{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:44:03Z","timestamp":1790977443147,"version":"4.1.0"},"reference-count":24,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2010,9,9]],"date-time":"2010-09-09T00:00:00Z","timestamp":1283990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Human Gene Therapy"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:p>\n                    We have investigated whether systemic delivery of an oncolytic adenovirus, Ad.sT\u03b2RFc, expressing the soluble form of transforming growth factor-\u03b2 receptor II fused with human immunoglobulin Fc fragment (sTGF\u03b2RIIFc), could inhibit breast cancer bone metastasis in a mouse model. MDA-MB-231 (human breast cancer) cells were inoculated into the left heart ventricles of nude mice. Once the skeletal tumors were visible by X-rays, mice were intravenously injected with either buffer, Ad.sT\u03b2RFc, Ad(E1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ).sT\u03b2RFc (a replication-deficient adenovirus expressing sTGF\u03b2RIIFc), or Ad.luc2 (a replicating adenovirus expressing firefly luciferase gene). On days 2 and 7 after viral injections, viral replication and sTGF\u03b2RIIFc expression were detected in the skeletal tumors in Ad.sT\u03b2RFc-treated group; only viral replication in Ad.luc2 group, and sTGF\u03b2RIIFc expression in the Ad(E1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ).sT\u03b2RFc group, were detected. To examine the therapeutic effects, buffer or various viral vectors were administered on days 4 and 7 after intracardiac injection of MDA-MB-231 cells. On day 28, X-ray radiography showed a highly significant reduction in lesion size by Ad.sT\u03b2RFc, a significant reduction by Ad.luc2, and some reduction by Ad(E1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ).sT\u03b2RFc. Goldner's trichrome and hematoxylin\u2013eosin staining of the bone sections revealed a significant reduction of tumor burden in the Ad.sT\u03b2RFc group, but not in the Ad(E1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ).sT\u03b2RFc or Ad.luc2 group. There were significant reductions in free calcium levels by Ad.sT\u03b2RFc, Ad(E1\n                    <jats:sup>\u2212<\/jats:sup>\n                    ).sT\u03b2RFc, and Ad.luc2; however, only in the Ad.sT\u03b2RFc group were calcium levels reduced to the normal values. These results suggest that concomitant viral replication and sTGF\u03b2RIIFc production are important to inhibit bone metastasis and osteolysis, and that Ad.sT\u03b2RFc could be developed for targeting breast cancer bone metastases.\n                  <\/jats:p>","DOI":"10.1089\/hum.2010.018","type":"journal-article","created":{"date-parts":[[2010,8,16]],"date-time":"2010-08-16T10:58:06Z","timestamp":1281956286000},"page":"1623-1629","source":"Crossref","is-referenced-by-count":44,"title":["Systemic Delivery of an Oncolytic Adenovirus Expressing Soluble Transforming Growth Factor-\n                    <i>\u03b2<\/i>\n                    Receptor II\u2013Fc Fusion Protein Can Inhibit Breast Cancer Bone Metastasis in a Mouse Model"],"prefix":"10.1177","volume":"21","author":[{"given":"Zebin","family":"Hu","sequence":"first","affiliation":[{"name":"University of Chicago"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Chicago"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Guise","sequence":"additional","affiliation":[{"name":"Indiana University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prem","family":"Seth","sequence":"additional","affiliation":[{"name":"University of Chicago"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2010,9,9]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.7.1.5163"},{"key":"e_1_3_3_3_1","unstructured":"American Cancer Society. 2010. Cancer Facts & Figures. http:\/\/www.cancer.org\/docroot\/STT\/stt_0.asp. 2010August."},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/156800907780058862"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/mthe.2000.0206"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2009.72"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.4161\/cbt.4.3.1566"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506517102"},{"key":"e_1_3_3_9_1","first-page":"889","article-title":"Cytotoxic effects of adenovirus-mediated wild-type p53 protein expression in normal and tumor mammary epithelial cells","volume":"1","author":"Katayose D.","year":"1995","unstructured":"Katayose D., , Gudas J., , Nguyen H., , Srivastava S., , Cowan K.H., , Seth P. 1995. Cytotoxic effects of adenovirus-mediated wild-type p53 protein expression in normal and tumor mammary epithelial cells. Clin. Cancer Res., 1:889\u2013897.","journal-title":"Clin. Cancer Res."},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-07-0234"},{"key":"e_1_3_3_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1943"},{"key":"e_1_3_3_12_1","doi-asserted-by":"publisher","DOI":"10.1677\/erc.1.01172"},{"key":"e_1_3_3_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209064"},{"key":"e_1_3_3_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2008.316"},{"key":"e_1_3_3_15_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-126920"},{"key":"e_1_3_3_16_1","first-page":"1","volume-title":"Adenoviruses: Basic Biology to Gene Therapy","author":"Seth P.","year":"1999","unstructured":"Seth P. 1999. Adenoviruses: Basic Biology to Gene Therapy. R.G. Landes: Austin, TX, 1\u2013314."},{"key":"e_1_3_3_17_1","first-page":"1346","article-title":"Adenovirus-mediated gene transfer to human breast tumor cells: An approach for cancer gene therapy and bone marrow purging","volume":"56","author":"Seth P.","year":"1996","unstructured":"Seth P., , Brinkmann U., , Schwartz G.N., , Katayose D., , Gress R., , Pastan I., , Cowan K. 1996. Adenovirus-mediated gene transfer to human breast tumor cells: An approach for cancer gene therapy and bone marrow purging. Cancer Res., 56:1346\u20131351.","journal-title":"Cancer Res."},{"key":"e_1_3_3_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2006.17.1152"},{"key":"e_1_3_3_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2007.01.013"},{"key":"e_1_3_3_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1469"},{"key":"e_1_3_3_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.01.016"},{"key":"e_1_3_3_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-05-0125"},{"key":"e_1_3_3_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200215333"},{"key":"e_1_3_3_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3523"},{"key":"e_1_3_3_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1830978100"}],"container-title":["Human Gene Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/hum.2010.018","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/hum.2010.018","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/hum.2010.018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T11:15:52Z","timestamp":1779102952000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/hum.2010.018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,9]]},"references-count":24,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.1089\/hum.2010.018"],"URL":"https:\/\/doi.org\/10.1089\/hum.2010.018","relation":{},"ISSN":["1043-0342","1557-7422"],"issn-type":[{"value":"1043-0342","type":"print"},{"value":"1557-7422","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,9]]}}}