{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T07:39:37Z","timestamp":1783150777597,"version":"3.54.6"},"reference-count":0,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p>\n                    Before conducting a phase 1\/2 clinical trial of a serotype 5 adenovirus encoding human aquaporin-1(AdhAQP1) for the treatment of radiation-damaged salivary glands, we have conducted a detailed toxicity and biodistribution study in adult rats. AdhAQP1 (2 \u00d7 10\n                    <jats:sup>8<\/jats:sup>\n                    \u20132 \u00d7 10\n                    <jats:sup>11<\/jats:sup>\n                    particles) was delivered to a single submandibular gland by retroductal cannulation. Administration of this vector resulted in no animal mortality or morbidities, and no adverse signs of clinical toxicity. In addition, over the 92-day time course of the study, with both male and female rats, there were no consistent treatment-related changes in serum indicators of hepatic, renal, and cardiac functions. Importantly, we also observed no vector-associated effects on either water consumption by, or hematocrit levels in, study animals. However, three suggestive mild gender-related response differences were seen. Female, but not male, rats exhibited small reductions in food consumption(\u223c10\u201315%) and body weight gain (5\u201310%), and evidence of persistent inflammation, after vector treatment. These were vector, but not dose, related. Three days after delivery of 2 \u00d7 10\n                    <jats:sup>11<\/jats:sup>\n                    particles of AdhAQP1, vector was detected primarily in the targeted gland; 9 of 10 samples from the targeted gland were positive, whereas only 5 of 90 nonoral samples were positive. There was no evidence of the generation of replication-competent adenovirus in saliva or blood samples. In aggregate, these findings show that localized delivery of AdhAQP1 to salivary glands appears to occur without significant toxicity.\n                  <\/jats:p>","DOI":"10.1089\/hum.2006.17.1122","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T08:57:36Z","timestamp":1163667456000},"page":"1122-1133","source":"Crossref","is-referenced-by-count":35,"title":["Toxicity and Biodistribution of a First-Generation Recombinant Adenoviral Vector, Encoding Aquaporin-1, After Retroductal Delivery to a Single Rat Submandibular Gland"],"prefix":"10.1177","volume":"17","author":[{"given":"Changyu","family":"Zheng","sequence":"first","affiliation":[{"name":"Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne M.","family":"Goldsmith","sequence":"additional","affiliation":[{"name":"Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumi","family":"Mineshiba","sequence":"additional","affiliation":[{"name":"Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Chiorini","sequence":"additional","affiliation":[{"name":"Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Kerr","sequence":"additional","affiliation":[{"name":"Toxicology Division, BioReliance Invitrogen Bioservices, Rockville, MD 20850."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin L.","family":"Wenk","sequence":"additional","affiliation":[{"name":"Toxicology Division, BioReliance Invitrogen Bioservices, Rockville, MD 20850."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly","family":"Vallant","sequence":"additional","affiliation":[{"name":"National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D.","family":"Irwin","sequence":"additional","affiliation":[{"name":"National Toxicology Program, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce J.","family":"Baum","sequence":"additional","affiliation":[{"name":"Gene Therapy and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2006,11,1]]},"container-title":["Human Gene Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/hum.2006.17.1122","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/hum.2006.17.1122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:15:25Z","timestamp":1779102925000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/hum.2006.17.1122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1089\/hum.2006.17.1122"],"URL":"https:\/\/doi.org\/10.1089\/hum.2006.17.1122","relation":{},"ISSN":["1043-0342","1557-7422"],"issn-type":[{"value":"1043-0342","type":"print"},{"value":"1557-7422","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11]]}}}