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The aims of this work are as follows: (a) to evaluate a mouse model of DES induced by clinically relevant doses of radiation, and (b) to evaluate the protective effect of erythropoietin (Epo) in preventing DES. C3H female mice were subjected to five sessions of RTx, with or without pre-RTx retroductal administration of the AdLTR2EF1a-hEPO (AdEpo) vector in the salivary glands (SG), and compared with na\u00efve controls at Day 10 (10d) (8\u2009Gy fractions) and 56 days (56d) (6\u2009Gy fractions) after RTx treatment. Mice were tested for changes in lacrimal glands (LG), tear secretion (phenol red thread), weight, hematocrit (Hct), and markers of inflammation, as well as microvessels and oxidative damage. Tear secretion was reduced in both RTx groups, compared to controls, by 10d. This was also seen at 56d in RTx but not AdEpo+RTx group. Hct was significantly higher in all AdEpo+RTx mice at 10d and 56d. Corneal epithelium was significantly thinner at 10d in the RTx group compared with AdEpo+RTx or the control mice. There was a significant reduction at 10d in vascular endothelial growth factor (VEGF)-R2 in LG in the RTx group that was prevented in the AdEpo+RTx group. In conclusion, RTx is able to induce DES in mice. AdEpo administration protected corneal epithelia and resulted in some recovery of LG function, supporting the value of further studies using gene therapy for extraglandular diseases.<\/jats:p>","DOI":"10.1089\/hum.2012.111","type":"journal-article","created":{"date-parts":[[2013,2,12]],"date-time":"2013-02-12T12:36:29Z","timestamp":1360672589000},"page":"417-423","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["Recovery of Radiation-Induced Dry Eye and Corneal Damage by Pretreatment with Adenoviral Vector-Mediated Transfer of Erythropoietin to the Salivary Glands in Mice"],"prefix":"10.1177","volume":"24","author":[{"given":"Eduardo M.","family":"Rocha","sequence":"first","affiliation":[{"name":"S\u00e3o Paulo University","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana P.","family":"Cotrim","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyu","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola Perez","family":"Riveros","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce J.","family":"Baum","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Chiorini","sequence":"additional","affiliation":[{"name":"National Institutes of Health"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,2,12]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.06209"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2008.07014.x"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2008.07014.x"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411990100030201"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1987.14"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejcn.1601186"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2007.10.028"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5001546"},{"key":"e_1_3_3_10_1","first-page":"1477","article-title":"Decreased expression of antioxidant enzymes in the conjunctival epithelium of dry eye (Sjogren's syndrome) and its possible contribution to the development of ocular surface oxidative injuries","volume":"23","author":"Cejkova J.","year":"2008","unstructured":"Cejkova J., , Ardan T., , Simonova Z. et al. 2008. 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