{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T12:10:05Z","timestamp":1779106205405,"version":"3.51.4"},"reference-count":65,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2010,9,22]],"date-time":"2010-09-22T00:00:00Z","timestamp":1285113600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:p>\n                    It has been demonstrated that an active vaccination strategy with protein- or DNA-based epitope vaccines composed of the immunodominant self B cell epitope of amyloid-\u03b2\n                    <jats:sub>42<\/jats:sub>\n                    (A\u03b2\n                    <jats:sub>42<\/jats:sub>\n                    ) and a non-self T helper (Th) cell epitope is an immunotherapeutic approach to preventing or treating Alzheimer's disease (AD). As a DNA-based epitope vaccine, we used a plasmid encoding three copies of A\u03b2\n                    <jats:sub>1\u201311<\/jats:sub>\n                    and Th cell epitope, PADRE (p3A\u03b2\n                    <jats:sub>1\u201311<\/jats:sub>\n                    -PADRE). We have previously reported that three copies of component of complement C3d (3C3d) acts as a molecular adjuvant significantly enhancing immune responses in wild-type mice of the H2\n                    <jats:sup>b<\/jats:sup>\n                    haplotype immunized with p3A\u03b2\n                    <jats:sub>1\u201311<\/jats:sub>\n                    -PADRE. Here, we tested the efficacy of p3A\u03b2\n                    <jats:sub>1\u201311<\/jats:sub>\n                    -PADRE and the same vaccine fused with 3C3d (p3A\u03b2\n                    <jats:sub>1\u201311<\/jats:sub>\n                    -PADRE-3C3d) in a transgenic (Tg) mouse model of AD (Tg2576) of the H2\n                    <jats:sup>bxs<\/jats:sup>\n                    immune haplotype. The overall responses to both vaccines were very weak in Tg2576 mice despite the fact that the 3C3d molecular adjuvant significantly enhanced the anti-A\u03b2 response to 3A\u03b2\n                    <jats:sub>1\u201311<\/jats:sub>\n                    -PADRE. Importantly, generation of low antibody responses was associated with the strain of amyloid precursor protein Tg mice rather than with a molecular adjuvant, as a p3A\u03b2\n                    <jats:sub>1\u201311<\/jats:sub>\n                    -PADRE-3C3d vaccine induced significantly higher antibody production in another AD mouse model, 3xTg-AD of the H2\n                    <jats:sup>b<\/jats:sup>\n                    haplotype. Finally, this study demonstrated that low concentrations of antibodies generated by both DNA vaccines were not sufficient for the reduction of A\u03b2 pathology in the brains of vaccinated Tg2576 animals, confirming previous reports from preclinical studies and the AN-1792 clinical trials, which concluded that the concentration of anti-A\u03b2 antibodies may be essential for the reduction of AD pathology.\n                  <\/jats:p>","DOI":"10.1089\/hum.2009.219","type":"journal-article","created":{"date-parts":[[2010,6,8]],"date-time":"2010-06-08T14:58:32Z","timestamp":1276009112000},"page":"1569-1576","source":"Crossref","is-referenced-by-count":8,"title":["Low Concentrations of Anti-A\u03b2 Antibodies Generated in Tg2576 Mice by DNA Epitope Vaccine Fused with 3C3d Molecular Adjuvant Do Not Affect AD Pathology"],"prefix":"10.1177","volume":"21","author":[{"given":"Nina","family":"Movsesyan","sequence":"first","affiliation":[{"name":"Institute for Molecular Medicine"},{"name":"University of California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayk","family":"Davtyan","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikayel","family":"Mkrtichyan","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Petrushina","sequence":"additional","affiliation":[{"name":"University of California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tigran","family":"Tiraturyan","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ted","family":"Ross","sequence":"additional","affiliation":[{"name":"University of Pittsburgh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael G.","family":"Agadjanyan","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine"},{"name":"University of California"},{"name":"Russian Academy of Medical Sciences","place":["Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anahit","family":"Ghochikyan","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"Cribbs","sequence":"additional","affiliation":[{"name":"University of California"},{"name":"University of California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2010,9,22]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.3.1580"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80433-1"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.3.1625"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00269-4"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/78682"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000148604.77591.67"},{"key":"e_1_3_3_8_1","volume-title":"8th International Conference \u201cAlzheimer's and Parkinson's Disease: Progress and New Perspectives\u201d","author":"Boche D.","year":"2007","unstructured":"Boche D., , DeBeer S., , Cox A., , Wilkinson D., , Holmes C., , Neal J., , Love S., , Esiri M., , Bridges L., , Weller R., , Nicoll J.A. 2007. 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