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For most HLIUs, maintaining readiness during times of inactivity is a challenge. In this case study, we describe a checklist approach to assess HLIU readiness to rapidly operate upon activation. This checklist includes readiness criteria in several domains, such as infrastructure, human resources, and material supplies, that are required to safely activate the unit at any time. The checklist audit tool was derived from a novel activation readiness checklist published by the biocontainment unit at The Johns Hopkins Hospital in Baltimore, Maryland. It was then adapted for the Irish healthcare setting and implemented at the Mater Misericordiae University Hospital, Ireland\u2019s current isolation facility. Results from the audit were also used to inform recommendations for the construction of a new HLIU to open in 2025. The audit tool is user friendly, practical, and focuses on the essential elements of readiness to ensure a successful rapid operation.<\/jats:p>","DOI":"10.1089\/hs.2023.0148","type":"journal-article","created":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T12:26:26Z","timestamp":1724415986000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Implementation of a High-Level Isolation Unit Readiness Checklist in the Irish Setting"],"prefix":"10.1177","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8062-9762","authenticated-orcid":false,"given":"Samata Salim","family":"Al Dowaiki","sequence":"first","affiliation":[{"name":"Samata Salim Al Dowaiki, MD, MRCP (UK), is an Internist and Infectious Diseases Specialist, Royal College of Physicians of Ireland, Dublin, Ireland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deirdre","family":"Morley","sequence":"additional","affiliation":[{"name":"Deirdre Morley, MBBCh, JFICMI, EDIC, is a Clinical Lead, National Isolation Unit, and a Consultant in Infectious Disease and Intensive Care Medicine;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris","family":"Agreiter","sequence":"additional","affiliation":[{"name":"Iris Agreiter, MSN, RN, is a Clinical Nurse Specialist, National Isolation Unit;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jocelyn J.","family":"Herstein","sequence":"additional","affiliation":[{"name":"Honey Vincent, RN, RNP, is a Staff Development Facilitator, National Isolation Unit; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honey","family":"Vincent","sequence":"additional","affiliation":[{"name":"James Woo, MBBCh, MRCPI, is Deputy Clinical Lead, National Isolation Unit, and a Consultant in Infectious Diseases; all at Mater Misericordiae University Hospital, Dublin, Ireland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Woo","sequence":"additional","affiliation":[{"name":"Jocelyn J. Herstein, PhD, MPH, is an Assistant Professor, Department of Environmental, Agricultural and Occupational Health, College of Public Health, and Director, National Emerging Special Pathogens Training and Education Center International Partnerships and Programs; both at University of Nebraska Medical Center, Omaha, NE."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,9,11]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0264644"},{"key":"e_1_3_2_3_2","unstructured":"UK Health Security Agency. High consequence infectious diseases (HCID). Published October 22 2018. Updated January 25 2023. Accessed May 6 2023. https:\/\/www.gov.uk\/guidance\/high-consequence-infectious-diseases-hcid"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2017.07.001"},{"key":"e_1_3_2_5_2","unstructured":"UK Health Security Agency. 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