{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:59:35Z","timestamp":1792706375364,"version":"4.5.0"},"reference-count":64,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2009,10,7]],"date-time":"2009-10-07T00:00:00Z","timestamp":1254873600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:p>\n                    Interest has increased in the use of exogenous stem cells to optimize lung repair and serve as carriers of a therapeutic gene for genetic airway diseases such as cystic fibrosis. We investigated the survival and engraftment of exogenous stem cells after intratracheal injection, in a murine model of acute epithelial airway injury already used in gene therapy experiments on cystic fibrosis. Embryonic stem cells and mesenchymal stem cells were intratracheally injected 24\u2009hr after 2% polidocanol administration, when epithelial airway injury was maximal. Stem cells were transfected with reporter genes immediately before administration. Reporter gene expression was analyzed in trachea\u2013lungs and bronchoalveolar lavage, using nonfluorescence, quantitative, and sensitive methods. Enzyme-linked immunosorbent assay quantitative results showed that 0.4 to 5.5% of stem cells survived in the injured airway. Importantly, no stem cells survived in healthy airway or in the epithelial lining fluid. Using 5-bromo-4-chloro-3-indolyl-\u03b2-d-galactopyranoside staining, transduced mesenchymal stem cells were detected in injured trachea and bronchi lumen. When the epithelium was spontaneously regenerated, the\n                    <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                    amount of engrafted mesenchymal stem cells from cell lines decreased dramatically. No stem cells from primary culture were located within the lungs at 7 days. This study demonstrated the feasibility of intratracheal cell delivery for airway diseases with acute epithelial injury.\n                  <\/jats:p>","DOI":"10.1089\/hum.2009.035","type":"journal-article","created":{"date-parts":[[2009,7,16]],"date-time":"2009-07-16T14:21:29Z","timestamp":1247754089000},"page":"1329-1343","source":"Crossref","is-referenced-by-count":60,"title":["Developing Cell Therapy Techniques for Respiratory Disease: Intratracheal Delivery of Genetically Engineered Stem Cells in a Murine Model of Airway Injury"],"prefix":"10.1177","volume":"20","author":[{"given":"Anne-Laure","family":"Leblond","sequence":"first","affiliation":[{"name":"Institut du Thorax","place":["France"]},{"name":"Universit\u00e9 de Nantes","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Naud","sequence":"additional","affiliation":[{"name":"Institut du Thorax","place":["France"]},{"name":"Universit\u00e9 de Nantes","place":["France"]},{"name":"CNRS","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Forest","sequence":"additional","affiliation":[{"name":"Institut du Thorax","place":["France"]},{"name":"Universit\u00e9 de Nantes","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clothilde","family":"Gourden","sequence":"additional","affiliation":[{"name":"In-Cell-Art","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Sagan","sequence":"additional","affiliation":[{"name":"Service d'Anatomo-Pathologie","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9n\u00e9dicte","family":"Romefort","sequence":"additional","affiliation":[{"name":"Institut du Thorax","place":["France"]},{"name":"Universit\u00e9 de Nantes","place":["France"]},{"name":"Institut du Thorax","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Mathieu","sequence":"additional","affiliation":[{"name":"Institut du Thorax","place":["France"]},{"name":"Universit\u00e9 de Nantes","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Delorme","sequence":"additional","affiliation":[{"name":"Microenvironnement de l'H\u00e9matopo\u00ef\u00e8se et Cellules Souches","place":["France"]},{"name":"Universit\u00e9 de Tours","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Collin","sequence":"additional","affiliation":[{"name":"CHRU de Tours Laboratoire de Biochimie et Biologie Mol\u00e9culaire","place":["France"]},{"name":"Universit\u00e9 Fran\u00e7ois Rabelais de Tours","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Christophe","family":"Pag\u00e8s","sequence":"additional","affiliation":[{"name":"CHRU de Tours Laboratoire de Biochimie et Biologie Mol\u00e9culaire","place":["France"]},{"name":"Universit\u00e9 Fran\u00e7ois Rabelais de Tours","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Senseb\u00e9","sequence":"additional","affiliation":[{"name":"Microenvironnement de l'H\u00e9matopo\u00ef\u00e8se et Cellules Souches","place":["France"]},{"name":"Universit\u00e9 de Tours","place":["France"]},{"name":"Etablissement Fran\u00e7ais du Sang Centre-Atlantique","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Pitard","sequence":"additional","affiliation":[{"name":"Institut du Thorax","place":["France"]},{"name":"Universit\u00e9 de Nantes","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Lemarchand","sequence":"additional","affiliation":[{"name":"Institut du Thorax","place":["France"]},{"name":"Universit\u00e9 de Nantes","place":["France"]},{"name":"Institut du Thorax","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2009,10,7]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)06532-5"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0198"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-006-0382-7"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2002.0561"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2005.05.007"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.24.6.4217"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302015"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0166"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.297.5585.1299"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0138"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1996.7.15-1837"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00197.2006"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07041"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652307781369119"},{"key":"e_1_3_3_16_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2004-0079RC"},{"key":"e_1_3_3_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.4.645"},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199606)167:3<523::AID-JCP16>3.0.CO;2-4"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240600855905"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/55.1.24"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116155"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.3.1855"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0297-216"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.04.007"},{"key":"e_1_3_3_25_1","unstructured":"Institute of Laboratory Animal Research Commission on Life Sciences National Research Council. 1996. Guide for the Care and Use of Laboratory Animals. National Academies Press: Washington D.C.NIH Publication No. 85-23."},{"key":"e_1_3_3_26_1","first-page":"223","article-title":"Establishment and characterization of a new osteogenic cell line (MOS-J) from a spontaneous C57BL\/6J mouse osteosarcoma","volume":"16","author":"Joliat M.J.","year":"2002","unstructured":"Joliat M.J., , Umeda S., , Lyons B.L., , Lynes M.A., , Shultz L.D. 2002. Establishment and characterization of a new osteogenic cell line (MOS-J) from a spontaneous C57BL\/6J mouse osteosarcoma. In Vivo, 16:223\u2013228.","journal-title":"In Vivo"},{"key":"e_1_3_3_27_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1349.015"},{"key":"e_1_3_3_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00328-2"},{"key":"e_1_3_3_29_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2004-0319OC"},{"key":"e_1_3_3_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1994.76.6.2840"},{"key":"e_1_3_3_31_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20070073"},{"key":"e_1_3_3_32_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200801-009AW"},{"key":"e_1_3_3_33_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200502-309OC"},{"key":"e_1_3_3_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02375"},{"key":"e_1_3_3_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-7-145"},{"key":"e_1_3_3_36_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200708-1233OC"},{"key":"e_1_3_3_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116175"},{"key":"e_1_3_3_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040269"},{"key":"e_1_3_3_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02446"},{"key":"e_1_3_3_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.18.8424"},{"key":"e_1_3_3_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.01.015"},{"key":"e_1_3_3_42_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1998.9.18-2661"},{"key":"e_1_3_3_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.11.4857"},{"key":"e_1_3_3_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.211"},{"key":"e_1_3_3_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302518"},{"key":"e_1_3_3_46_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2007.0194"},{"key":"e_1_3_3_47_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0465"},{"key":"e_1_3_3_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2008.03.009"},{"key":"e_1_3_3_49_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0128"},{"key":"e_1_3_3_50_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0705"},{"key":"e_1_3_3_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.1.592-600.1999"},{"key":"e_1_3_3_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.20574"},{"key":"e_1_3_3_53_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200610-1491OC"},{"key":"e_1_3_3_54_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00173.2001"},{"key":"e_1_3_3_55_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200706-859OC"},{"key":"e_1_3_3_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2395"},{"key":"e_1_3_3_57_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1074807"},{"key":"e_1_3_3_58_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406266102"},{"key":"e_1_3_3_59_1","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2002.0662"},{"key":"e_1_3_3_60_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.1997.8.13-1545"},{"key":"e_1_3_3_61_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00050.2007"},{"key":"e_1_3_3_62_1","first-page":"336","article-title":"Identification of a bone marrow-derived epithelial-like population capable of repopulating injured mouse airway epithelium","volume":"119","author":"Wong A.P.","year":"2009","unstructured":"Wong A.P., , Keating A., , Lu W.Y., , Duchesneau P., , Wang X., , Sacher A., , Hu J., , Waddell T.K. 2009. Identification of a bone marrow-derived epithelial-like population capable of repopulating injured mouse airway epithelium. J. Clin. Invest., 119:336\u2013348.","journal-title":"J. Clin. Invest."},{"key":"e_1_3_3_63_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00431.2006"},{"key":"e_1_3_3_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2302"},{"key":"e_1_3_3_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0997-871"}],"container-title":["Human Gene Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/hum.2009.035","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/hum.2009.035","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/hum.2009.035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:16:46Z","timestamp":1779103006000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/hum.2009.035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10,7]]},"references-count":64,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2009,11]]}},"alternative-id":["10.1089\/hum.2009.035"],"URL":"https:\/\/doi.org\/10.1089\/hum.2009.035","relation":{},"ISSN":["1043-0342","1557-7422"],"issn-type":[{"value":"1043-0342","type":"print"},{"value":"1557-7422","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,10,7]]}}}