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We have previously shown that a 500-bp fragment from the proximal\n                    <jats:italic toggle=\"yes\">Was<\/jats:italic>\n                    gene promoter in a lentiviral vector (LV) was sufficient to achieve more than 100-fold higher levels of Wiskott-Aldrich syndrome protein in HCs than in nonhematopoietic cells (non-HCs). We show now that this differential was reduced up to 10 times when the enhanced green fluorescent protein gene (\n                    <jats:italic toggle=\"yes\">eGFP<\/jats:italic>\n                    ) was expressed instead of\n                    <jats:italic toggle=\"yes\">Was<\/jats:italic>\n                    in the same LV backbone. Insertion of\n                    <jats:italic toggle=\"yes\">Was<\/jats:italic>\n                    cDNA sequences downstream of\n                    <jats:italic toggle=\"yes\">eGFP<\/jats:italic>\n                    in these LVs had a negative effect on transgene expression. This effect varied in different cell types but, overall,\n                    <jats:italic toggle=\"yes\">Was<\/jats:italic>\n                    cDNA sequences increased the hematopoietic specificity of\n                    <jats:italic toggle=\"yes\">Was<\/jats:italic>\n                    promoter-driven LV. We have characterized the minimal fragment required to increase hematopoietic specificity and have demonstrated that the mechanism involves\n                    <jats:italic toggle=\"yes\">Was<\/jats:italic>\n                    promoter regulation and RNA processing. In addition, we have shown that\n                    <jats:italic toggle=\"yes\">Was<\/jats:italic>\n                    cDNA sequences interfere with the enhancer activity of the woodchuck posttranscriptional regulatory element. These results represent the first data showing the role of\n                    <jats:italic toggle=\"yes\">Was<\/jats:italic>\n                    intragenic sequences in gene regulation.\n                  <\/jats:p>","DOI":"10.1089\/hum.2009.118","type":"journal-article","created":{"date-parts":[[2009,7,24]],"date-time":"2009-07-24T14:02:05Z","timestamp":1248444125000},"page":"1279-1290","source":"Crossref","is-referenced-by-count":5,"title":["<i>Was<\/i>\n                    cDNA Sequences Modulate Transgene Expression of\n                    <i>Was<\/i>\n                    Promoter-Driven Lentiviral Vectors"],"prefix":"10.1177","volume":"20","author":[{"given":"Miguel G.","family":"Toscano","sequence":"first","affiliation":[{"name":"Institute of Parasitology and Biomedicine L\u00f3pez Neyra-CSIC","place":["Spain"]},{"name":"Temple University School of 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