evaluated the seroprevalence of antibodies against SARS-CoV-2 and its association with the degree of exposure and use of personal protective equipment by healthcare professionals dedicated to the treatment of patients with flu-like illnesses in the emergency room. The research team included an analysis of healthcare professionals who underwent enzyme-linked immunosorbent assay serological testing for SARS-CoV-2 between May 28 and June 26, 2020, in the emergency room of S\u00edrio-Liban\u00eas Hospital in S\u00e3o Paulo, Brazil. Participants answered individual questionnaires on occupational information, medical health history, and factors associated with exposure to the novel coronavirus. The questionnaire variables were compared based on the serological results. Of the 164 study participants, 96 (58.54%) reported at least 1 flu-like symptom and 42 (25.61%) presented serology results that were compatible with SARS-CoV-2 infection. The asymptomatic declared group accounted for 62 participants; of these, 8 (12.90%) had positive serology results (neutralizing antibody and IgG) for SARS-CoV-2. Data analysis showed a positive correlation with duration of work, safety in wearing and reusing personal protective equipment, and presence of anosmia, and showed a negative relationship with duration of mask use. Our findings suggest that the perception of symptoms by healthcare professionals is not a good screening parameter for the diagnosis of an infectious disease with respiratory symptoms, such as COVID-19. The main influencing factor for the control of infection is the elaboration of workflows and safety protocols based on simple and clear rules as well as investments in team training.<\/jats:p>","DOI":"10.1089\/hs.2022.0045","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T10:59:48Z","timestamp":1660301988000},"page":"359-367","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Seroprevalence of SARS-CoV-2 in Emergency Department Healthcare Workers at S\u00edrio-Liban\u00eas Hospital, Brazil"],"prefix":"10.1177","volume":"20","author":[{"given":"Ricardo","family":"Madureira","sequence":"first","affiliation":[{"name":"Ricardo Madureira, MD, is a Physician, Emergency Department, Hospital S\u00edrio-Liban\u00eas, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Aparecida","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Silvia Aparecida Ferreira, BSN, is a Nurse Care Coordinator, Emergency Department, Hospital S\u00edrio-Liban\u00eas, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magali Aldrin Lopes","family":"Marion","sequence":"additional","affiliation":[{"name":"Magali Aldrin Lopes Marion, BSN, is Nurse Manager, Emergency Department, Hospital S\u00edrio-Liban\u00eas, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiana","family":"Bettoni","sequence":"additional","affiliation":[{"name":"Fabiana Bettoni, PhD, is a Researcher, Molecular Oncology Center, Hospital S\u00edrio-Liban\u00eas, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Ganem","sequence":"additional","affiliation":[{"name":"Fernando Ganem, MD, PhD, is Hospital Director, Hospital S\u00edrio-Liban\u00eas, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anamaria Aranha","family":"Camargo","sequence":"additional","affiliation":[{"name":"Anamaria Aranha Camargo, PhD, is Research Manager, Molecular Oncology Center, Hospital S\u00edrio-Liban\u00eas, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian Valle","family":"Morinaga","sequence":"additional","affiliation":[{"name":"Christian Valle Morinaga, MD, PhD, is Physician Practice Manager, Emergency Department, Hospital S\u00edrio-Liban\u00eas, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2022,10,17]]},"reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1057\/s42214-020-00062-w"},{"key":"e_1_3_1_3_2","unstructured":"World Health Organization (WHO). 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