{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T08:36:23Z","timestamp":1782290183470,"version":"3.54.5"},"reference-count":0,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2006,11]]},"abstract":"\n Immunotherapeutic approaches involving genetic modification of T cells show promise in generating highly specific tumor-reactive effector cells for cancer treatment. Given the high affinity of Fc\n \n \u03f5<\/jats:italic>\n <\/jats:sub>\n RI (the subtype I Fc receptor for IgE) for IgE monoclonal antibody (mAb), modification of T cells with chimeric Fc\n \n \u03f5<\/jats:italic>\n <\/jats:sub>\n RI in combination with tumor-specific IgE mAbs is potentially a powerful and effective strategy to specifically target T cells to tumor cells. In this study, we retrovirally transduce human primary T cells with a cDNA encoding the extracellular domain of Fc\n \n \u03f5<\/jats:italic>\n <\/jats:sub>\n RI linked to the hinge and transmembrane domains of Fc\n \n \u03b3<\/jats:italic>\n <\/jats:sub>\n RI and the cytoplasmic domains of CD28 and T cell receptor \u03b6 chain (Fc\n \n \u03f5<\/jats:italic>\n <\/jats:sub>\n RI-CD28-\u03b6). We demonstrate that human T cells expressing Fc\n \n \u03f5<\/jats:italic>\n <\/jats:sub>\n RI-CD28-\u03b6, in the presence of tumor-specific IgE mAb recognizing mouse CD8 antigen (Ly- 2.1\n +<\/jats:sup>\n ), can specifically secrete cytokine, proliferate, and mediate cytotoxic function after antigen ligation. Furthermore, adoptive transfer of Fc\n \n \u03f5<\/jats:italic>\n <\/jats:sub>\n RI-CD28-\u03b6 cells incubated with anti-Ly-2.1 IgE mAb significantly enhances the survival of irradiated nonobese diabetic-severe combined immunodeficiency mice bearing Ly-2.1\n +<\/jats:sup>\n tumor compared with control mice. Thus, this set of experiments demonstrates that Fc\n \n \u03f5<\/jats:italic>\n <\/jats:sub>\n gene-engineered human T cells mediate effector function\n in vitro<\/jats:italic>\n and\n in vivo<\/jats:italic>\n in an IgE-dependent manner and thus a novel and valid approach for cancer therapy can now be further developed.\n <\/jats:p>","DOI":"10.1089\/hum.2006.17.1134","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T08:57:36Z","timestamp":1163667456000},"page":"1134-1143","source":"Crossref","is-referenced-by-count":21,"title":["Adoptive Transfer of Chimeric Fc\n \n \u03f5<\/i>\n <\/sub>\n RI Receptor Gene-Modified Human T Cells for Cancer Immunotherapy"],"prefix":"10.1177","volume":"17","author":[{"given":"Michele W.L.","family":"Teng","sequence":"first","affiliation":[{"name":"Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria 8006, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael H.","family":"Kershaw","sequence":"additional","affiliation":[{"name":"Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria 8006, Australia."},{"name":"Department of Pathology, University of Melbourne, Parkville, Victoria 3010, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob T.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria 8006, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Smyth","sequence":"additional","affiliation":[{"name":"Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria 8006, Australia."},{"name":"Department of Pathology, University of Melbourne, Parkville, Victoria 3010, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip K.","family":"Darcy","sequence":"additional","affiliation":[{"name":"Cancer Immunology Program, Peter MacCallum Cancer Centre, East Melbourne, Victoria 8006, Australia."},{"name":"Department of Pathology, University of Melbourne, Parkville, Victoria 3010, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2006,11,1]]},"container-title":["Human Gene Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/hum.2006.17.1134","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/hum.2006.17.1134","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:15:23Z","timestamp":1779102923000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/hum.2006.17.1134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1089\/hum.2006.17.1134"],"URL":"https:\/\/doi.org\/10.1089\/hum.2006.17.1134","relation":{},"ISSN":["1043-0342","1557-7422"],"issn-type":[{"value":"1043-0342","type":"print"},{"value":"1557-7422","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11]]}}}